ID A0A0H5D0S4_9PSEU Unreviewed; 642 AA.
AC A0A0H5D0S4;
DT 14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2015, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:CRK58100.1};
OS Alloactinosynnema sp. L-07.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae.
OX NCBI_TaxID=1653480 {ECO:0000313|EMBL:CRK58100.1, ECO:0000313|Proteomes:UP000076116};
RN [1] {ECO:0000313|Proteomes:UP000076116}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=L-07 {ECO:0000313|Proteomes:UP000076116};
RA Ramaraj T.;
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; LN850107; CRK58100.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0H5D0S4; -.
DR STRING; 1653480.gene:80946969; -.
DR KEGG; alo:CRK58100; -.
DR OrthoDB; 9785699at2; -.
DR Proteomes; UP000076116; Chromosome i.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.80.30.30; -; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 1.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR040086; MJ0683-like.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR NCBIfam; NF038135; rSAM_Rv2578c; 1.
DR NCBIfam; NF038136; rSAM_Rv_intein; 1.
DR PANTHER; PTHR43432; SLR0285 PROTEIN; 1.
DR PANTHER; PTHR43432:SF3; SLR0285 PROTEIN; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Reference proteome {ECO:0000313|Proteomes:UP000076116}.
FT DOMAIN 199..285
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 351..372
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT DOMAIN 358..600
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 642 AA; 69476 MW; B20F975350602B93 CRC64;
MCSTGVVRWD GQLAAGGEQA LPGLPGLVRS VRTPEFSGMV FHEVLAKSVL NKVAGGSSLP
FGWTVNPYRG CSHACSYCLH GDTPILMADG TTKPISALRV GDRVYGTERR GSYRHYVTTE
VRDHWSTVKP AYRVTLEDGT VLIASGDHRF LTERGWKHVT GAVAGPDRRP HLTVGNKLMG
TGKFAPPPKD TAEYRRGYLC GVARGDGGAA PAEQVQARVS AYLLDDLGLS LPVEWPDTPT
DEWRKGLLAG VFDTDGGYAH RIVRFANLSE RTATAVLEAL ARFGFQSAVE ERRVPGGRCC
VRVDGGLAEH LRLFHTVGPA VTAKRTITDA AIKSAARLGV LEIRPLGLSL PLFDITTGTG
DFIANGVVSH NCFARNTHTY LDLDAGHDFD SQIVVKVNAP EVLARQLRAK SWTREHVAMG
TNTDPYQRAE GRYQLMPGII RALADSGTPF SILTKGTVLF RDLPLLTSVS ADVPVGLGVS
IALLDRDVQR RLEPGTASPQ ARLELVRRAR EAGLPCAVMV APVLPGLTDS REQLDALLAQ
IARAGATGVT VLPLHLRPGA REWFAAWLAR DHPALVPLYR RIYGTGTNAD IRYRRALGTR
VGPLLRRHGF AGPDIPEPRW PRGSLPHGAP DVPVAQDQLT LL
//
