ID A0A0F4GWH2_9PEZI Unreviewed; 646 AA.
AC A0A0F4GWH2;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=General negative regulator of transcription subunit {ECO:0000256|PIRNR:PIRNR005290};
GN ORFNames=TI39_contig279g00034 {ECO:0000313|EMBL:KJY01757.1};
OS Zymoseptoria brevis.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Zymoseptoria.
OX NCBI_TaxID=1047168 {ECO:0000313|EMBL:KJY01757.1, ECO:0000313|Proteomes:UP000033647};
RN [1] {ECO:0000313|EMBL:KJY01757.1, ECO:0000313|Proteomes:UP000033647}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Zb18110 {ECO:0000313|EMBL:KJY01757.1,
RC ECO:0000313|Proteomes:UP000033647};
RA Grandaubert J., Bhattacharyya A., Stukenbrock E.H.;
RT "RNA-seq based gene annotation and comparative genomics of four
RT Zymoseptoria species reveal species-specific pathogenicity related genes
RT and transposable element activity.";
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as component of the CCR4-NOT core complex, which in the
CC nucleus seems to be a general transcription factor, and in the
CC cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT
CC protein subcomplex negatively regulates the basal and activated
CC transcription of many genes. Preferentially affects TC-type TATA
CC element-dependent transcription. Could directly or indirectly inhibit
CC component(s) of the general transcription machinery.
CC {ECO:0000256|PIRNR:PIRNR005290}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR005290}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123, ECO:0000256|PIRNR:PIRNR005290}.
CC -!- SIMILARITY: Belongs to the CNOT2/3/5 family.
CC {ECO:0000256|ARBA:ARBA00007682, ECO:0000256|PIRNR:PIRNR005290}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJY01757.1}.
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DR EMBL; LAFY01000271; KJY01757.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0F4GWH2; -.
DR STRING; 1047168.A0A0F4GWH2; -.
DR OrthoDB; 10289at2759; -.
DR Proteomes; UP000033647; Unassembled WGS sequence.
DR GO; GO:0030015; C:CCR4-NOT core complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; IEA:UniProtKB-UniRule.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR Gene3D; 2.30.30.1020; CCR4-NOT complex subunit 2/3/5, C-terminal domain; 1.
DR InterPro; IPR038635; CCR4-NOT_su2/3/5_C_sf.
DR InterPro; IPR012270; CCR4-NOT_su3/5.
DR InterPro; IPR040168; Not2/3/5.
DR InterPro; IPR007282; NOT2/3/5_C.
DR InterPro; IPR007207; Not_N.
DR PANTHER; PTHR23326; CCR4 NOT-RELATED; 1.
DR PANTHER; PTHR23326:SF1; CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 3; 1.
DR Pfam; PF04153; NOT2_3_5_C; 1.
DR Pfam; PF04065; Not3; 1.
DR PIRSF; PIRSF005290; NOT_su_3_5; 2.
PE 3: Inferred from homology;
KW Activator {ECO:0000256|PIRNR:PIRNR005290};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|PIRNR:PIRNR005290};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR005290};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000033647};
KW Repressor {ECO:0000256|ARBA:ARBA00022491, ECO:0000256|PIRNR:PIRNR005290};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|PIRNR:PIRNR005290};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|PIRNR:PIRNR005290}.
FT DOMAIN 3..231
FT /note="CCR4-Not complex component Not N-terminal"
FT /evidence="ECO:0000259|Pfam:PF04065"
FT DOMAIN 514..642
FT /note="NOT2/NOT3/NOT5 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04153"
FT REGION 233..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 350..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 40..96
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 124..158
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 264..296
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 412..432
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 440..454
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 646 AA; 72126 MW; AF038976BF267E3F CRC64;
MAARKLQQEI DKEFKRVAEG VALFEGIYEK LTLSTNPSQK EKLEDSLKKE IKKLQRSRDK
IKGWASQNDI KDKKPLQDQR KLIETQMEKF KAVEKEMKTK AFSKEGLNAA AKLDPKEKEK
MEVAQFLSDM VDELGRQVES QEAEVEAIQA TLKKGKKDSG KADRMAELER MVERHKWHTG
KLEILLRSLE NGSVEVEQVK EIDDGIKYYV EQNQEVDFME DDTIYDDLAL EEEEDQFGIA
GDNDKVSSQD NQSIQDEPPE HEPTRTTSAP SIKTKSTSIS ENTAGVRRPS VQMKSPLPAL
STLHASLPGN VNGKVAETMR PAPLPTIPTG QPLKYASAAA AAASSVGIAP LPPPPAAAKT
ASPAPAPAQP VAASPARPMA AQPAKAAEAL TKQPSPAPKE EVKVPAPKAP TPVEQVSSQA
SKVSSVKPSP QVARVQPQAA PPAESSSKAR QSPSPDSGIA GIALSHRDSA QDEEEEESIY
HLPSSLADLL GSFEETKQRA KSDQPFDLNM MNASRVTCPT PLDAEKPNHY KPQNPYAYTP
AHYPQEPLGI FDDPRLYSRI DTDSLFYAFY YRQGTYQQYL AAKALKSQSW RFHKQYQTWF
QRHEEPKNIT EEFEQGTYRF FDYESTWMNR RKADFKFAYK FLEDDL
//
