ID A0A0H3J4H2_CLOPA Unreviewed; 142 AA.
AC A0A0H3J4H2;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=Nickel/cobalt efflux system {ECO:0000256|RuleBase:RU362101};
GN Name=nixA2 {ECO:0000313|EMBL:AJA50806.1};
GN ORFNames=CLPA_c07180 {ECO:0000313|EMBL:AJA50806.1}, CP6013_02432
GN {ECO:0000313|EMBL:KRU13184.1};
OS Clostridium pasteurianum DSM 525 = ATCC 6013.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262449 {ECO:0000313|EMBL:AJA50806.1, ECO:0000313|Proteomes:UP000030905};
RN [1] {ECO:0000313|EMBL:AJA50806.1, ECO:0000313|Proteomes:UP000030905}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 525 {ECO:0000313|EMBL:AJA50806.1}, and DSM 525 / ATCC 6013
RC {ECO:0000313|Proteomes:UP000030905};
RX PubMed=25700415;
RA Poehlein A., Grosse-Honebrink A., Zhang Y., Minton N.P., Daniel R.;
RT "Complete Genome Sequence of the Nitrogen-Fixing and Solvent-Producing
RT Clostridium pasteurianum DSM 525.";
RL Genome Announc. 3:e01591-e01514(2015).
RN [2] {ECO:0000313|EMBL:KRU13184.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 6013 {ECO:0000313|EMBL:KRU13184.1};
RA Pyne M.E., Utturkar S.M., Brown S.D., Moo-Young M., Chung D.A., Chou P.C.;
RT "Improved Draft Genome Sequence of Clostridium pasteurianum Strain ATCC
RT 6013 (DSM 525) Using a Hybrid Next-Generation Sequencing Approach.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KRU13184.1, ECO:0000313|Proteomes:UP000028042}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 6013 {ECO:0000313|EMBL:KRU13184.1,
RC ECO:0000313|Proteomes:UP000028042};
RX PubMed=25103768;
RA Pyne M.E., Utturkar S., Brown S.D., Moo-Young M., Chung D.A., Chou C.P.;
RT "Improved Draft Genome Sequence of Clostridium pasteurianum Strain ATCC
RT 6013 (DSM 525) Using a Hybrid Next-Generation Sequencing Approach.";
RL Genome Announc. 2:0-0(0).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU362101};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU362101}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC {ECO:0000256|ARBA:ARBA00010892, ECO:0000256|RuleBase:RU362101}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|RuleBase:RU362101}.
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DR EMBL; CP009268; AJA50806.1; -; Genomic_DNA.
DR EMBL; JPGY02000001; KRU13184.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0H3J4H2; -.
DR KEGG; cpae:CPAST_c07180; -.
DR KEGG; cpat:CLPA_c07180; -.
DR PATRIC; fig|1262449.3.peg.2854; -.
DR eggNOG; COG3376; Bacteria.
DR Proteomes; UP000028042; Unassembled WGS sequence.
DR Proteomes; UP000030905; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR InterPro; IPR004688; Ni/Co_transpt.
DR InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR PANTHER; PTHR31611; HIGH-AFFINITY NICKEL TRANSPORT PROTEIN NIC1; 1.
DR PANTHER; PTHR31611:SF0; HIGH-AFFINITY NICKEL TRANSPORT PROTEIN NIC1; 1.
DR Pfam; PF03824; NicO; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU362101};
KW Nickel {ECO:0000256|ARBA:ARBA00022596};
KW Reference proteome {ECO:0000313|Proteomes:UP000030905};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU362101};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU362101};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU362101}.
FT TRANSMEM 30..55
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 67..90
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 110..134
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
SQ SEQUENCE 142 AA; 15854 MW; D8A7F1DB03FEA2AC CRC64;
MNFLFKKQPK WLPLAISANA ASQAIPFSMI IALPILFAAG MSLMDTADGV FMTTAYNWAF
STPLRKIYYN LSVTGLSIAA ALCIGFIELA QILTPKLGLN SGIWKWIQNI QFGVIGYLLV
GLFIISWAVS YMIWKMFRFE NA
//
