ID A0A0J6W288_9MYCO Unreviewed; 321 AA.
AC A0A0J6W288;
DT 14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2015, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE SubName: Full=Hydrogenase {ECO:0000313|EMBL:KKF00958.1, ECO:0000313|EMBL:KMO75807.1};
DE EC=1.12.2.1 {ECO:0000313|EMBL:KMO75807.1};
GN Name=hydA {ECO:0000313|EMBL:KMO75807.1};
GN ORFNames=EUA04_06085 {ECO:0000313|EMBL:TDL09547.1}, MOBUDSM44075_02896
GN {ECO:0000313|EMBL:KMO75807.1}, WN67_16265
GN {ECO:0000313|EMBL:KKF00958.1};
OS Mycolicibacterium obuense.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=1807 {ECO:0000313|EMBL:KMO75807.1, ECO:0000313|Proteomes:UP000036313};
RN [1] {ECO:0000313|EMBL:KMO75807.1, ECO:0000313|Proteomes:UP000036313}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44075 {ECO:0000313|EMBL:KMO75807.1,
RC ECO:0000313|Proteomes:UP000036313};
RX PubMed=26079817; DOI=10.1093/gbe/evv111;
RA Das S., Pettersson B.M., Behra P.R., Ramesh M., Dasgupta S.,
RA Bhattacharya A., Kirsebom L.A.;
RT "Characterization of Three Mycobacterium spp. with Potential Use in
RT Bioremediation by Genome Sequencing and Comparative Genomics.";
RL Genome Biol. Evol. 7:1871-1886(2015).
RN [2] {ECO:0000313|EMBL:KKF00958.1, ECO:0000313|Proteomes:UP000034150}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UC1 {ECO:0000313|EMBL:KKF00958.1,
RC ECO:0000313|Proteomes:UP000034150};
RA Greninger A.L., Cunningham G., Chiu C.Y., Miller S.;
RT "Genome sequence of Mycobacterium obuense UC1.";
RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:TDL09547.1, ECO:0000313|Proteomes:UP000294952}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=22DIII {ECO:0000313|EMBL:TDL09547.1,
RC ECO:0000313|Proteomes:UP000294952};
RA Tiago I., Alarico S., Pereira S.G., Coelho C., Maranha A., Empadinhas N.;
RT "High-quality-draft genome sequences of five non-tuberculosis
RT mycobacteriaceae isolated from a nosocomial environment.";
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC {ECO:0000256|ARBA:ARBA00011771}.
CC -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit
CC family. {ECO:0000256|ARBA:ARBA00006605}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KMO75807.1}.
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DR EMBL; LAUZ02000053; KKF00958.1; -; Genomic_DNA.
DR EMBL; JYNU01000015; KMO75807.1; -; Genomic_DNA.
DR EMBL; SDLP01000002; TDL09547.1; -; Genomic_DNA.
DR RefSeq; WP_046364081.1; NZ_SDLP01000002.1.
DR STRING; 1807.MOBUDSM44075_02896; -.
DR PATRIC; fig|1807.13.peg.4116; -.
DR OrthoDB; 9766729at2; -.
DR Proteomes; UP000034150; Unassembled WGS sequence.
DR Proteomes; UP000036313; Unassembled WGS sequence.
DR Proteomes; UP000294952; Unassembled WGS sequence.
DR GO; GO:0009375; C:ferredoxin hydrogenase complex; IEA:InterPro.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0047806; F:cytochrome-c3 hydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 4.10.480.10; Cytochrome-c3 hydrogenase, C-terminal domain; 1.
DR Gene3D; 3.40.50.700; NADH:ubiquinone oxidoreductase-like, 20kDa subunit; 1.
DR InterPro; IPR027394; Cytochrome-c3_hydrogenase_C.
DR InterPro; IPR006137; NADH_UbQ_OxRdtase-like_20kDa.
DR InterPro; IPR037148; NiFe-Hase_small_C_sf.
DR InterPro; IPR037024; NiFe_Hase_small_N_sf.
DR InterPro; IPR001821; NiFe_hydrogenase_ssu.
DR PANTHER; PTHR30013:SF5; HYDROGENASE SMALL SUBUNIT; 1.
DR PANTHER; PTHR30013; NIFE / NIFESE HYDROGENASE SMALL SUBUNIT FAMILY MEMBER; 1.
DR Pfam; PF14720; NiFe_hyd_SSU_C; 1.
DR Pfam; PF01058; Oxidored_q6; 1.
DR PIRSF; PIRSF000310; NiFe_hyd_ssu; 1.
DR SUPFAM; SSF56770; HydA/Nqo6-like; 1.
PE 3: Inferred from homology;
KW 3Fe-4S {ECO:0000256|PIRSR:PIRSR000310-1};
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|PIRSR:PIRSR000310-1};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR000310-1};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRSR:PIRSR000310-
KW 1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR000310-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:KMO75807.1}; Signal {ECO:0000256|ARBA:ARBA00022729}.
FT DOMAIN 12..173
FT /note="NADH:ubiquinone oxidoreductase-like 20kDa subunit"
FT /evidence="ECO:0000259|Pfam:PF01058"
FT DOMAIN 194..268
FT /note="Cytochrome-c3 hydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14720"
FT BINDING 12
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 111
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 159
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 201
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 224
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 231
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 240
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 258
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
FT BINDING 261
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000256|PIRSR:PIRSR000310-1"
SQ SEQUENCE 321 AA; 35051 MW; 456C29C96C725C52 CRC64;
MASVLWFQGG ACSGNTMSFL NAEEPNVVDL IVDFGLDLLW HPSLGLELGQ NAQKLFSDCA
TGQRPLDIFV FEGTVMEAYG GRADMFADRP MKDWVTDLAG AAQIVVAIGD CACWGGIPAM
EPNPSGSTGL QFHKREKGGF LGPDFRSKMG LPVINVPGCP AHPDWITQIL VALATGRAGD
ITLDDLHRPE TFFKTFTQTG CTRVQFFEYK QSTMSFGEGT RTGCLFYEFG CRGPMTHSPC
NRILWNRQSS KTRAGMPCLG CTEPEFPHFD LAPGTLFKTQ KVSGMIPKEV PEGTDHLTYM
GLAAAARIAA PQWSKEDMFV V
//
