ID A0A0G1LRG8_9BACT Unreviewed; 863 AA.
AC A0A0G1LRG8;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=Replicative DNA helicase {ECO:0000256|ARBA:ARBA00021957, ECO:0000256|RuleBase:RU362085};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU362085};
GN ORFNames=UX00_C0003G0038 {ECO:0000313|EMBL:KKT98366.1};
OS Microgenomates group bacterium GW2011_GWB1_45_17.
OC Bacteria.
OX NCBI_TaxID=1618506 {ECO:0000313|EMBL:KKT98366.1, ECO:0000313|Proteomes:UP000034461};
RN [1] {ECO:0000313|EMBL:KKT98366.1, ECO:0000313|Proteomes:UP000034461}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Brown C.T., Hug L.A., Thomas B.C., Sharon I., Castelle C.J., Singh A.,
RA Wilkins M.J., Williams K.H., Banfield J.F.;
RT "rRNA introns, odd ribosomes, and small enigmatic genomes across a large
RT radiation of phyla.";
RL Nature 0:0-0(2015).
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity.
CC {ECO:0000256|ARBA:ARBA00002835, ECO:0000256|RuleBase:RU362085}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU362085};
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000256|ARBA:ARBA00008428, ECO:0000256|RuleBase:RU362085}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKT98366.1}.
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DR EMBL; LCKN01000003; KKT98366.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G1LRG8; -.
DR PATRIC; fig|1618506.3.peg.176; -.
DR Proteomes; UP000034461; Unassembled WGS sequence.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR CDD; cd00984; DnaB_C; 1.
DR CDD; cd00081; Hint; 2.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00665; DnaB; 1.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR PANTHER; PTHR30153:SF2; REPLICATIVE DNA HELICASE; 1.
DR PANTHER; PTHR30153; REPLICATIVE DNA HELICASE DNAB; 1.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 2.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF48024; N-terminal domain of DnaB helicase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS51199; SF4_HELICASE; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU362085};
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU362085};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362085};
KW Helicase {ECO:0000256|RuleBase:RU362085, ECO:0000313|EMBL:KKT98366.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362085};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU362085};
KW Primosome {ECO:0000256|ARBA:ARBA00022515, ECO:0000256|RuleBase:RU362085};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 176..379
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
FT DOMAIN 494..644
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 786..808
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT DOMAIN 808..863
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
SQ SEQUENCE 863 AA; 97295 MW; 0C786EB8B01C8CDD CRC64;
MADPKLPPHN IQAEESVLGA MLIDRDAIVQ IAEFLEPKHF YREAHRDIYD GMLKLFADRE
PIDVVTLTDM LKKTGRLKDI GGTAYLADLA AKVPTAAHVK QYAKIIKDMA IKRFLITLAS
DLSEMALNPA RETSDILDTA EQNIFGLSQK HMDRSFISLK TALAESFDRL DELHKNSSGL
RGVPTGFAEL DDTLAGMQKS NLLILAARPG IGKTAFSLSI AKHVAVERKL NVGYFALEMS
KEEMVDRLLV QQADIDAWKL KTGRLDEEDF EKLSDAMGEL AEAPLFFDDT PGQSILEVRT
KARRLMAEHG LDLIIVDYLQ LMRGYTTDNR VQEVSEISQG LKNLARELKV PVLALSQLNR
SVESRGVKQP QLSDLRESGC LLGDTLIFRS DTGKRVPIVS LVGKKNIPVL AMDETMIIHQ
AVMSKVFPSG KKMVYEMQLR SGKRITASAN HPFFTVSGWK RLDQLKVGEQ LGTPRLLEIS
KELQQTLTDD QLIVLAHLIG DGCYVKRQPL HYTNSELPLI QYVAESAVHA FGIIPRLVRQ
EQWYHLYLSS PYKLARGKRN PIVRWLDEDL GIYGQHSRVK HIPTAVFSQP NATIALFLRH
LWSTDGCIAI RKEKKKGPLV SVYYASGSQR LAEDVAHLLL RLGVFSTVRE VKQHGYQAMW
NVAVQGAQAQ RHFLEIVGCV GEKEGRARRA LLLLQTVVSN PNLDVIPKAL WKYIEASRAE
FGLTTRAFHQ TMGWAYSGTG RQRNSIGRER LWNIAEALDN DLLRMFASSD VYWDEILSIT
KIGIRKVFDA TVPGYANFIA NDIVVHNSIE QDSDVVMFLY REEDEAPEHV KLTVAKHRNG
PLRTIDMFFK GDRIKFYGME QKR
//
