ID A0A0J8D6R0_CLOCY Unreviewed; 855 AA.
AC A0A0J8D6R0;
DT 14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2015, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=pcrA {ECO:0000313|EMBL:KMT21770.1};
GN ORFNames=CLCY_3c00370 {ECO:0000313|EMBL:KMT21770.1};
OS Clostridium cylindrosporum DSM 605.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1121307 {ECO:0000313|EMBL:KMT21770.1, ECO:0000313|Proteomes:UP000036756};
RN [1] {ECO:0000313|EMBL:KMT21770.1, ECO:0000313|Proteomes:UP000036756}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 605 {ECO:0000313|EMBL:KMT21770.1,
RC ECO:0000313|Proteomes:UP000036756};
RA Poehlein A., Schiel-Bengelsdorf B., Bengelsdorf F., Daniel R., Duerre P.;
RT "Draft genome sequence of the purine-degrading Clostridium cylindrosporum
RT HC-1 (DSM 605).";
RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KMT21770.1}.
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DR EMBL; LFVU01000026; KMT21770.1; -; Genomic_DNA.
DR RefSeq; WP_048570431.1; NZ_LFVU01000026.1.
DR AlphaFoldDB; A0A0J8D6R0; -.
DR STRING; 1121307.CLCY_3c00370; -.
DR PATRIC; fig|1121307.3.peg.1391; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000036756; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProt.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR CDD; cd06127; DEDDh; 1.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.30.160.800; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR00573; dnaq; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00929; RNase_T; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000036756}.
FT DOMAIN 1..299
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 305..778
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 22..29
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 855 AA; 98661 MW; 17B7FE8376DC646D CRC64;
MDLNESQQRV VSEIDRNILL LASAGTGKTD TMSRRIANII KSGRANSDEI LCITFTNKAA
KEMENRIKAI IQEEGNKITI RTFHSFCFDI IKREAKRSTD IFTDFVVFDE DDCRDIIKGF
RPHGFSEDSI QRFINIVKEE SVKKGISYNA SIENIFNSRD DDINYICTEN KKVNFDLKRY
LEANGENLIH IYNRELSNNH GLDFNDLIIK AKEILSNENV VNYLRQKYKY INVDEVQDTS
TLEYSIIESI FYDNNILICG DIFQTIYGWR GSEPRKIISN FTEKYSPIEV VFNKNYRATK
NLTEASLGYL LNAFNSEAMS VYSDGLESEA KDLGDKIVFK ENDSLYEEAS FIYSQIKSLQ
IKGEDISKVC VLTRDNGYNV NLSKELSYIQ KGGQGFEFVL VDQFKFFRRM EVKDIIAFLK
LIGNKNDSLS LKRILKRLPT GIGDKTLEEI ESSSYRLAGI SLSDFIDDKT YKGEYFSLLI
EEFKKDNVII FDVESTGVDV TEDEIIQIAA IKINSKGEKV ESFEKFISPK RLVKDSQFVH
GFSDDFLKKN GEDKKKVLNE FLEFSKDAVI VGHNVQFDIN ILTSEVSREK LGEIKFKGFY
DTLDIYRRFH SNVENHKLET LSNVFKTKNK PSHNAMDDIL ATGELLARAI SEDIVPTSFE
RISYIAKHIK SFSDISNKLN NLFKFANENR PCDIITFIVE NFNIKSLYKG EDGYNKMERI
RDFYVLLKDL DDSLKSNRDS LLEVIKITSL SNGELENIII KRSGKIRIPI ITVHQSKGLE
YENVFIAGLQ DGKFPSYRAI KSNDIDEEKR TFYVAITRAK KRLYLSCNTN DGYYKRSLRS
RFIDLIPQKY VKYDN
//
