ID A0A0G3I9Q7_LIBAF Unreviewed; 360 AA.
AC A0A0G3I9Q7;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 13-SEP-2023, entry version 31.
DE RecName: Full=S-adenosylmethionine:tRNA ribosyltransferase-isomerase {ECO:0000256|HAMAP-Rule:MF_00113};
DE EC=2.4.99.17 {ECO:0000256|HAMAP-Rule:MF_00113};
DE AltName: Full=Queuosine biosynthesis protein QueA {ECO:0000256|HAMAP-Rule:MF_00113};
GN Name=queA {ECO:0000256|HAMAP-Rule:MF_00113};
GN ORFNames=G293_04560 {ECO:0000313|EMBL:AKK20527.1};
OS Candidatus Liberibacter africanus PTSAPSY.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Liberibacter.
OX NCBI_TaxID=1277257 {ECO:0000313|EMBL:AKK20527.1, ECO:0000313|Proteomes:UP000035503};
RN [1] {ECO:0000313|Proteomes:UP000035503}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PTSAPSY {ECO:0000313|Proteomes:UP000035503};
RA Lin H., Pietersen G., Sahota P., Lou B., Han S., Read D.A., Civerolo E.L.,
RA Gupta G.;
RT "Complete genome sequence of 'Candidatus Liberibacter africanus, bacterium
RT associated with citrus huanglongbing'.";
RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AKK20527.1, ECO:0000313|Proteomes:UP000035503}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PTSAPSY {ECO:0000313|EMBL:AKK20527.1,
RC ECO:0000313|Proteomes:UP000035503};
RX PubMed=26184931;
RA Lin H., Pietersen G., Han C., Read D.A., Lou B., Gupta G., Civerolo E.L.;
RT "Complete Genome Sequence of 'Candidatus Liberibacter africanus,' a
RT Bacterium Associated with Citrus Huanglongbing.";
RL Genome Announc. 3:e00733-15(2015).
CC -!- FUNCTION: Transfers and isomerizes the ribose moiety from AdoMet to the
CC 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give
CC epoxyqueuosine (oQ-tRNA). {ECO:0000256|HAMAP-Rule:MF_00113}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-
CC methionine = adenine + epoxyqueuosine(34) in tRNA + 2 H(+) + L-
CC methionine; Xref=Rhea:RHEA:32155, Rhea:RHEA-COMP:10342, Rhea:RHEA-
CC COMP:18582, ChEBI:CHEBI:15378, ChEBI:CHEBI:16708, ChEBI:CHEBI:57844,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:82833, ChEBI:CHEBI:194443;
CC EC=2.4.99.17; Evidence={ECO:0000256|HAMAP-Rule:MF_00113};
CC -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC {ECO:0000256|HAMAP-Rule:MF_00113}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00113}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00113}.
CC -!- SIMILARITY: Belongs to the QueA family. {ECO:0000256|HAMAP-
CC Rule:MF_00113}.
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DR EMBL; CP004021; AKK20527.1; -; Genomic_DNA.
DR RefSeq; WP_047264493.1; NZ_CP004021.1.
DR AlphaFoldDB; A0A0G3I9Q7; -.
DR STRING; 1277257.G293_04560; -.
DR KEGG; lau:G293_04560; -.
DR PATRIC; fig|1277257.4.peg.987; -.
DR OrthoDB; 9805933at2; -.
DR UniPathway; UPA00392; -.
DR Proteomes; UP000035503; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051075; F:S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 2.40.10.240; QueA-like; 1.
DR Gene3D; 3.40.1780.10; QueA-like; 1.
DR HAMAP; MF_00113; QueA; 1.
DR InterPro; IPR003699; QueA.
DR InterPro; IPR042118; QueA_dom1.
DR InterPro; IPR042119; QueA_dom2.
DR InterPro; IPR036100; QueA_sf.
DR NCBIfam; TIGR00113; queA; 1.
DR PANTHER; PTHR30307; S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE; 1.
DR PANTHER; PTHR30307:SF0; S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE; 1.
DR Pfam; PF02547; Queuosine_synth; 1.
DR SUPFAM; SSF111337; QueA-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00113};
KW Isomerase {ECO:0000313|EMBL:AKK20527.1};
KW Queuosine biosynthesis {ECO:0000256|ARBA:ARBA00022785, ECO:0000256|HAMAP-
KW Rule:MF_00113};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_00113};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00113}.
SQ SEQUENCE 360 AA; 40046 MW; AFD30097C1A21444 CRC64;
MMVKEFDFNL PSSRIALRPA SPRDSARLMV VQPNLSGISM ISDHLVSDLP YFLNSNDAIV
FNNTKVITAQ LSGIRLHHIN NKETPISCTL HMRFSSNSWS AYARPGKAIR TGDIILFFSQ
DRQYRLEATV AEKWDTGEIL LTFPLSDMVL ERQISLVGTI PLPPYIARKR PIDARDYVDY
QTTYAKIQGS VAAPTAGLHF TSNLLSRIIS IGVEVHFITL HVGAGTFMPV KVEDTDDHNM
HSEIGVIDIA TAKALNSVKS RGGRIVAVGT TSLRLLETAT TEDGIIMPWS GSTNIFITPG
YCFRAVDVLM SNFHLPKSTL LMLVSAFCGI EETKKIYQHA ISHSYRFYSY GDASLLFPKR
//
