ID A0A0F6AIS2_9GAMM Unreviewed; 224 AA.
AC A0A0F6AIS2;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=tRNA (guanine(46)-N(7))-methyltransferase {ECO:0000256|ARBA:ARBA00011977};
DE EC=2.1.1.33 {ECO:0000256|ARBA:ARBA00011977};
GN ORFNames=N479_25385 {ECO:0000313|EMBL:KKE85644.1};
OS Pseudoalteromonas luteoviolacea S4054.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC Pseudoalteromonadaceae; Pseudoalteromonas.
OX NCBI_TaxID=1129367 {ECO:0000313|EMBL:KKE85644.1, ECO:0000313|Proteomes:UP000033434};
RN [1] {ECO:0000313|EMBL:KKE85644.1, ECO:0000313|Proteomes:UP000033434}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S4054 {ECO:0000313|EMBL:KKE85644.1,
RC ECO:0000313|Proteomes:UP000033434};
RX PubMed=25879706; DOI=10.1186/s12864-015-1365-z;
RA Machado H., Sonnenschein E.C., Melchiorsen J., Gram L.;
RT "Genome mining reveals unlocked bioactive potential of marine Gram-negative
RT bacteria.";
RL BMC Genomics 16:158-158(2015).
CC -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at position 46
CC (m7G46) in tRNA. {ECO:0000256|ARBA:ARBA00003015}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-
CC methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269,
CC ChEBI:CHEBI:74480; EC=2.1.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000142};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKE85644.1}.
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DR EMBL; AUXW01000021; KKE85644.1; -; Genomic_DNA.
DR RefSeq; WP_046354193.1; NZ_AUXW01000021.1.
DR AlphaFoldDB; A0A0F6AIS2; -.
DR PATRIC; fig|1129367.4.peg.284; -.
DR Proteomes; UP000033434; Unassembled WGS sequence.
DR GO; GO:0008176; F:tRNA (guanine(46)-N7)-methyltransferase activity; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase_Trmb.
DR Pfam; PF02390; Methyltransf_4; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51625; SAM_MT_TRMB; 1.
PE 4: Predicted;
KW Transferase {ECO:0000313|EMBL:KKE85644.1}.
SQ SEQUENCE 224 AA; 25333 MW; C18A4CEA61C80671 CRC64;
MADANSRSIT TNQTGLHEKL DEIVLKHLNA EFKKPIAEHT LQAFNEVNEK VLAFSGPIIL
DSCCGVGEST ANLAQRHPEA LVIGIDKSSH RLDKHDVEYK QGEHGQYILV QADLNDFWRL
ACEADWQPSH HYLLYPNPWP KAKHIQRRWH GAAVFPFIVK LGGILEVRSN WSTYVEEFAR
ALHLAGVDAV CETYESEQAV TPFERKYWAS GQSSTRLVVS LSRS
//
