ID A0A0G0DE04_9BACT Unreviewed; 544 AA.
AC A0A0G0DE04;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 13-SEP-2023, entry version 31.
DE RecName: Full=CTP synthase (glutamine hydrolyzing) {ECO:0000256|ARBA:ARBA00012291};
DE EC=6.3.4.2 {ECO:0000256|ARBA:ARBA00012291};
GN ORFNames=UR56_C0012G0002 {ECO:0000313|EMBL:KKP61500.1};
OS Candidatus Roizmanbacteria bacterium GW2011_GWC2_34_23.
OC Bacteria; Candidatus Roizmanbacteria.
OX NCBI_TaxID=1618484 {ECO:0000313|EMBL:KKP61500.1, ECO:0000313|Proteomes:UP000034004};
RN [1] {ECO:0000313|EMBL:KKP61500.1, ECO:0000313|Proteomes:UP000034004}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Brown C.T., Hug L.A., Thomas B.C., Sharon I., Castelle C.J., Singh A.,
RA Wilkins M.J., Williams K.H., Banfield J.F.;
RT "rRNA introns, odd ribosomes, and small enigmatic genomes across a large
RT radiation of phyla.";
RL Nature 0:0-0(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-
CC glutamate + phosphate; Xref=Rhea:RHEA:26426, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37563, ChEBI:CHEBI:43474, ChEBI:CHEBI:46398,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.4.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000314};
CC -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo pathway;
CC CTP from UDP: step 2/2. {ECO:0000256|ARBA:ARBA00005171}.
CC -!- SIMILARITY: Belongs to the CTP synthase family.
CC {ECO:0000256|ARBA:ARBA00007533}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKP61500.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LBPR01000012; KKP61500.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G0DE04; -.
DR STRING; 1618484.UR56_C0012G0002; -.
DR PATRIC; fig|1618484.3.peg.462; -.
DR UniPathway; UPA00159; UER00277.
DR Proteomes; UP000034004; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003883; F:CTP synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0044210; P:'de novo' CTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd01746; GATase1_CTP_Synthase; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR004468; CTP_synthase.
DR InterPro; IPR017456; CTP_synthase_N.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR033828; GATase1_CTP_Synthase.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00337; PyrG; 1.
DR PANTHER; PTHR11550; CTP SYNTHASE; 1.
DR PANTHER; PTHR11550:SF0; CTP SYNTHASE-RELATED; 1.
DR Pfam; PF06418; CTP_synth_N; 1.
DR Pfam; PF00117; GATase; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962,
KW ECO:0000256|PROSITE-ProRule:PRU00605};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Pyrimidine biosynthesis {ECO:0000256|ARBA:ARBA00022975}.
FT DOMAIN 2..265
FT /note="CTP synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF06418"
FT DOMAIN 307..535
FT /note="Glutamine amidotransferase"
FT /evidence="ECO:0000259|Pfam:PF00117"
FT ACT_SITE 385
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 516
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 518
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
SQ SEQUENCE 544 AA; 61537 MW; 67DE51877AE565D5 CRC64;
MKYIFISGGV ISGIGKGITA ASISLLLKQA GYRVAPLKFE NYLNLDAGTI NPIEHGDVFL
CEDGTEADMD IGTYEKFLNE DMGKDNFVTI GEIYKTVIDR ERRFEYKGED VEAIPHITNE
IIDRINLLGK KKKADIVVIE LGGTAGEYQN IFYYEASRIL TLKNPGDVIH VHVSYVPTPN
HLGEPKTKPT QLSVRTLNSM GIQPDFIVAR SEKLLDKRRI ERFALFCNVQ PENIISNPDV
SHPYEVPLIL EKQQIAEKIL NKFKLKVVEP DLTDWKKLIE KIYSKKSGKV INIAIVGKYF
GTGDYQLRDS YAALFDALNH GAFEVGAEVK THWIDAEKVE KHGVEIIGKP DGLIVPIGWG
ERGAEGMISA ASYARKNNIP YLGLCYGMQL AVIAFARDIL GWPDANTNEN NSKTKHPVIH
LMPAQKKYMD KRSYGGTMRL GNWDAKIKKG TKAFQIYGKE ETSERHRHRY EFNDEFKQDF
EKNGLIISAR SNIENLAEII ELPESTHPFY FGTQGHPEYK SRPLQPHPIF VAFLRACEKM
TNHK
//
