ID A0A0K0Y489_9RHOB Unreviewed; 1040 AA.
AC A0A0K0Y489;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=OSB_12430 {ECO:0000313|EMBL:AKS45798.1}, SAMN05444287_0897
GN {ECO:0000313|EMBL:SIO00773.1};
OS Octadecabacter temperatus.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Octadecabacter.
OX NCBI_TaxID=1458307 {ECO:0000313|EMBL:AKS45798.1, ECO:0000313|Proteomes:UP000067444};
RN [1] {ECO:0000313|EMBL:AKS45798.1, ECO:0000313|Proteomes:UP000067444}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB1 {ECO:0000313|EMBL:AKS45798.1,
RC ECO:0000313|Proteomes:UP000067444};
RX PubMed=26358607;
RA Voget S., Billerbeck S., Simon M., Daniel R.;
RT "Closed Genome Sequence of Octadecabacter temperatus SB1, the First
RT Mesophilic Species of the Genus Octadecabacter.";
RL Genome Announc. 3:e01051-15(2015).
RN [2] {ECO:0000313|EMBL:SIO00773.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 26878 {ECO:0000313|EMBL:SIO00773.1};
RA Varghese N., Submissions S.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000184809}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 26878 {ECO:0000313|Proteomes:UP000184809};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
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DR EMBL; CP012160; AKS45798.1; -; Genomic_DNA.
DR EMBL; FSRP01000001; SIO00773.1; -; Genomic_DNA.
DR RefSeq; WP_049834153.1; NZ_FSRP01000001.1.
DR AlphaFoldDB; A0A0K0Y489; -.
DR REBASE; 116706; OteSB1ORF12430P.
DR KEGG; otm:OSB_12430; -.
DR PATRIC; fig|1458307.3.peg.1257; -.
DR OrthoDB; 9803459at2; -.
DR Proteomes; UP000067444; Chromosome.
DR Proteomes; UP000184809; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR011639; MethylTrfase_TaqI-like_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR041635; Type_ISP_LLaBIII_C.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR Pfam; PF07669; Eco57I; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF18135; Type_ISP_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:AKS45798.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000067444};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 284..388
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT DOMAIN 446..537
FT /note="Type II methyltransferase M.TaqI-like"
FT /evidence="ECO:0000259|Pfam:PF07669"
FT DOMAIN 661..1008
FT /note="Type ISP restriction-modification enzyme LLaBIII C-
FT terminal specificity"
FT /evidence="ECO:0000259|Pfam:PF18135"
SQ SEQUENCE 1040 AA; 119188 MW; 05DD0F39C9EECB56 CRC64;
MSQRLINSYL ADIDKIRMVS GSLTEGVISE AFKDLLKAWA KSDGLTFINQ YPMQSSLKKP
IRPDGAILHD VRVPLGYWEA KDTDDDLDAE IKAKLLTGYP QDNIIFEDSR VAVLWQDRNE
ITRCDMTDTA GLEQLLNLFF GYQRPEIAQF RLAVEQFKAD LPAVLTALRE KIDEAYKINV
TFKTAAKKFL AHACETINPT LGRDDVREML IQHILTEEIF ARVFDEADFH RDNNVAKLLY
ELEGAFFTGA VKKSTLKALQ PYYTAINANA AQITSHTEKQ TFLKVIYENF YKVYNPDAAD
RLGVVYTPNE IVKFMIEGAD WLTDKHFGKR LIDEGVEILD PATGTGTYIC ELIEYFRGQP
DKLRHKYTEE LHANEVAILP YYVANLNIEA TYQAVSGQYA EYPNLCFVDT LDNIGGLGIK
SGHQFDMFAS VSDENVARVK RQNKRKISVV IGNPPYNANQ QNENDNNKNR TYPHIDDLIK
RSFVKLSTAQ KTKVYDMYAR FFRWASDRLH DDGVIAFVTN RSFIDSRTFD GFRRYVAQEF
DEIYLVDLGG DVRANPKLSG TKNNVFGIQT GVAISFFVRR QLGKKKKRDC KIHYVRRPEL
ETSDDKLSWL SAVKASDAKY EVVTPDEKGN WINQTENNWD DLLPVADKKT KAAKVASQEK
AIFKLFSLGV ATNRDDWVYD FSENDLAAKA KHLVGIYEQD RKRLKTLKSP KEIADALDYS
IKWTRSVKKD LLGRKKHRFN KSSIITANYR PYVKKAMYRD TAFNEMSNLT RTIFGMGQHL
NSSITFMGDS SGKPFLSLAI NQVPDLNFVS PASGGSQTLA RYRYNLEGGR IDNITDWAFN
KFTAHYGKKA KITKDDIFAY VYAVLHDPVY RETYAINLKR EFPRIPFYPD FPQWRDWGQT
LLDLHIGYEK VKPYKLKRSD EEDVKAVKAG LAPKVSLKAD KDAGTITLDS ETTLSGIPPE
CWDYKLGNRS GLEWILDQYK EKTPRDPTIR EKFNTYRFAD YKEQVVDLLK RVTRVSVETM
EVVEKMKAVK RSSNEDSKDD
//
