ID A0A0H2LY14_VARPD Unreviewed; 983 AA.
AC A0A0H2LY14;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE SubName: Full=Na(+)/H(+) antiporter subunit A {ECO:0000313|EMBL:KLN55088.1};
GN Name=mrpA {ECO:0000313|EMBL:KLN55088.1};
GN ORFNames=VPARA_37760 {ECO:0000313|EMBL:KLN55088.1};
OS Variovorax paradoxus.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Variovorax.
OX NCBI_TaxID=34073 {ECO:0000313|EMBL:KLN55088.1, ECO:0000313|Proteomes:UP000035170};
RN [1] {ECO:0000313|EMBL:KLN55088.1, ECO:0000313|Proteomes:UP000035170}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TBEA6 {ECO:0000313|EMBL:KLN55088.1,
RC ECO:0000313|Proteomes:UP000035170};
RA Poehlein A., Schuldes J., Wuebbeler J.H., Hiessl S., Steinbuechel A.,
RA Daniel R.;
RT "Genome sequence of Variovorax paradoxus TBEA6.";
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141, ECO:0000256|RuleBase:RU000320}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141,
CC ECO:0000256|RuleBase:RU000320}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KLN55088.1}.
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DR EMBL; JZWI01000019; KLN55088.1; -; Genomic_DNA.
DR RefSeq; WP_047785619.1; NZ_JZWI01000019.1.
DR AlphaFoldDB; A0A0H2LY14; -.
DR PATRIC; fig|34073.19.peg.3865; -.
DR Proteomes; UP000035170; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:monoatomic ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR007182; MnhB.
DR InterPro; IPR025383; MrpA_C/MbhD.
DR InterPro; IPR046806; MrpA_C/MbhE.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR43373:SF1; ANTIPORTER SUBUNIT MNHA2-RELATED; 1.
DR PANTHER; PTHR43373; NA(+)/H(+) ANTIPORTER SUBUNIT; 1.
DR Pfam; PF13244; MbhD; 1.
DR Pfam; PF20501; MbhE; 1.
DR Pfam; PF04039; MnhB; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR PRINTS; PR01434; NADHDHGNASE5.
PE 4: Predicted;
KW Antiport {ECO:0000256|ARBA:ARBA00022449};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000035170};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU000320};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 76..97
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 109..127
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 162..185
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 205..230
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 242..264
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 270..291
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 367..386
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 406..425
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 460..483
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 503..522
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 575..593
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 605..625
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 632..652
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 658..680
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 701..722
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 760..778
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 815..840
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 852..874
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 886..908
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 928..950
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 65..110
FT /note="NADH-Ubiquinone oxidoreductase (complex I) chain 5
FT N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00662"
FT DOMAIN 126..399
FT /note="NADH:quinone oxidoreductase/Mrp antiporter membrane
FT subunit"
FT /evidence="ECO:0000259|Pfam:PF00361"
FT DOMAIN 618..680
FT /note="MrpA C-terminal/MbhD"
FT /evidence="ECO:0000259|Pfam:PF13244"
FT DOMAIN 699..795
FT /note="MrpA C-terminal/MbhE"
FT /evidence="ECO:0000259|Pfam:PF20501"
FT DOMAIN 822..944
FT /note="Na+/H+ antiporter MnhB subunit-related protein"
FT /evidence="ECO:0000259|Pfam:PF04039"
SQ SEQUENCE 983 AA; 106052 MW; 4A355862AF1AADAD CRC64;
MPLVFLVALP FIASVLAALM PSNARNREST LAGLVALGCA VQTAWFFPQI ARGNVLRQEI
AWLPELGLNL VFRMDGFAWL FCMLVLGIGA LVVLYARYYM SASDPVPRFF SFFLAFMGAM
TGVVLSGNLI QLVLFWELTS LFSFLLIGYW HHRRDARRGA RMALTVTGAG GLCLLAGVVV
LGRIAGSYEL DVVLASGNEI RAHALYPAAL VLILLGAFTK SAQFPFHFWL PRAMAAPTPV
SAYLHSATMV KLGVFLMARL WPVLSGTEQW FWLVGGAGAI TLLLGGFVAM FQRDLKALLA
YSTISHLGLI TLLLGLNSPL AAVAAVFHIM NHATFKASLF MAAGIIDHES GTRDIRKLSG
LLRLMPITGT LAIIASASMA GVPLLNGFLS KEMFFAETVF IQATPWINLS LPVIATVAGI
FSVAYSARFV FDVFFGPPCG PDVPKQPHEP PHWMRVPVEL LVLVCLVVGV APAWSIGALL
AAAAAPVVGG VLPEYSLAVW HGFNLPLAMS FVALAGGMVL YLSQRRRRAQ GKLEHTPLLH
RFDGQAIFEH LLAQLSEAGR RSRRVLGTRR MQSQLLLLIA VAAAGAAASL WTTPVARGAR
ELLPFSPMFA MTWLIGGVCA LAAAWQAKFH RLAALMLASG AGLVCCVTYI WFSAPDLALT
QLVVEAVTTV LILLGLRWLP MRSKDAVQSA RARLRPWGRR GRDLLVAAAA GGGMSALAWL
MMTRPFPQSI SPFFLEHALA EGGGTNVVNV MLVDFRGFDT FGEITVLGVV ALTVYALLRR
FRPARESMAL PAQQRAQADD GSSDLLNPRL AKDTAVGYLM VPAVLVRLLL PLAVLVSVYF
FMRGHNAPGG GFVAGLVMSV ALVLQFIVSG TEWVEEHLRI YPRRWIAAGL LLALVTGSGA
VVFGYPFLTT HTAHLHLPVL GELHVPSALF FDIGVFALVL GATMLILTAL AHQSVRSHRW
ADEQREKEAE AAAAASAMPE GAH
//
