ID A0A0J8VMK0_9ENTR Unreviewed; 469 AA.
AC A0A0J8VMK0;
DT 14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2015, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=Sucrose-6-phosphate hydrolase {ECO:0000256|RuleBase:RU362110};
DE EC=3.2.1.26 {ECO:0000256|RuleBase:RU362110};
DE AltName: Full=Invertase {ECO:0000256|RuleBase:RU365015};
GN ORFNames=ACH50_11260 {ECO:0000313|EMBL:KMV34718.1};
OS Franconibacter pulveris.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Franconibacter.
OX NCBI_TaxID=435910 {ECO:0000313|EMBL:KMV34718.1, ECO:0000313|Proteomes:UP000037315};
RN [1] {ECO:0000313|EMBL:KMV34718.1, ECO:0000313|Proteomes:UP000037315}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DJ34 {ECO:0000313|EMBL:KMV34718.1,
RC ECO:0000313|Proteomes:UP000037315};
RA Pal S., Banerjee T.D., Roy A., Sar P., Kazy S.K.;
RT "Genome sequencing of Cronobacter sp. strain DJ34 isolated from petroleum
RT contaminated sludge of Duliajan Oil Fields, Assam, India.";
RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Enables the bacterium to metabolize sucrose as a sole carbon
CC source. {ECO:0000256|RuleBase:RU365015}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000256|RuleBase:RU362110};
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC {ECO:0000256|RuleBase:RU365015}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|RuleBase:RU365015}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KMV34718.1}.
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DR EMBL; LFEJ01000014; KMV34718.1; -; Genomic_DNA.
DR RefSeq; WP_048887997.1; NZ_LFEJ01000014.1.
DR AlphaFoldDB; A0A0J8VMK0; -.
DR STRING; 1121863.GCA_000621185_02177; -.
DR PATRIC; fig|1656095.3.peg.1284; -.
DR OrthoDB; 9801455at2; -.
DR UniPathway; UPA00238; -.
DR Proteomes; UP000037315; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005985; P:sucrose metabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd18623; GH32_ScrB-like; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR006232; Suc6P_hydrolase.
DR NCBIfam; TIGR01322; scrB_fam; 1.
DR PANTHER; PTHR43101; BETA-FRUCTOSIDASE; 1.
DR PANTHER; PTHR43101:SF1; BETA-FRUCTOSIDASE; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU365015};
KW Cytoplasm {ECO:0000256|RuleBase:RU365015};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110}.
FT DOMAIN 31..336
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 401..447
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
SQ SEQUENCE 469 AA; 53105 MW; FD90D758AA0AD881 CRC64;
MASTTRLPAI LQAVMKGQPQ ALRDDHYPAW HLAPVTGLLN DPNGFIHFQG RYHLFYQWNP
LGCEHKYKCW GHWSSADLVQ WRHEPIALMP DEEYDRNGCY SGSAVDNQGK LTLCYTGNVK
FDDGGRTAWQ CLAVQNAEGG FDKRGPVVPL PGGYTGHVRD PKVWRHEHHW YMVLGAQDLE
LQGKVLLLRS ENLWNWQSLG EIAGSGLNGI GDAGYMWECP DLFALGGKTF LICCPQGVER
EEKRFLNAHP SAYLSGEFDY ARASFQHGPL NEIDAGFEFY APQTTLTADG RRLLVGWMGV
PDGEEMHQPT TAYGWIHQMT CVRELSEQNG KLYQQPVREL QALRGEEERY QGNAAAAPAL
NAQRLELELE SAGDVTLNFA DTLLVEWRRD ALRLARRSLQ TGEWLYRYWQ GDAHKLQILC
DSSSVEIFIN EGEGVMSSRY FPTQPAQLTF SGSAELRARY WSLRPCMVE
//
