ID   A0A0H5CVG2_9PSEU        Unreviewed;       665 AA.
AC   A0A0H5CVG2;
DT   14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT   14-OCT-2015, sequence version 1.
DT   27-MAR-2024, entry version 31.
DE   SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:CRK61978.1};
OS   Alloactinosynnema sp. L-07.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae.
OX   NCBI_TaxID=1653480 {ECO:0000313|EMBL:CRK61978.1, ECO:0000313|Proteomes:UP000076116};
RN   [1] {ECO:0000313|Proteomes:UP000076116}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L-07 {ECO:0000313|Proteomes:UP000076116};
RA   Ramaraj T.;
RL   Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR   EMBL; LN850107; CRK61978.1; -; Genomic_DNA.
DR   RefSeq; WP_067520988.1; NZ_LN850107.1.
DR   AlphaFoldDB; A0A0H5CVG2; -.
DR   STRING; 1653480.gene:80950886; -.
DR   KEGG; alo:CRK61978; -.
DR   OrthoDB; 5298546at2; -.
DR   Proteomes; UP000076116; Chromosome i.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR   Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR   InterPro; IPR006158; Cobalamin-bd.
DR   InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR   InterPro; IPR034466; Methyltransferase_Class_B.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   NCBIfam; NF033712; B12_rSAM_KedN5; 1.
DR   PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR   PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR   Pfam; PF02310; B12-binding; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR   SFLD; SFLDF00436; pactamycin_C-methyltransferase; 1.
DR   SFLD; SFLDF00317; thioacetal_methlytransferase; 1.
DR   SMART; SM00729; Elp3; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51332; B12_BINDING; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Iron {ECO:0000256|ARBA:ARBA00022485};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW   Reference proteome {ECO:0000313|Proteomes:UP000076116}.
FT   DOMAIN          10..148
FT                   /note="B12-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51332"
FT   DOMAIN          197..426
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
FT   REGION          635..665
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   665 AA;  74435 MW;  A6A16B09CEB025DC CRC64;
     MGRQPLRVWL VQQGIWAMSL ESMPLASGYL KAAALADPVV AADTEIVIHN FDGGATLFGM
     ANALFHDGAP DVLAFSVLGW NYREFTALAE TFKQLNPAGW AVFGGNHVSD QGDRVLTANP
     CVDVVVNGEG EFTFRELLRA RLAGADPREL HDIAGIAFRG GSGEIVSTVD RPRVENLDEI
     PSPFLTGAIP MTDAHGGFPY DVALMETNRG CPYKCSFCYW GGAVGQKVRS FSRERLRAEV
     ELFARHRVHT LVLCDANFGM LPADRDFVAD VVAIRERYGF PRSIEASWAK NKSTVFFDIV
     RTMKEVGLHS SFTLALQTLD DHTLDLMSRR NMKVNDWEEL AAWISFEGMD CYAELIWGAP
     GETVESFMNG YDRLAQHVSR IAVYPMLILP NTTYHQDRKR FGLVTVRGNT DDFEYLISHE
     TMSSDDNRYM HRFIFVSRLV AENPVFRHLW APLRVLGGIT QSAAIRSLMD WFAQDDDPAC
     EPFRTGMVNS FTDSDVLAPV LVHIYGTDEA KDLLRRWWRT VLRPLVPPRH RGVLDATVDY
     DLLTLPAYTA PGRAPSSRYA GEHLPLVDVD GEPHHVLRDV RLACDVPAVL RALRRGEPHE
     PAVRPVTTTL YYKAGADRFL PSTNHEEVVY YMGKPEQVRQ LPDRPIPNAR SRSDVRSGDR
     GSPVG
//