ID A0A0F6R0P2_9CORY Unreviewed; 646 AA.
AC A0A0F6R0P2;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=UL82_01675 {ECO:0000313|EMBL:AKE40563.1};
OS Corynebacterium kutscheri.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
OC Corynebacteriaceae; Corynebacterium.
OX NCBI_TaxID=35755 {ECO:0000313|EMBL:AKE40563.1, ECO:0000313|Proteomes:UP000033457};
RN [1] {ECO:0000313|EMBL:AKE40563.1, ECO:0000313|Proteomes:UP000033457}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20755 {ECO:0000313|EMBL:AKE40563.1,
RC ECO:0000313|Proteomes:UP000033457};
RX PubMed=26021937;
RA Ruckert C., Albersmeier A., Winkler A., Tauch A.;
RT "Complete Genome Sequence of Corynebacterium kutscheri DSM 20755, a
RT Corynebacterial Type Strain with Remarkably Low G+C Content of Chromosomal
RT DNA.";
RL Genome Announc. 3:e00571-15(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR EMBL; CP011312; AKE40563.1; -; Genomic_DNA.
DR RefSeq; WP_052735837.1; NZ_LR134381.1.
DR AlphaFoldDB; A0A0F6R0P2; -.
DR SMR; A0A0F6R0P2; -.
DR STRING; 35755.UL82_01675; -.
DR KEGG; cku:UL82_01675; -.
DR HOGENOM; CLU_004744_4_1_11; -.
DR OrthoDB; 9805159at2; -.
DR Proteomes; UP000033457; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000033457}.
FT DOMAIN 163..545
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 646 AA; 72782 MW; 6E90C60308C97262 CRC64;
MNLHSEEFNQ AFYFDGELGT QYQLQRTTFR LWAPTAEVVM LHDHTMKRSL ALSKIDPDQW
RGVWQLIIEG DCNGLEYTYE ILHDGGRITH TVDPYARAAT AHSQRAVVLA PEAPAKRIPG
LAMCDAIIWE GHIRDLTIAE NNGITNKGKF LGLAEQGTRT TAGNLSGLDY VLSLGVTHIQ
LLPIFDFGSV DETGDLSFNA QYNWGYDPVL YNVVEGSYAT DPVDPHIRIR ELKQLINTLH
EHGLRVIMDV VYNHVYDSAS NPLELTVPGY FFRMRDDGTY HDATACGNET ASEQLMMRKF
IVDSVVYWAR EFAIDGFRFD LMGIHDVETM NAVRSALDEI DPAIIIIGEG WRMGNHRSGV
EPADQQHGEL MPGIGMFNDC FRDVLKGDNF VLQGAGFVSK NPDFNHHDAK NFGDWAASYG
PEQAARAVYD NLLGAQSVRR YRDASQSVVY NEAHDNYTMF DKLAGSATLA GVSEQELARR
HALATTIQFL AAGTIFIHAG QELLRTKYGH ENSYNLPDQI NAFDYDRGQQ FPVNHELFKG
LVALRKKLAW LRETNYLKLA DRTSLERIGE HGGLTISYRI RDAFGAGRHA LIAMNASDWP
WTVFFEENHF ACYLHDMQVD LDPVEYVAAG EFVVQPLTVT LLELWD
//
