ID A0A0J7LM06_9FLAO Unreviewed; 490 AA.
AC A0A0J7LM06;
DT 14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2015, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:KMQ70105.1};
GN ORFNames=ACM44_14075 {ECO:0000313|EMBL:KMQ70105.1};
OS Chryseobacterium koreense CCUG 49689.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Weeksellaceae;
OC Chryseobacterium group; Chryseobacterium.
OX NCBI_TaxID=1304281 {ECO:0000313|EMBL:KMQ70105.1, ECO:0000313|Proteomes:UP000035900};
RN [1] {ECO:0000313|EMBL:KMQ70105.1, ECO:0000313|Proteomes:UP000035900}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 49689 {ECO:0000313|EMBL:KMQ70105.1,
RC ECO:0000313|Proteomes:UP000035900};
RX PubMed=15545478; DOI=10.1099/ijs.0.02998-0;
RA Kim M.K., Im W.T., Shin Y.K., Lim J.H., Kim S.H., Lee B.C., Park M.Y.,
RA Lee K.Y., Lee S.T.;
RT "Kaistella koreensis gen. nov., sp. nov., a novel member of the
RT Chryseobacterium-Bergeyella-Riemerella branch.";
RL Int. J. Syst. Evol. Microbiol. 54:2319-2324(2004).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KMQ70105.1}.
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DR EMBL; LFNG01000034; KMQ70105.1; -; Genomic_DNA.
DR RefSeq; WP_048500695.1; NZ_LFNG01000034.1.
DR AlphaFoldDB; A0A0J7LM06; -.
DR STRING; 1304281.ACM44_14075; -.
DR PATRIC; fig|1304281.5.peg.3054; -.
DR OrthoDB; 9806009at2; -.
DR Proteomes; UP000035900; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR Gene3D; 2.40.30.140; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000035900}.
FT DOMAIN 3..400
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 232
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 262
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 103
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 201
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 236
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 490 AA; 56306 MW; EEFCC979E3E690D6 CRC64;
MNATLLQSFH WYSEGNGTLY RHIKDSSDWL KELGITAVWF PPAYKGAGGG YSVGYDPYDL
FDLGEFDQKG TIPTKYGTKE QYLEVVQTLK EKGIGIVVDI VMNHKAGGDE KEKFQVKKAD
EHNRNNMVSD VFEIESYTKF TFPGRGKQYS DFEWNFTCFS AVDFAEGQEK GIYKIINDYG
DDWEELITDE KGNYTYLMHN DIEHRNPFVR EELNYWGKWY HEQVGFNGVR LDAVKHQSPE
FYKEWLQLLR SNTGENIFAV GEYWAPGQLH LLQKYIEETA GSMSLFDSSL QQNFHLASKA
GAAYDLRKIF DQTLTLADPE HSVTVVANHD TQPLQQLESV VEPWFKPIAY ALILLRENGY
PCIFYPDLYG ASYRDQGNDG NDYEIVLEKV DGIEQLLKAR KENAYGMQRD YFEDANCLGW
IREGDGIHKG CAVVISNKDQ YEKPMEMGRP YSGQIFHDLL GRFEEKVTID ENGWGNFPVP
AGNVSVWVPE
//
