ID A0A0J6WXM8_9FIRM Unreviewed; 875 AA.
AC A0A0J6WXM8;
DT 14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2015, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=AB840_03075 {ECO:0000313|EMBL:KMO87384.1};
OS Megasphaera cerevisiae DSM 20462.
OC Bacteria; Bacillota; Negativicutes; Veillonellales; Veillonellaceae;
OC Megasphaera.
OX NCBI_TaxID=1122219 {ECO:0000313|EMBL:KMO87384.1, ECO:0000313|Proteomes:UP000036503};
RN [1] {ECO:0000313|EMBL:KMO87384.1, ECO:0000313|Proteomes:UP000036503}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20462 {ECO:0000313|EMBL:KMO87384.1,
RC ECO:0000313|Proteomes:UP000036503};
RA Kutumbaka K., Pasmowitz J., Mategko J., Reyes D., Friedrich A., Han S.,
RA Martens-Habbena W., Neal-McKinney J., Janagama H.K., Nadala C.,
RA Samadpour M.;
RT "Draft genome sequence of beer spoilage bacterium Megasphaera cerevisiae
RT type strain 20462.";
RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KMO87384.1}.
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DR EMBL; LEKT01000006; KMO87384.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0J6WXM8; -.
DR STRING; 39029.BSR42_00465; -.
DR PATRIC; fig|1122219.3.peg.2493; -.
DR InParanoid; A0A0J6WXM8; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000036503; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR CDD; cd06127; DEDDh; 1.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.30.160.800; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR00573; dnaq; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00929; RNase_T; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Exonuclease {ECO:0000313|EMBL:KMO87384.1};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000313|EMBL:KMO87384.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000036503}.
FT DOMAIN 5..317
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 318..799
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 875 AA; 100544 MW; 74909463753D33D3 CRC64;
MDVLEGLNEA QREAVLDLDH NILLSAPAGT GKTRILACRT AQILAAKRAE GAQILCLTFT
NRACKELQRR ILTIVKEKGL DVIVKTIHSF CYSILKEETK HTAAIFSDFL VYDDPDCKTI
ISTLTASAPV PLYESKKLQK LQNFIEQIKK FRVLNGPCTR NRQEDYASAV HTLLCDSCQV
QKLCRNYDGS YDTAMEQWLA TNGLTLLCQY DAALSANHAV DFADLITGVC ELFHDPEICR
RWRERFAYIS VDEMQDTSEI EYALISQLFP GRNILLCGDY FQTIYEWRGS YPDLILRRFT
ESYKPRRITF TVNYRSTQLL LHATAACLVN LFGPPAASVR SVAAASDPGL PIIVHEAAQF
MDEARWIFQE ILKLPPTDWP HICIMTRTNP QNKQIWNGVR SHNETLPPDR RLPFTMIDQF
QLFKRQESKD VIAFLRLLIH KNDTISLTRI LKRFSSRIGS RTIMAIESPS YHQLGIRLTD
FIDPSAHTFH DPFGLLLHAL EQERIVVFDV ESTGTDIIHD EIIQIAAIRL NTQGRTAEKF
TAYVRPSRNV GTSFYVHHIS DEQLARDGKD PRIILPALLD FIKDGVIVGH NVTYDLSILH
SELRRLHIPA PAGLPYYDTL DLFRRFYPNL PNHTLSFLST YFALDDKPSH DALDDILATA
ALLRYAIRQH IRPQTGQRCA AMAIYLPLFR PVSQEMQQLY RQSYVKRPYE MISAVMNDCR
IKEYYEKHAD RTDGENHIDR IENIRRLYRV AQETDSPDKD PRDCLNEFIQ MTALSNSELD
SLLEKKPQIP IITIHQAKGL EFTYVFLAGL QEGTFPLSRS QSGRELEEEK RLFYVAVTRA
KKRLYLSWHR QDGSHRRQPS RFIAAIPDSY KEIQP
//
