ID A0A0F5VF45_9GAMM Unreviewed; 1151 AA.
AC A0A0F5VF45;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=KY46_06105 {ECO:0000313|EMBL:KKD00678.1};
OS Photobacterium halotolerans.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Photobacterium.
OX NCBI_TaxID=265726 {ECO:0000313|EMBL:KKD00678.1, ECO:0000313|Proteomes:UP000033633};
RN [1] {ECO:0000313|EMBL:KKD00678.1, ECO:0000313|Proteomes:UP000033633}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MELD1 {ECO:0000313|EMBL:KKD00678.1,
RC ECO:0000313|Proteomes:UP000033633};
RA Mathew D.C., Huang C.-C.;
RT "Mercury Reductase activity and rhizosphere competence traits in the genome
RT of root associated Photobacterium halotolerans MELD1.";
RL Submitted (DEC-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKD00678.1}.
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DR EMBL; JWYV01000003; KKD00678.1; -; Genomic_DNA.
DR RefSeq; WP_046219745.1; NZ_JWYV01000003.1.
DR AlphaFoldDB; A0A0F5VF45; -.
DR STRING; 265726.KY46_06105; -.
DR PATRIC; fig|265726.11.peg.3123; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000033633; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000033633}.
FT DOMAIN 619..780
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 802..955
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1151 AA; 129365 MW; 716B1FAB14B881C2 CRC64;
MTTHTLFSLP LPNKAGDKRF VGNVSGAALA VAIAGLAKNH AGTVLAVVPD TQTALRLQPE
ISQFSQAEVN IFPDWETLPY DSFSPHQDII SDRLARLYQL PTQQDGVLLV PVSTLLQRLT
PRSFIHQHAL LVSKGDRLSL EKLRLQLEAA GYRHVDQVME HGEYASRGSL LDLFPMGNNE
PFRIDFFDDE VDSIRQFDPE TQLSTGEIDN IHLLPAHEFP TDEVAVENFR MRWREQFEAR
REPESIYQQV SKRTWPAGIE YWQPLFFDHT DTLFDYLPDN TLLLTIGDLE SAADHFLHDA
EYRFQERRVD PLRPLLEPKA LWLTTAEMFS GFKTLPQVKL SKAPEEDKAG RFNPAISPVP
ALTINHQLKE PLSALRQFQE SFTGKIIFSV ASEGRREALL DLLARIKLRP MVCHSLEEAM
SAASQYTLVI GAAEQGFILD EPALAFICES DLLGERVIQR RRREDKRTVS SDTIIRNLAE
LKIGQPVVHL DHGIGRYQGL QTLEAGGITT EYVTLEYQGG AKLYVPVASL HLISRYSGGA
EEAAPIHKLG SESWQKARKK AAEKVRDVAA ELLDVYAKRE LKPGFKFKLD REAYADFCTG
FPFEETHDQA MAINAVLSDM CQAKAMDRLV CGDVGFGKTE VAMRAAFVSV DNNKQVTVLV
PTTLLAQQHF ENFRDRFANT PVRVEVLSRF KTAKEQKAIM QDVAEGKIDI LIGTHKLLNA
EVKYQDLGLL IVDEEHRFGV RQKEKLKSLR ADVDILTLTA TPIPRTLNMA MSGMRDLSII
ATPPARRLAI KTFVREREDL IIREAVLREI MRGGQVYFLH NDVESIEKTA QDLATLIPEA
RITTAHGQMR ERELERVMSD FYHQRFNLLV CTTIIETGID VPTANTIVIN RADHLGLAQL
HQLRGRVGRS HHQAYAYLLT PHPKRMTKDA VKRLEAIASL EDLGAGFTLA THDLEIRGAG
ELLGDEQSGQ IQSVGFTLFM EMLEQAVEAL KAGKEPSLDD LLREQTEVEL RLPALLPEDY
IPDINTRLSL YKRIASAGNE DALNEIKVEL IDRFGLLPDA ATNLLTLNML KLKAATLGLR
RIEAGEKGGY FEFTENAAID PGFLVGLLQS QPQQFRMDGP TKLKVTATLT DRRERIQYVD
GFLTQLAENT L
//
