ID A0A0G3H7K8_9CORY Unreviewed; 697 AA.
AC A0A0G3H7K8;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=formate C-acetyltransferase {ECO:0000256|ARBA:ARBA00013214};
DE EC=2.3.1.54 {ECO:0000256|ARBA:ARBA00013214};
GN Name=pflB {ECO:0000313|EMBL:AKK08735.1};
GN ORFNames=CTEST_06485 {ECO:0000313|EMBL:AKK08735.1};
OS Corynebacterium testudinoris.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
OC Corynebacteriaceae; Corynebacterium.
OX NCBI_TaxID=136857 {ECO:0000313|EMBL:AKK08735.1, ECO:0000313|Proteomes:UP000035540};
RN [1] {ECO:0000313|EMBL:AKK08735.1, ECO:0000313|Proteomes:UP000035540}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44614 {ECO:0000313|EMBL:AKK08735.1,
RC ECO:0000313|Proteomes:UP000035540};
RX PubMed=26227591;
RA Ruckert C., Kriete M., Jaenicke S., Winkler A., Tauch A.;
RT "Complete Genome Sequence of the Type Strain Corynebacterium testudinoris
RT DSM 44614, Recovered from Necrotic Lesions in the Mouth of a Tortoise.";
RL Genome Announc. 3:e00784-15(2015).
RN [2] {ECO:0000313|Proteomes:UP000035540}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44614 {ECO:0000313|Proteomes:UP000035540};
RA Ruckert C., Albersmeier A., Winkler A., Tauch A.;
RT "Complete genome sequence of Corynebacterium testudinoris DSM 44614,
RT recovered from necrotic lesions in the mouth of a tortoise.";
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + formate = CoA + pyruvate; Xref=Rhea:RHEA:11844,
CC ChEBI:CHEBI:15361, ChEBI:CHEBI:15740, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.1.54;
CC Evidence={ECO:0000256|ARBA:ARBA00001179};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycyl radical enzyme (GRE) family. PFL
CC subfamily. {ECO:0000256|ARBA:ARBA00008375}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00493}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP011545; AKK08735.1; -; Genomic_DNA.
DR RefSeq; WP_047253046.1; NZ_JAASJB010000002.1.
DR AlphaFoldDB; A0A0G3H7K8; -.
DR STRING; 136857.CTEST_06485; -.
DR KEGG; cted:CTEST_06485; -.
DR PATRIC; fig|136857.5.peg.1288; -.
DR OrthoDB; 9803969at2; -.
DR Proteomes; UP000035540; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008861; F:formate C-acetyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.20.70.20; -; 1.
DR InterPro; IPR001150; Gly_radical.
DR InterPro; IPR004184; PFL_dom.
DR PANTHER; PTHR30191; FORMATE ACETYLTRANSFERASE; 1.
DR PANTHER; PTHR30191:SF0; FORMATE ACETYLTRANSFERASE 1; 1.
DR Pfam; PF01228; Gly_radical; 1.
DR Pfam; PF02901; PFL-like; 1.
DR PIRSF; PIRSF000379; For_Ac_trans_1; 1.
DR SUPFAM; SSF51998; PFL-like glycyl radical enzymes; 1.
DR PROSITE; PS51149; GLY_RADICAL_2; 1.
DR PROSITE; PS51554; PFL; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000313|EMBL:AKK08735.1}; Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Lyase {ECO:0000313|EMBL:AKK08735.1};
KW Organic radical {ECO:0000256|ARBA:ARBA00022818};
KW Pyruvate {ECO:0000313|EMBL:AKK08735.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000035540};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AKK08735.1}.
FT DOMAIN 6..623
FT /note="PFL"
FT /evidence="ECO:0000259|PROSITE:PS51554"
FT DOMAIN 630..697
FT /note="Glycine radical"
FT /evidence="ECO:0000259|PROSITE:PS51149"
FT ACT_SITE 414
FT /note="S-acetylcysteine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000379-1"
FT ACT_SITE 415
FT /note="Cysteine radical intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000379-1"
SQ SEQUENCE 697 AA; 77267 MW; 757B807FFD688AFE CRC64;
MTTVTAPDTQ AWEGFHEGPW QEAIDVRDFI SRNFTPYSGD AEFLSGPTEK TLRTWKHLED
NYLSVERQRR VYDVDTSTPA DIDAFAPGFI SDDDNVIVGL QTDTPLKRAM MPYGGWRMVE
TAIREANLEP NEDIKKIFTR YRKTHNEAVF DVYTPRIRAA RSSHIITGLP DAYGRGRIIG
DYRRVALYGV DKLIAEKEAS KHAAGPQGFS EHWARFREEH SEQIKALKKL KTMAASYGFD
ISGPAATAQE AVQWTYFGYL GAVKSQDGAA MSIGRLSSFF DIYFERDLAN GTITEEDAQE
IIDSLVLKLR IVRFLRTIDY DQIFSGDPYW ATWSDAGFMA DGRPQVTKTS FRLLQTLRNL
GPAPEPNITV FWDPQLPAGY KDFCAAISIE TSSIQYESDK QIRDRWGDDS AIACCVSPMA
VGKQMQFFGA RVNAAKSLLY AINGGRDEVT GKQITTEGEF SPIEGDGPLD FDEVWNKYEE
MLNWVIGTYV EALNIIHWSH DKYAYEAIEM ALHDSDIVRS MGCGIAGLSI VADSLSAIKY
AKVTPVRDDT GLIVDFITEG DFPVYGNDDD RADDIAATIV HTVMAKIKEI PMYRDAIPTQ
SVLTITSNVV YGKATGNFPS GHRAGTPFAP GANPENGADN HGMVASMLSV GKLDYHDALD
GISLTNTITP SGLGRTKDEQ VSNLVGVLDA GFIMDEN
//
