ID   A0A0J8VK67_9ENTR        Unreviewed;       617 AA.
AC   A0A0J8VK67;
DT   14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT   14-OCT-2015, sequence version 1.
DT   27-MAR-2024, entry version 30.
DE   SubName: Full=Protease 4 {ECO:0000313|EMBL:KMV33853.1};
GN   ORFNames=ACH50_14165 {ECO:0000313|EMBL:KMV33853.1};
OS   Franconibacter pulveris.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Franconibacter.
OX   NCBI_TaxID=435910 {ECO:0000313|EMBL:KMV33853.1, ECO:0000313|Proteomes:UP000037315};
RN   [1] {ECO:0000313|EMBL:KMV33853.1, ECO:0000313|Proteomes:UP000037315}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DJ34 {ECO:0000313|EMBL:KMV33853.1,
RC   ECO:0000313|Proteomes:UP000037315};
RA   Pal S., Banerjee T.D., Roy A., Sar P., Kazy S.K.;
RT   "Genome sequencing of Cronobacter sp. strain DJ34 isolated from petroleum
RT   contaminated sludge of Duliajan Oil Fields, Assam, India.";
RL   Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase S49 family.
CC       {ECO:0000256|ARBA:ARBA00008683}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KMV33853.1}.
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DR   EMBL; LFEJ01000018; KMV33853.1; -; Genomic_DNA.
DR   RefSeq; WP_024558043.1; NZ_LFEJ01000018.1.
DR   AlphaFoldDB; A0A0J8VK67; -.
DR   STRING; 1121863.GCA_000621185_02969; -.
DR   PATRIC; fig|1656095.3.peg.724; -.
DR   OrthoDB; 9764363at2; -.
DR   Proteomes; UP000037315; Unassembled WGS sequence.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006465; P:signal peptide processing; IEA:InterPro.
DR   CDD; cd07019; S49_SppA_1; 1.
DR   CDD; cd07018; S49_SppA_67K_type; 1.
DR   Gene3D; 6.20.330.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR004634; Pept_S49_pIV.
DR   InterPro; IPR004635; Pept_S49_SppA.
DR   InterPro; IPR002142; Peptidase_S49.
DR   InterPro; IPR033854; S49_SppA_1.
DR   InterPro; IPR047217; S49_SppA_67K_type_N.
DR   NCBIfam; TIGR00705; SppA_67K; 1.
DR   NCBIfam; TIGR00706; SppA_dom; 1.
DR   PANTHER; PTHR33209; PROTEASE 4; 1.
DR   PANTHER; PTHR33209:SF1; SERINE PROTEASE SPPA, CHLOROPLASTIC; 1.
DR   Pfam; PF01343; Peptidase_S49; 2.
DR   PIRSF; PIRSF001217; Protease_4_SppA; 1.
DR   SUPFAM; SSF52096; ClpP/crotonase; 2.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000313|EMBL:KMV33853.1};
KW   Serine protease {ECO:0000256|ARBA:ARBA00022825};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        21..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          140..293
FT                   /note="Peptidase S49"
FT                   /evidence="ECO:0000259|Pfam:PF01343"
FT   DOMAIN          391..542
FT                   /note="Peptidase S49"
FT                   /evidence="ECO:0000259|Pfam:PF01343"
FT   ACT_SITE        208
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
FT   ACT_SITE        408
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
SQ   SEQUENCE   617 AA;  66548 MW;  3B686D9663C6A322 CRC64;
     MRTLWRIIAG FFKWTWRLLN FIRELVMNLF FILLILAGVG IWMQLGGGES SQPGRGALLL
     DITGVVVDKP SVNNKLGALG RQLFGANSDR LQENSLFDIV AAIRQAKNDR NITGIVLDLK
     DFAGADQPSM QYIGKALREF RDSGKPVLAV GDSYTQGQYY LASFANKIWL SPQGEVDLHG
     FATNGLYYKS LLDKLKVSTH VFRVGTYKSA VEPFIRDDMS PQAREADSRW IGELWQNYLN
     TLAANRQIPA SQVFPGAQGL LAGLQKAGGD TAKYALDNKL VDALASSAEV EKALVKQFGW
     SKKDKDFSAV SLYDYPVNKP TQQGASVAVI FANGAIMDGE ETQGNVGGDT TASQIRDARL
     DPQVKAIVLR VNSPGGSVTA SEVIRAELAA AKAAGKPVVV SMGGMAASGG YWISTPANYI
     VANPSTLTGS IGIFGVINTM ENSLDAIGVH TDGVATSPLA DVSVTKALPP EVQQMMQLTI
     ENGYQRFINL VAQSRSKTPQ QIDEIAQGHV WTGQDAKNNG LVDSLGDFDD AVAKAAELAK
     LKSWHVDYYQ DEPTFMDMVL SGVSGSVQAM LPQALQAYLP APLSEAAKTA KAESDKLAAF
     NDPQNRYAFC LTCGNVR
//