ID A0A0G3WEF3_9CLOT Unreviewed; 457 AA.
AC A0A0G3WEF3;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE SubName: Full=Iron-only hydrogenase maturation protein HydG {ECO:0000313|EMBL:AKL97001.1};
GN Name=hydG {ECO:0000313|EMBL:AKL97001.1};
GN ORFNames=CACET_c35700 {ECO:0000313|EMBL:AKL97001.1};
OS Clostridium aceticum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=84022 {ECO:0000313|EMBL:AKL97001.1, ECO:0000313|Proteomes:UP000035704};
RN [1] {ECO:0000313|EMBL:AKL97001.1, ECO:0000313|Proteomes:UP000035704}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 1496 {ECO:0000313|EMBL:AKL97001.1,
RC ECO:0000313|Proteomes:UP000035704};
RA Poehlein A., Schiel-Bengelsdorf B., Gottschalk G., Duerre P., Daniel R.;
RT "Genome sequence of Clostridium aceticum DSM 1496.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|ARBA:ARBA00034078};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CP009687; AKL97001.1; -; Genomic_DNA.
DR RefSeq; WP_044826306.1; NZ_JYHU01000028.1.
DR AlphaFoldDB; A0A0G3WEF3; -.
DR STRING; 84022.CACET_c35700; -.
DR KEGG; cace:CACET_c35700; -.
DR PATRIC; fig|84022.6.peg.3647; -.
DR OrthoDB; 9801120at2; -.
DR Proteomes; UP000035704; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR010722; BATS_dom.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR024007; FeFe-hyd_mat_HydG.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR012726; ThiH.
DR InterPro; IPR034428; ThiH/NoCL/HydG-like.
DR NCBIfam; TIGR03955; rSAM_HydG; 1.
DR PANTHER; PTHR43583; 2-IMINOACETATE SYNTHASE; 1.
DR PANTHER; PTHR43583:SF2; BIOTIN AND THIAMIN SYNTHESIS ASSOCIATED DOMAIN CONTAINING PROTEIN; 1.
DR Pfam; PF06968; BATS; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00301; 2-iminoacetate_synthase_(ThiH); 1.
DR SFLD; SFLDF00319; Fe_hydrogenase_maturase_(HydG; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00876; BATS; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000035704};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 72..300
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 457 AA; 51965 MW; 754538E13B7E4B82 CRC64;
MDTNKIISEN QINKLLEDYR NTTKEEILRI IEKSQECHGL TLGEVAALLQ TEDEGLMEIL
FDAARFIKNK IYGKRIVVFA PLYTSNECTN NCLYCGFRAA NKKLHRKTLS KEEIIHEAKS
IEKQGHKRIL LVCGEDRRST GIDHIVHAVK TTYEAGDIRR INVNTAPMEV EDFKKLKMAG
IGTYQIFQET YHRETYRKMH PTGAKADYDY RLTAIERALE AGIDDFGIGP LLGLYDYKFD
VLATLRHSQY MDEKYGVGPH TISVPRLKPA LDSGLKEIPY AVSDKDFKKI VAIYRLAVPY
TGIILSTREN ATLRDELLNL GISQISAGSK TNPGGYGKED KEAKQFENSD ERTLDEMLQV
ICEQGHIPSF CTACYRKERT GNAFMELAKD AHIDGFCQVN AILTLKENLL DYASENMVRK
GNTLINRTLN EIKDASIRNL IEERLGQIEM GKRDLYL
//
