ID A0A0F8EEM7_9EURY Unreviewed; 323 AA.
AC A0A0F8EEM7;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Nitrogenase molybdenum-iron cofactor biosynthesis protein {ECO:0000313|EMBL:KKG13223.1};
GN ORFNames=EO92_15465 {ECO:0000313|EMBL:KKG13223.1};
OS Methanosarcina sp. 2.H.A.1B.4.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=1483600 {ECO:0000313|EMBL:KKG13223.1, ECO:0000313|Proteomes:UP000034052};
RN [1] {ECO:0000313|EMBL:KKG13223.1, ECO:0000313|Proteomes:UP000034052}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2.H.A.1B.4 {ECO:0000313|EMBL:KKG13223.1,
RC ECO:0000313|Proteomes:UP000034052};
RX PubMed=25756680;
RA Youngblut N.D., Wirth J.S., Henriksen J.R., Smith M., Simon H.,
RA Metcalf W.W., Whitaker R.J.;
RT "Genomic and phenotypic differentiation among Methanosarcina mazei
RT populations from Columbia River sediment.";
RL ISME J. 0:0-0(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKG13223.1}.
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DR EMBL; JJOV01000001; KKG13223.1; -; Genomic_DNA.
DR RefSeq; WP_048168623.1; NZ_JJOV01000001.1.
DR AlphaFoldDB; A0A0F8EEM7; -.
DR PATRIC; fig|1483600.3.peg.3412; -.
DR UniPathway; UPA00782; -.
DR Proteomes; UP000034052; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR000385; MoaA_NifB_PqqE_Fe-S-bd_CS.
DR InterPro; IPR005980; Nase_CF_NifB.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR01290; nifB; 1.
DR PANTHER; PTHR43787:SF13; FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB; 1.
DR PANTHER; PTHR43787; FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00281; FeMo_cofactor_biosynthesis_pro; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS01305; MOAA_NIFB_PQQE; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 36..278
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 323 AA; 36175 MW; B3A2FFDF325BF259 CRC64;
MPEKNQSIEE PNNGPILGEE LLRKIAEHPC YDKKAQHKYG RIHLAVAPKC NIQCNFCVRE
FDCVNESRPG VTSKVLTPEE ALEKTKQILA EYPFIKVVAI AGPGDPLAND ATFETFELIR
KEFPEVTLCM STNGLLLPEK LQDMLRVGVS TLTVTVNAID PEIQAKIVDH IVYHGEVYRG
VEAAEIQIKN QLQGIKAAVD AGIVVKVNTV LTPGINDKHV VEIARKLNEL GVYIMNVMPL
INQGAFADIE PPTAEERKAV QEACEPYVMQ MRHCRQCRAD AYGLLAQDMS QMSEERRKVI
KIQTKEDMEK AREILEKNGK EEA
//
