ID A0A0G4ED86_VITBC Unreviewed; 625 AA.
AC A0A0G4ED86;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=methylenetetrahydrofolate reductase (NADH) {ECO:0000256|ARBA:ARBA00034529};
DE EC=1.5.1.54 {ECO:0000256|ARBA:ARBA00034529};
GN ORFNames=Vbra_4858 {ECO:0000313|EMBL:CEL93647.1};
OS Vitrella brassicaformis (strain CCMP3155).
OC Eukaryota; Sar; Alveolata; Colpodellida; Vitrellaceae; Vitrella.
OX NCBI_TaxID=1169540 {ECO:0000313|EMBL:CEL93647.1, ECO:0000313|Proteomes:UP000041254};
RN [1] {ECO:0000313|EMBL:CEL93647.1, ECO:0000313|Proteomes:UP000041254}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Zhu J., Qi W., Song R.;
RL Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC pathway. {ECO:0000256|ARBA:ARBA00034478}.
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777}.
CC -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00006743}.
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DR EMBL; CDMY01000182; CEL93647.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G4ED86; -.
DR STRING; 1169540.A0A0G4ED86; -.
DR VEuPathDB; CryptoDB:Vbra_4858; -.
DR InParanoid; A0A0G4ED86; -.
DR OMA; VARTSQW; -.
DR OrthoDB; 1381745at2759; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000041254; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:InterPro.
DR GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IEA:UniProtKB-EC.
DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00537; MTHFR; 1.
DR Gene3D; 3.20.20.220; -; 1.
DR InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR InterPro; IPR004621; Fadh2_euk.
DR InterPro; IPR003171; Mehydrof_redctse-like.
DR InterPro; IPR004620; MTHF_reductase_bac.
DR NCBIfam; TIGR00676; fadh2; 1.
DR NCBIfam; TIGR00677; fadh2_euk; 1.
DR PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF02219; MTHFR; 1.
DR SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Methionine biosynthesis {ECO:0000256|ARBA:ARBA00023167};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000041254}.
FT REGION 599..625
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 604..625
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 625 AA; 70403 MW; 0CA2915E13695A8F CRC64;
MKITDAMCEF EKANEDKAFF SFEFFPPKTE AGVANLYDRF DRMAQLNPLW IDVTWGAGGS
TAQRTLELCT NALKYHGLEP LMHLTCTNMQ KEMLDSALKE CVEHGITNIL ALRGDPPKDQ
ERWTAVEGGL EHASDLVAYI RKNYGDFFCI AVAAYPEGHL ENPDKEKDLQ MLKVKQDAGA
DFAITQLFYD NEEYFAFLEK AKAIGVTIPI LPGIMPIQSY QGFKKMTTFC KTKVPQHIID
DLEPIKDDDQ AVKDYGVKLA VEMCREILDH GVKGLHFYTL NLETSVMRII EGMMLVDTHI
SNRVLPWRPS VHKKRSREDV RPIFWANRPR SYMQRTAAWD EFPNGRWGNR DSPAFDFADH
YISSNVDPNE TSMTHRREMW GEAPSSVADI AKVFCDYLSP ATKVTRLPWC SDIIGDETSI
IRKQLIPLNR NGCLTVNSQP RVNAAISSDP YFGWGPKNGF VFQKAYCEFF CSPETLERIL
MGIEGEKCLS YAAINIDGDI RTNMPEGSVN AVTWGVFPCE EVKQPTVVDL VSFMAWKDEA
FSLWKEEWGA IYPEGSPSRQ LITTIHDSWY LVNIVDNNFV CGDLFGILVG ITGRAKMGSP
KANGSPKANG TINGTDNDVT TDGLV
//
