ID A0A0G3CG18_9GAMM Unreviewed; 612 AA.
AC A0A0G3CG18;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Glutathione-regulated potassium-efflux system protein KefB {ECO:0000256|HAMAP-Rule:MF_01412};
DE AltName: Full=K(+)/H(+) antiporter {ECO:0000256|HAMAP-Rule:MF_01412};
GN Name=kefB {ECO:0000256|HAMAP-Rule:MF_01412};
GN ORFNames=QQ39_01540 {ECO:0000313|EMBL:AKJ40926.1};
OS Pragia fontium.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Budviciaceae; Pragia.
OX NCBI_TaxID=82985 {ECO:0000313|EMBL:AKJ40926.1, ECO:0000313|Proteomes:UP000068021};
RN [1] {ECO:0000313|EMBL:AKJ40926.1, ECO:0000313|Proteomes:UP000068021}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=24613 {ECO:0000313|EMBL:AKJ40926.1,
RC ECO:0000313|Proteomes:UP000068021};
RX PubMed=26159528;
RA Snopkova K., Sedlar K., Bosak J., Chaloupkova E., Provaznik I., Smajs D.;
RT "Complete Genome Sequence of Pragia fontium 24613, an Environmental
RT Bacterium from the Family Enterobacteriaceae.";
RL Genome Announc. 3:e00740-15(2015).
CC -!- FUNCTION: Pore-forming subunit of a potassium efflux system that
CC confers protection against electrophiles. Catalyzes K(+)/H(+) antiport.
CC {ECO:0000256|HAMAP-Rule:MF_01412}.
CC -!- SUBUNIT: Interacts with the regulatory subunit KefG.
CC {ECO:0000256|HAMAP-Rule:MF_01412}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_01412}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_01412}. Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-pass
CC membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. KefB subfamily. {ECO:0000256|HAMAP-
CC Rule:MF_01412}.
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DR EMBL; CP010423; AKJ40926.1; -; Genomic_DNA.
DR RefSeq; WP_047779677.1; NZ_CP010423.1.
DR AlphaFoldDB; A0A0G3CG18; -.
DR STRING; 82985.QQ39_01540; -.
DR KEGG; pfq:QQ39_01540; -.
DR PATRIC; fig|82985.3.peg.327; -.
DR OrthoDB; 9781411at2; -.
DR Proteomes; UP000068021; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015503; F:glutathione-regulated potassium exporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:1902600; P:proton transmembrane transport; IEA:InterPro.
DR Gene3D; 1.20.1530.20; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR HAMAP; MF_01412; K_H_efflux_KefB; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR004771; K/H_exchanger.
DR InterPro; IPR020884; K_H_efflux_KefB.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR003148; RCK_N.
DR NCBIfam; TIGR00932; 2a37; 1.
DR PANTHER; PTHR46157:SF11; GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM PROTEIN KEFB; 1.
DR PANTHER; PTHR46157; K(+) EFFLUX ANTIPORTER 3, CHLOROPLASTIC; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR Pfam; PF02254; TrkA_N; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS51201; RCK_N; 1.
PE 3: Inferred from homology;
KW Antiport {ECO:0000256|ARBA:ARBA00022449, ECO:0000256|HAMAP-Rule:MF_01412};
KW Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01412};
KW Cell membrane {ECO:0000256|HAMAP-Rule:MF_01412};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065, ECO:0000256|HAMAP-
KW Rule:MF_01412};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_01412};
KW Potassium {ECO:0000256|ARBA:ARBA00022958, ECO:0000256|HAMAP-Rule:MF_01412};
KW Potassium transport {ECO:0000256|ARBA:ARBA00022538, ECO:0000256|HAMAP-
KW Rule:MF_01412}; Reference proteome {ECO:0000313|Proteomes:UP000068021};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP-
KW Rule:MF_01412};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP-
KW Rule:MF_01412};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|HAMAP-Rule:MF_01412}.
FT TRANSMEM 6..24
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 31..50
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 56..75
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 87..109
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 147..170
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 182..200
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 212..230
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 293..311
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 323..342
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 354..375
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT DOMAIN 401..522
FT /note="RCK N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51201"
FT COILED 565..592
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 612 AA; 67397 MW; 4563B2B68CF2227A CRC64;
METSPQLAAV LIFLSVAVLA VPLARRVGIG AVLGYLIAGI AIGPWGFRLI NDVDEILHFS
EMGVVLLLFV IGLELNPAKL WKMRKPIFGL GTAQVLFTTA IICGLLYWAG FGLPAAIIGA
LGLSMSSTAM ALQLMKEKGM RGDGGQSGFA VLLFQDMAVV PALALVPILA GKSTGDADWF
TVLSKVVGLA ILVLACRYLL RPIFRYVASS GVREVFTATA LLVVLGAAAL MQEIGFSMAL
GTFLAGVLLA ESEYQHELEV AIEPFKGLLL GLFFISVGMV LNLGVFYSHF LQVLIWVCVL
LLVKGSVLYG LARFFRINRS ERLQFSAVLS QGGEFAFVLF AAARAQNIFD TEQISLLLVV
VTISMMTTPF MMQIVDRILA RRYNSGEEND QKSDVEDDEP QVIVVGFGRF GQVVGRLLMA
NEIRLTVLER DVSQVGILRS HGYKVYYGDA TELDLLRAAG AAKAKAIVIA SDLPEETMTI
VHLCQQYFPN LSILARARGR VEAHELLSAG VELFSRETFS SATDLARKAL IDLGMHPHQA
YRAQQYFKRL DESMLRELVP VRQGNMQQIS RVAEARRELE NLFQREMQYD SKLSDDWDLD
EEVQETKHDT LD
//
