ID A0A0F4KR76_9LACO Unreviewed; 693 AA.
AC A0A0F4KR76;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00255};
GN Name=glyS {ECO:0000256|HAMAP-Rule:MF_00255,
GN ECO:0000313|EMBL:KJY48910.1};
GN ORFNames=JG29_07300 {ECO:0000313|EMBL:KJY48910.1};
OS Bombilactobacillus mellis.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Bombilactobacillus.
OX NCBI_TaxID=1218508 {ECO:0000313|EMBL:KJY48910.1, ECO:0000313|Proteomes:UP000033695};
RN [1] {ECO:0000313|EMBL:KJY48910.1, ECO:0000313|Proteomes:UP000033695}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hon2 {ECO:0000313|EMBL:KJY48910.1,
RC ECO:0000313|Proteomes:UP000033695};
RA Ellegaard K.M., Tamarit D., Javelind E., Olofsson T., Andersson S.G.,
RA Vasquez A.;
RT "Comparative genomics of the lactic acid bacteria isolated from the honey
RT bee gut.";
RL Submitted (DEC-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201,
CC ECO:0000256|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJY48910.1}.
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DR EMBL; JXBZ01000007; KJY48910.1; -; Genomic_DNA.
DR RefSeq; WP_045922579.1; NZ_KQ033877.1.
DR AlphaFoldDB; A0A0F4KR76; -.
DR STRING; 1218508.JG29_07300; -.
DR PATRIC; fig|1218508.4.peg.748; -.
DR HOGENOM; CLU_007220_2_2_9; -.
DR OrthoDB; 9775440at2; -.
DR Proteomes; UP000033695; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR NCBIfam; TIGR00211; glyS; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01045; TRNASYNTHGB.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00255};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00255};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00255};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Reference proteome {ECO:0000313|Proteomes:UP000033695}.
SQ SEQUENCE 693 AA; 78709 MW; 7A6960239E56BA43 CRC64;
MGKTFLLEIG LEEMPAHVVS SASLQLKQRV SDYLTAQKLE FQDIQAYSTP RRLAVLVHEL
ADQQPDENIA VKGPAKKIAL DKEGHWSKAA QGFVRGQKMT TDDIYFQDVK GTEYVYVQKH
IAGQSAVQVL QGLIEPIKAM TFPTRMRWNK FDFAYIRPIH WLVAMLDQQV IDLKLLNITA
NNQTRGHRFL GSTITLATAQ DYQKALADDY VIVDAQERKN LIRQQIAALA AENNWQVDVD
EDLLEEVNNL VEYPTAFAGK FSEKYLEMPE EVLITSMKDN QRYFYVRKKD GSLAPNFIAV
RNGNQEYLEN VIAGNEKVLV ARLDDAAFFF AEDQKHDLNY YVRQLQHVTF HDKIGSMSEK
MARTKVIADL LAQKLQLSSS DTAAVLRAAE IYKFDLVTDM VGEFAELQGV MGEKYALLMG
EKPAVAQAIR EHYLPNSAEG QLPQSLVGSV LAVADKLESI MSFFAVDLIP NGSNDPYGLR
RQALGIIRIL EQRNWQLNLH QLQNEIVAAY QKQFSTLKFD YFKHQAEVDE FLIDRIRQLF
SQDQVAHDLI DTVVALPSLD PTAMQQLVQV ISHHQTDPDF KGAIEALTRV LRITQKAPLT
DSTDLAVDPQ LFQTDSEKQL YQAVQACQAD FKDSDFEQKY QALAHLEPVI TDYFTVNMVM
DPNPEIRNNR LHQLQQLATL IKQLGDLNQL IVK
//
