ID A0A0K2JEH9_SPIKU Unreviewed; 869 AA.
AC A0A0K2JEH9;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460,
GN ECO:0000313|EMBL:ALA96995.1};
GN ORFNames=SKUN_0070 {ECO:0000313|EMBL:ALA96995.1};
OS Spiroplasma kunkelii CR2-3x.
OC Bacteria; Mycoplasmatota; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC Spiroplasma.
OX NCBI_TaxID=273035 {ECO:0000313|EMBL:ALA96995.1, ECO:0000313|Proteomes:UP000062963};
RN [1] {ECO:0000313|EMBL:ALA96995.1, ECO:0000313|Proteomes:UP000062963}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CR2-3x {ECO:0000313|EMBL:ALA96995.1,
RC ECO:0000313|Proteomes:UP000062963};
RX PubMed=26494665;
RA Davis R.E., Shao J., Dally E.L., Zhao Y., Gasparich G.E., Gaynor B.J.,
RA Athey J.C., Harrison N.A., Donofrio N.;
RT "Complete Genome Sequence of Spiroplasma kunkelii Strain CR2-3x, Causal
RT Agent of Corn Stunt Disease in Zea mays L.";
RL Genome Announc. 3:e01216-e01215(2015).
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; CP010899; ALA96995.1; -; Genomic_DNA.
DR RefSeq; WP_053390374.1; NZ_CP010899.1.
DR AlphaFoldDB; A0A0K2JEH9; -.
DR STRING; 273035.SKUN_0070; -.
DR KEGG; skn:SKUN_0070; -.
DR PATRIC; fig|273035.7.peg.84; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000062963; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 2..259
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 626..833
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 869 AA; 100365 MW; 9349726ED5AC3372 CRC64;
MKKVLLIDGN SLLFRAFYAT AYNGEMLKSF DGTPTNAVYA FANMLNKILK TNNYYSVVVA
FDKGKKNFRH DLLFDYKDGR SKTPNELIVQ FPIVKEFLDS YQIPYLEQEG YEADDLLGCL
AVLAEKEDFY VDIFSSDKDL YQLITNKTNI LVPRQGDSAD VIDETALITK WGITPLQVPD
LKGLMGDPSD NLKGVPKVGK KTAIKLIKEY HSIENLYDNI EQIKGALRQN LLNHKESALL
CKKIATIFCD INLEHFTFTH FSPNQEKLMQ FYLKYNMNSF VTKLFNNQDN STENSNITVK
IIEEWCSEFN ENNTTVYLEL LDENYHTAEI IGFGIVNSKN VFYFDYIHAK HDKLWHQFLN
DTKYQKLTYH SKSLIVALAR DNIFVQNIEY DMMLAGYVYN SNAKNSLDTY INLFEKKQIL
TDELFYGKGV KKQIPDDVIK LSHFIGEKAA YIHKLRQKII ELLKSNQQYD LYYKIELPTA
FALARMEING VKVNQDELTT QTLRIEKIVQ ELNNEINDIA QKEINPNSPK QISELLFQDL
SLPDRKKGST AQEALEKIKN NHVIIPNILD HRKYKKLYST YLKGMEKYIF ADGKVHTIYK
QTLTNTGRLS SVEPNMQNIS IRDDVQKEVR KIFVVSSPNN VLLSCDYSQI ELRILAHMSK
DQDLIAAFNK NEDIHTNTAM KIFNLSKNEI TPNIRRSAKA VNFGIIYGIS DFGLATDLNI
SVAKAKAIIN NYYQQFPTIK VFIDKQVEFC KQNGYVTTIF NRKRYVPEIN DRNYMQREFG
KRIAINMPIQ GSAADIIKIA LKNIDQEFLK LNLKAKIIAQ IHDELIFEIP QVELIQVQKI
VKTLMEDSTK LDVKLIVDMK TGLSWYDLK
//
