ID A0A0G0PRN8_9BACT Unreviewed; 567 AA.
AC A0A0G0PRN8;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 13-SEP-2023, entry version 32.
DE RecName: Full=CTP synthase (glutamine hydrolyzing) {ECO:0000256|ARBA:ARBA00012291};
DE EC=6.3.4.2 {ECO:0000256|ARBA:ARBA00012291};
GN ORFNames=UT61_C0067G0002 {ECO:0000313|EMBL:KKR27756.1};
OS Candidatus Woesebacteria bacterium GW2011_GWA1_39_8.
OC Bacteria; Candidatus Woesebacteria.
OX NCBI_TaxID=1618552 {ECO:0000313|EMBL:KKR27756.1, ECO:0000313|Proteomes:UP000034793};
RN [1] {ECO:0000313|EMBL:KKR27756.1, ECO:0000313|Proteomes:UP000034793}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Brown C.T., Hug L.A., Thomas B.C., Sharon I., Castelle C.J., Singh A.,
RA Wilkins M.J., Williams K.H., Banfield J.F.;
RT "rRNA introns, odd ribosomes, and small enigmatic genomes across a large
RT radiation of phyla.";
RL Nature 0:0-0(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-
CC glutamate + phosphate; Xref=Rhea:RHEA:26426, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37563, ChEBI:CHEBI:43474, ChEBI:CHEBI:46398,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.4.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000314};
CC -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo pathway;
CC CTP from UDP: step 2/2. {ECO:0000256|ARBA:ARBA00005171}.
CC -!- SIMILARITY: Belongs to the CTP synthase family.
CC {ECO:0000256|ARBA:ARBA00007533}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKR27756.1}.
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DR EMBL; LBXL01000067; KKR27756.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G0PRN8; -.
DR PATRIC; fig|1618552.3.peg.1218; -.
DR UniPathway; UPA00159; UER00277.
DR Proteomes; UP000034793; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003883; F:CTP synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0044210; P:'de novo' CTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd01746; GATase1_CTP_Synthase; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR004468; CTP_synthase.
DR InterPro; IPR017456; CTP_synthase_N.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR033828; GATase1_CTP_Synthase.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00337; PyrG; 1.
DR PANTHER; PTHR11550; CTP SYNTHASE; 1.
DR PANTHER; PTHR11550:SF0; CTP SYNTHASE-RELATED; 1.
DR Pfam; PF06418; CTP_synth_N; 1.
DR Pfam; PF00117; GATase; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962,
KW ECO:0000256|PROSITE-ProRule:PRU00605};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Pyrimidine biosynthesis {ECO:0000256|ARBA:ARBA00022975}.
FT DOMAIN 2..265
FT /note="CTP synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF06418"
FT DOMAIN 306..561
FT /note="Glutamine amidotransferase"
FT /evidence="ECO:0000259|Pfam:PF00117"
FT ACT_SITE 388
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 542
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 544
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
SQ SEQUENCE 567 AA; 63046 MW; 86D116E4D6DC4F9E CRC64;
MKFIIVSGGV LSGLGKGVTA SSIGLLLKSR GVKVTAMKCD MYLNIDAGTM NPIEHGEVFV
THDGVETDQD LGHYERFLGQ TLTRANYLTN GQIYKYVLDR ERALEYGGAC VEPYHHIPPE
IIERWYAAGE ASKADIVIIE LGGTVGEYEG MLFFEAVRRL KLLKPKDIVL VHVGYLPVPP
SIGELKSKPL QQSVGFLNSL GLSPDIIIGR AEKPVDDKRK EKIALASGLL KEDVISNPDV
ESIYQVPIVL EEQRFSQRLI AKLGLRAAKT PNLTSWKTML KKAKESKKEV SIAVIGKYQK
TGDYTLSDSY VCVVEALKHA GWLLGVQPNL IWVDSEGLEG KTSSGVASIL SKVDAVLVPQ
GWGKRGVEGK IKAAKYAREN KVPYLGLCFG MQMATIEFAR HVIGLTAANS EEIDPKTKYP
VIHIMKNQRK YLANKQYGGT IRLGAWPCRI NKDTVLEKAY KKYGGDITSP WYTKNPAKDF
KIEAKLARVV FERHRHRYEF NNAFTIKFEE NGFIISGSSP DGLLVEAVEL ANHPFFVGTQ
YHPEYESRPL TPHPIFCAFI EAAKKSR
//
