ID A0A0G0VK66_9BACT Unreviewed; 478 AA.
AC A0A0G0VK66;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Cobalamin-binding radical SAM protein {ECO:0000313|EMBL:KKS00017.1};
GN ORFNames=UU49_C0001G0017 {ECO:0000313|EMBL:KKS00017.1};
OS Candidatus Magasanikbacteria bacterium GW2011_GWC2_41_17.
OC Bacteria; Candidatus Magasanikbacteria.
OX NCBI_TaxID=1619048 {ECO:0000313|EMBL:KKS00017.1, ECO:0000313|Proteomes:UP000034108};
RN [1] {ECO:0000313|EMBL:KKS00017.1, ECO:0000313|Proteomes:UP000034108}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Brown C.T., Hug L.A., Thomas B.C., Sharon I., Castelle C.J., Singh A.,
RA Wilkins M.J., Williams K.H., Banfield J.F.;
RT "rRNA introns, odd ribosomes, and small enigmatic genomes across a large
RT radiation of phyla.";
RL Nature 0:0-0(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKS00017.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LCAV01000001; KKS00017.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G0VK66; -.
DR STRING; 1619048.UU49_C0001G0017; -.
DR PATRIC; fig|1619048.3.peg.17; -.
DR Proteomes; UP000034108; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 10..145
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 196..428
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 478 AA; 55056 MW; 8CD8424D1C5C729A CRC64;
MSPFFCKKNK VLFIIHDLYQ DDNRFPLGPA YLAAVLDKNG AQVEVYCMDV FHYTNEQLAQ
HLQNNHYDLI GLGFSAARFV ETVLELCRVI NQNKKDAWLV LGGHGPSPIP EYILKATGVD
IVAIGEAENT IIDLLNCKIN HGDLSKVNGI AYLENERFKV TAPNRPIIKL DELPFPLWQI
FPMKIYVECN KMFRQSPGEK SMSLVSSRGC INRCNFCYRM EKGIRLRSVD SIIGELKILY
HQYGVNNYFF EDELFVLNKS RLLKFEKGLI ENGLKIKFFC NARVDIMDED TVEVLKRCGC
QFIGFGFESS NDHVLKLMNK NATVEQNLRA LEVVKKVGGI GMGLNFIWNN LGDDEETLKR
NVELIKKYNT FYQCRTIRPV TPYPGSDLYY QLIKMGKIKG PEDFFDKYYN SDLIFKNLMN
MSDEKAYELL LEANTELIRD HFNHVGDSKG EAEKLIKQFA DLYSGKSNKF RGSRHYTK
//
