ID A0A0G0YL69_9BACT Unreviewed; 381 AA.
AC A0A0G0YL69;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=tRNA-uridine 2-sulfurtransferase {ECO:0000256|ARBA:ARBA00011949};
DE EC=2.8.1.13 {ECO:0000256|ARBA:ARBA00011949};
GN ORFNames=UV01_C0010G0006 {ECO:0000313|EMBL:KKS37430.1};
OS Parcubacteria group bacterium GW2011_GWA2_42_14.
OC Bacteria.
OX NCBI_TaxID=1618820 {ECO:0000313|EMBL:KKS37430.1, ECO:0000313|Proteomes:UP000034025};
RN [1] {ECO:0000313|EMBL:KKS37430.1, ECO:0000313|Proteomes:UP000034025}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Brown C.T., Hug L.A., Thomas B.C., Sharon I., Castelle C.J., Singh A.,
RA Wilkins M.J., Williams K.H., Banfield J.F.;
RT "rRNA introns, odd ribosomes, and small enigmatic genomes across a large
RT radiation of phyla.";
RL Nature 0:0-0(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in
CC tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-
CC cysteinyl-[protein]; Xref=Rhea:RHEA:47032, Rhea:RHEA-COMP:10131,
CC Rhea:RHEA-COMP:11726, Rhea:RHEA-COMP:11727, Rhea:RHEA-COMP:11728,
CC ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:61963, ChEBI:CHEBI:65315, ChEBI:CHEBI:87170,
CC ChEBI:CHEBI:456215; EC=2.8.1.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001042};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKS37430.1}.
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DR EMBL; LCCV01000010; KKS37430.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G0YL69; -.
DR STRING; 1618820.UV01_C0010G0006; -.
DR PATRIC; fig|1618820.3.peg.969; -.
DR Proteomes; UP000034025; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR CDD; cd01998; tRNA_Me_trans; 1.
DR Gene3D; 2.30.30.280; Adenine nucleotide alpha hydrolases-like domains; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR046885; MnmA-like_C.
DR InterPro; IPR046884; MnmA-like_central.
DR InterPro; IPR023382; MnmA-like_central_sf.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR004506; tRNA-specific_2-thiouridylase.
DR NCBIfam; TIGR00420; trmU; 1.
DR PANTHER; PTHR11933:SF5; MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1; 1.
DR PANTHER; PTHR11933; TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE; 1.
DR Pfam; PF03054; tRNA_Me_trans; 1.
DR Pfam; PF20258; tRNA_Me_trans_C; 1.
DR Pfam; PF20259; tRNA_Me_trans_M; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 240..297
FT /note="tRNA-specific 2-thiouridylase MnmA-like central"
FT /evidence="ECO:0000259|Pfam:PF20259"
FT DOMAIN 306..379
FT /note="tRNA-specific 2-thiouridylase MnmA-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20258"
SQ SEQUENCE 381 AA; 42102 MW; 56A9D49CFC0F51F0 CRC64;
MPETVQKKDS PLSKTTKNVV FLMLSGGKDS GVAARLLKDE GKEVIGFFIK GWAPTGLKCL
EPPEMRDAAE IARVVGIEFF PILNWSEHFY NAVFDPMLSG YLSGITPNPD TLCNREIKFG
LFAKFAFGRG ADYIASGHYV RKNDNPLQLL VARDERKDQS YFLYDLKTDI LERSLFPIGN
YVKSEEVIPM ARSFGLPQRV IEKRPTRDIC FLLKSEEDAT DGGRIGMEKL LRLEGEKRGI
KFSSGPVIHE KTGKVVGEHN GVSLFAVTIG QRHGLGLAGG KLLYVSRKDI LKNAFYVNSE
KPSSPEVVVK NLNWISGAPD FGKKLSAKIR TPQKAQSCRV EKIGEDEIRV KFDTPQNSVA
PGQACVLYDG EVVLGGGIIV S
//
