ID A0A0G0A4Q2_TRIHA Unreviewed; 510 AA.
AC A0A0G0A4Q2;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=non-reducing end alpha-L-arabinofuranosidase {ECO:0000256|ARBA:ARBA00012670};
DE EC=3.2.1.55 {ECO:0000256|ARBA:ARBA00012670};
GN ORFNames=THAR02_00657 {ECO:0000313|EMBL:KKP07227.1};
OS Trichoderma harzianum (Hypocrea lixii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX NCBI_TaxID=5544 {ECO:0000313|EMBL:KKP07227.1, ECO:0000313|Proteomes:UP000034112};
RN [1] {ECO:0000313|Proteomes:UP000034112}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T6776 {ECO:0000313|Proteomes:UP000034112};
RX PubMed=26067977; DOI=10.1128/genomeA.00647-15;
RA Baroncelli R., Piaggeschi G., Fiorini L., Bertolini E., Zapparata A.,
RA Pe M.E., Sarrocco S., Vannacci G.;
RT "Draft whole-genome sequence of the biocontrol agent Trichoderma harzianum
RT T6776.";
RL Genome Announc. 3:E0064715-E0064715(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside
CC residues in alpha-L-arabinosides.; EC=3.2.1.55;
CC Evidence={ECO:0000256|ARBA:ARBA00001462};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 54 family.
CC {ECO:0000256|ARBA:ARBA00006963}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKP07227.1}.
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DR EMBL; JOKZ01000010; KKP07227.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G0A4Q2; -.
DR OMA; THYNTGC; -.
DR OrthoDB; 2573673at2759; -.
DR Proteomes; UP000034112; Unassembled WGS sequence.
DR GO; GO:0046556; F:alpha-L-arabinofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0031221; P:arabinan metabolic process; IEA:InterPro.
DR GO; GO:0046373; P:L-arabinose metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.200; -; 1.
DR Gene3D; 2.80.10.50; -; 1.
DR InterPro; IPR015289; A-L-arabinofuranosidase_B_cat.
DR InterPro; IPR038964; ABFB.
DR InterPro; IPR007934; AbfB_ABD.
DR InterPro; IPR036195; AbfB_ABD_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR PANTHER; PTHR39447; ALPHA-L-ARABINOFURANOSIDASE B; 1.
DR PANTHER; PTHR39447:SF2; ALPHA-L-ARABINOFURANOSIDASE B; 1.
DR Pfam; PF05270; AbfB; 1.
DR Pfam; PF09206; ArabFuran-catal; 1.
DR SUPFAM; SSF110221; AbfB domain; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR638964-3};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000034112};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 27..510
FT /note="non-reducing end alpha-L-arabinofuranosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5032505046"
FT DOMAIN 28..343
FT /note="Alpha-L-arabinofuranosidase B catalytic"
FT /evidence="ECO:0000259|Pfam:PF09206"
FT DOMAIN 364..505
FT /note="Alpha-L-arabinofuranosidase B arabinose-binding"
FT /evidence="ECO:0000259|Pfam:PF05270"
FT ACT_SITE 232
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR638964-1"
FT ACT_SITE 307
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR638964-1"
FT DISULFID 29..39
FT /evidence="ECO:0000256|PIRSR:PIRSR638964-3"
FT DISULFID 89..94
FT /evidence="ECO:0000256|PIRSR:PIRSR638964-3"
FT DISULFID 185..186
FT /evidence="ECO:0000256|PIRSR:PIRSR638964-3"
FT DISULFID 413..451
FT /evidence="ECO:0000256|PIRSR:PIRSR638964-3"
SQ SEQUENCE 510 AA; 52710 MW; 63B742D5BE96CE4B CRC64;
MFSRVNRRRS SVLTFGLVAT GSLVTAGPCD IYASGGTPCV AAHSTTRALY GAYSGSLYQV
SRGSDGATTN IAPLSAGGVA NAAAQDSFCA GTTCLITTIY DQSGHGNHLT QAPPGGAASG
PLPGGFDNLA SAFAAPVRLN GQKAYGVFIA PTTGYRNNAA NGTATGDGSE GMYAIFDGTH
YNTACCFDYG NAETNSRDTG NGHMEAIYFG TGDGSGRGTG SGSGPWIMAD LENGLFSGLS
PINNPADPTI NFRFVTAIIK GEPNQWAIRG GDATSGTLST FYSGARPSGG YNPMSKEGAI
ILGTGGDNSD GAQGTFYEGV MTSGYPSDST EASVQANIVA AKYVYDTSLM TSGPALTVGS
SISLRATTSC CTTRYIAHTG ATVNTQVVSS SSATALKQQA SWTVRTGLGN SACFSFESVD
TPGSFIRHSN FQLMLNANDG SKLFSEDATF CPQAGLNGQG NSFRSWSYPT RYWRHFNALL
YIAANGGEHD FDSTTLFNDD VSFVVSASFA
//
