ID A0A0H3C741_CAUVN Unreviewed; 700 AA.
AC A0A0H3C741;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE SubName: Full=Penicillin acylase {ECO:0000313|EMBL:ACL94542.1};
DE EC=3.5.1.11 {ECO:0000313|EMBL:ACL94542.1};
GN OrderedLocusNames=CCNA_01077 {ECO:0000313|EMBL:ACL94542.1};
OS Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=565050 {ECO:0000313|EMBL:ACL94542.1, ECO:0000313|Proteomes:UP000001364};
RN [1] {ECO:0000313|EMBL:ACL94542.1, ECO:0000313|Proteomes:UP000001364}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NA1000 / CB15N {ECO:0000313|Proteomes:UP000001364};
RX PubMed=20472802; DOI=10.1128/JB.00255-10;
RA Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
RA Walunas T.L., Crosson S.;
RT "The genetic basis of laboratory adaptation in Caulobacter crescentus.";
RL J. Bacteriol. 192:3678-3688(2010).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
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DR EMBL; CP001340; ACL94542.1; -; Genomic_DNA.
DR RefSeq; WP_012640121.1; NC_011916.1.
DR RefSeq; YP_002516450.1; NC_011916.1.
DR AlphaFoldDB; A0A0H3C741; -.
DR GeneID; 7329828; -.
DR KEGG; ccs:CCNA_01077; -.
DR PATRIC; fig|565050.3.peg.1060; -.
DR HOGENOM; CLU_021155_0_0_5; -.
DR OrthoDB; 9760084at2; -.
DR PhylomeDB; A0A0H3C741; -.
DR Proteomes; UP000001364; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008953; F:penicillin amidase activity; IEA:UniProtKB-EC.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd01936; Ntn_CA; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF3; ACYL-HOMOSERINE LACTONE ACYLASE PVDQ; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 2.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ACL94542.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000001364};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT REGION 186..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 198
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 181
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 271
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 273
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 274
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 700 AA; 76569 MW; 7F2C606BC85B3F5B CRC64;
MKWILRGIGG LLALVLIAAL ALIAIDRASL PQAPSRQALL DKAKAYDVRI RRDEWGVPHI
LGRTDADAAF GLGYAQSEDD FDTLQDVVLA TRGVLARDKG AGAAPTDYVV SLLEVWPTVD
KHYAELPAEL RAIMEAYADG VSLYVAKHPE KAKPGLLPVT GKDVAAGFIF KQPFFYGLDG
ELRRITAPAT KGPPPKGSNG LATAPSRSVD GATRLLVNSH QPYTGPVAWY EAVVQSGQGW
HVAGGFFPGS PFMLHGHNAT LGWANTVSKP DLVDVYRLTI NPANKNQYRL DGQWKDFDKR
YVTLRVKLLG PIVLPVRKAV LRSAHGPVLE TDHGVFAIRY AGMGEWRQPL QYWRLNRART
VEDWRAAIAT HAIPSLNYVY ADAAGNIGFV HNGQYPNRRA KADWGGVLPG DRSDLIWQGY
LPVENSPQLW NPKSGLVFNS NNTPFEASEA ADNLKPADFP ASMGLQANMT NRAWRALETY
GADRALTDAS FRAHKFDVAF SDRSSVMAMV GEVLAVDPKG DADLAEAQRI LRAWDKRADR
TNRGAALAAL MSQPILFANT NGDPAPAPID SLRAAIKTLK THFGRLDPEW GEVNRIRRGT
VDLPIDGAAD TFRSVWGKPQ KDGTTTADGG DTFVMFVTWD KAGALRSESI HQFGSATLDA
ASPHYADQTP LFVAMKTKPV RFTEDQLQGH IKVDYRPGER
//
