ID A0A0F9X7R4_TRIHA Unreviewed; 1059 AA.
AC A0A0F9X7R4;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=sn-1-specific diacylglycerol lipase {ECO:0000256|ARBA:ARBA00026104};
DE EC=3.1.1.116 {ECO:0000256|ARBA:ARBA00026104};
GN ORFNames=THAR02_11032 {ECO:0000313|EMBL:KKO96862.1};
OS Trichoderma harzianum (Hypocrea lixii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX NCBI_TaxID=5544 {ECO:0000313|EMBL:KKO96862.1, ECO:0000313|Proteomes:UP000034112};
RN [1] {ECO:0000313|Proteomes:UP000034112}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T6776 {ECO:0000313|Proteomes:UP000034112};
RX PubMed=26067977; DOI=10.1128/genomeA.00647-15;
RA Baroncelli R., Piaggeschi G., Fiorini L., Bertolini E., Zapparata A.,
RA Pe M.E., Sarrocco S., Vannacci G.;
RT "Draft whole-genome sequence of the biocontrol agent Trichoderma harzianum
RT T6776.";
RL Genome Announc. 3:E0064715-E0064715(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty
CC acid + H(+); Xref=Rhea:RHEA:33275, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:28868; EC=3.1.1.116;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33276;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKO96862.1}.
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DR EMBL; JOKZ01000706; KKO96862.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0F9X7R4; -.
DR OMA; SWKVHKG; -.
DR OrthoDB; 373802at2759; -.
DR Proteomes; UP000034112; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR CDD; cd00519; Lipase_3; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002921; Fungal_lipase-like.
DR PANTHER; PTHR45792; DIACYLGLYCEROL LIPASE HOMOLOG-RELATED; 1.
DR PANTHER; PTHR45792:SF7; PUTATIVE (AFU_ORTHOLOGUE AFUA_6G02710)-RELATED; 1.
DR Pfam; PF01764; Lipase_3; 2.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000034112};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 736..828
FT /note="Fungal lipase-like"
FT /evidence="ECO:0000259|Pfam:PF01764"
FT DOMAIN 857..902
FT /note="Fungal lipase-like"
FT /evidence="ECO:0000259|Pfam:PF01764"
FT REGION 238..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 373..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 474..517
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 542..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..419
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 420..450
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 492..514
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 542..559
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1059 AA; 115506 MW; 32EF651A4EDF32E1 CRC64;
MATDAVVASK QAQYKQGPTL LPSPVAQAVS LAARSTSLAI RIGSVVSNAS LDAARLTTLG
GLELARGLIE GVISRAAKDT LEQSRTELAI QDTETVLERS LENLHYAVTQ AVFWTSVSFR
LTGTTISTAS AGSQLLLSSL DQLFGSTDSS RAVASIITLV RREFENPATG LQGERVGVID
LTLALSTLAY LQRACRKTAQ EERRRQSYEE IIWDVVVLED GDRVDVDEKV FSEIRGGYNA
QDRDDIPSSP PTDPWTGAED DEAVVSRLKS QIIASLSPGT TVSVSNSVSS VQTITVDVHG
SRLLSLPTPP GAEIVETKSI TTGSHLSYSS LDKHDQSSAS SYRVVYKIHR SKKGTSSFTG
DEDYKDEAVE IITDNQSCSS EETLAPGTPT ITIPDDSSPP ASPTMLPSSA FALQSRPSSS
TETKRRHKTQ SHEFSKPTKS SSRKHNSDSA KAIASRSAVS SKEPIYLEDL EAMANQKRQR
TPVHSSKSHD SHKKRKGEAV VSSRSSDKKT GLRHVLRGSG PSISTIWSKE SGSMDYISGQ
EKPKLHIKSP HRDSAKELRA GSPTSDLVPR SPTEHHSVYL APPGHRRNRS YATSIYSVGT
NDSQSSLMLS SYYQKSSYNT DDALHALRQE GFVDGTFPEG HLLPNITRYM RFSSACYGSN
FLRLTGVTNE TPKLGVWDGV HNDIRHFVHH TESQSDNILL ASFVDPNGGS DATGATGSGV
PLVHYISLDH AAKAVVLACR GTLGFEDVLA DLTCDYDRLV WRGKGYRVHK GIHASARRLL
YGGDGRVLLT LKEALREFPD YGLVLCGHSL GAGVTSLLGI MLSEPNPIGP GFVTSAEPYT
LRPPPNEALV NVKLSDIRPP SGRRIHVYAY GPPAVMSSSL RKITRGLITT VVHGNDLVPH
LSLGLLHDFQ GVALAFKHDE NNTKSEIRQR IWNTLQDNVS ERWYSYRSAS KVASSGGVSD
EERWMLPALE NMRTSMKGKK LLPPGEVFTI ETQRVLRRDA FLLLDEEHIG RPAQRIVLKY
VKDVEGRFGE LRFGTSMLTD HSPAKYEDAL NKLRVGVSA
//
