ID A0A0J8YWB7_9GAMM Unreviewed; 1160 AA.
AC A0A0J8YWB7;
DT 14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2015, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=dnaE {ECO:0000313|EMBL:KMV73931.1};
GN ORFNames=AI29_08795 {ECO:0000313|EMBL:KMV73931.1};
OS bacteria symbiont BFo2 of Frankliniella occidentalis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales.
OX NCBI_TaxID=1628856 {ECO:0000313|EMBL:KMV73931.1, ECO:0000313|Proteomes:UP000036794};
RN [1] {ECO:0000313|EMBL:KMV73931.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BFo2 {ECO:0000313|EMBL:KMV73931.1};
RA Facey P.D., Hitchings M.D., Hegarty M.J., Pachebat J.A., Morgan L.V.,
RA Hoeppner J.E., Whitten M.A., Dyson P.J., Del Sol R.;
RT "The draft genomes, genome evolution and classification of BFo1 and BFo2
RT two insect symbionts isolated from Western Flower Thrips (Frankliniella
RT occidentalis [Pergande]).";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KMV73931.1}.
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DR EMBL; JMSP01000071; KMV73931.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0J8YWB7; -.
DR STRING; 1628856.WB67_02705; -.
DR PATRIC; fig|1628856.3.peg.1760; -.
DR Proteomes; UP000036794; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR048472; DNA_pol_IIIA_C.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF20914; DNA_pol_IIIA_C; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000313|EMBL:KMV73931.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000036794};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:KMV73931.1}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1160 AA; 130083 MW; C6422201A67B808C CRC64;
MANPRFIHLH VHSDYSMIDG IAKTGALVKK AASLGMPALG ITDHMNLCGL VKFYGAALGA
GVKPIIGADL KVINPLLGDE PMQLTVLAAN NEGYQNLTLL ISKAYQRGYG HQGPIIDQQW
LIEHAPGLIL LSGGFKGDVG YSLLRGNTAL AEQCLAFYQE YFPDNYYLEL TRTGRNDEEN
YLHQALQLAV DHQIPVVASN DVCFLSHQDF DAHEIRVAIH DGYTLDDPKR PKLYTEQQYL
RTEEEMCELF ADIPEALANS VEIARRCNVT IRLGEYFLPQ FPTGEMTTED FLVASSKAGL
EERLVFLFPD DEVRAAKRPA YDERLEIELT VINQMGFPGY FLIVMEFIQW SKDNGVPVGP
GRGSGAGSLV AYALKITDLD PLEFDLLFER FLNPERVSMP DFDVDFCMEK RDLVIDHVAE
MYGREAVSQI ITFGTMAAKA VIRDVGRVLG HPYGFVDRIS KLIPPDPGMT LEKAFIAEPQ
LPEIYEADEE VKALIDMARK LEGVTRNAGK HAGGVVIAPT KITDFAPLYC DELGQHPVTQ
FDKNDVEYAG LVKFDFLGLR TLTIINWALQ MINARREKQG EPPINIDAIP LDDKKSFDML
QRAETTAVFQ LESRGMKDLI KRLKPDCFED MIALVALFRP GPLQSGMVDN FIDRKHGREA
ISYPDIQWQH ETLQPVLEPT YGIILYQEQV MQIAQVLSGY TLGGADMLRR AMGKKKPEEM
AKQRSVFKEG AERMGVDGEL SMKIFDLVEK FAGYGFNKSH SAAYALVSYQ TLWLKAHYPA
EFMAAVMTAD MDNTEKVVGL VDECWRMGLK VLPPDINAGL YPFHVNNDGE IVYGIGAIKG
VGEGPIEAIL EARNQGGYFK ELFDLCARTD IKKINRRVME KLIMSGAFDR LGPHRAALMS
ALNDALKAAD QHSKAEATGQ GDMFGVLAEE PEQIEQSYAS INRWPEQVQL DGERETLGLY
LTGHPINQYL KEIERYVGGM RLKDMHPTER GKITIAAGLV LAARVMVTKR GNRIGICTLD
DRSGRLEVML YTDALDKYQQ LLEKDKILIV SGQVSFDDFS GGLKMIAREV MDIDEAREKY
ARGLALSLMD RQIDDQLLNR LRQVLEPHRS GTIPVNLYYQ RDDARAKLRF GATWRVTPSD
RLLNDLRLVI GTEQVELEFD
//
