ID   A0A0K2YI02_9NOCA        Unreviewed;       560 AA.
AC   A0A0K2YI02;
DT   11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT   11-NOV-2015, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   SubName: Full=3-oxosteroid 1-dehydrogenase {ECO:0000313|EMBL:CRK51657.1};
DE            EC=1.3.99.4 {ECO:0000313|EMBL:CRK51657.1};
GN   Name=ksdD {ECO:0000313|EMBL:CRK51657.1};
GN   ORFNames=RHCRD62_30321 {ECO:0000313|EMBL:CRK51657.1};
OS   Rhodococcus sp. RD6.2.
OC   Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC   Rhodococcus.
OX   NCBI_TaxID=260936 {ECO:0000313|EMBL:CRK51657.1, ECO:0000313|Proteomes:UP000044872};
RN   [1] {ECO:0000313|EMBL:CRK51657.1, ECO:0000313|Proteomes:UP000044872}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RD6.2 {ECO:0000313|EMBL:CRK51657.1,
RC   ECO:0000313|Proteomes:UP000044872};
RA   Wang D.B., Wang M.;
RL   Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
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DR   EMBL; CVQP01000006; CRK51657.1; -; Genomic_DNA.
DR   RefSeq; WP_050064853.1; NZ_CVQP01000006.1.
DR   AlphaFoldDB; A0A0K2YI02; -.
DR   STRING; 260936.RHCRD62_30321; -.
DR   OrthoDB; 9813348at2; -.
DR   Proteomes; UP000044872; Unassembled WGS sequence.
DR   GO; GO:0047571; F:3-oxosteroid 1-dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProt.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   InterPro; IPR003953; FAD-binding_2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR   PANTHER; PTHR43400:SF7; FAD_BINDING_2 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR43400; FUMARATE REDUCTASE; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   PRINTS; PR00368; FADPNR.
DR   PRINTS; PR00411; PNDRDTASEI.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   SUPFAM; SSF56425; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
PE   4: Predicted;
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000313|EMBL:CRK51657.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000044872}.
FT   DOMAIN          13..542
FT                   /note="FAD-dependent oxidoreductase 2 FAD binding"
FT                   /evidence="ECO:0000259|Pfam:PF00890"
SQ   SEQUENCE   560 AA;  59278 MW;  50AC06CA95144537 CRC64;
     MTHAHEQVHE TVDLVVVGSG AAGMAAAITA ARSGLDTVIV EKSEYWGGSS ARSGGGVWIP
     GNSVLARQAP TDDLTDARTY LTSIVGEGAD SARIDAYIDR GAEALDFLIE HSALDLEWVK
     GYSDYFPEAP GGRATGRSCE PVPFDGRRLG DDLRTLHPSY TKAPLGVVVK QGDYRWLSTG
     LRHWRGPVRM IGVGARTFLG RVRGKKLLGL GSALMGQMML GVRAAGVPVR LGTAMAGLVI
     EGGRVVGIEV ERDGVRTVLR ARRGVVLACG GFDNNAEMRQ LYQREPVNTS WSLGAPTNTG
     DGITAGLEAG ATVSYMDDAW WAPSIPLPKG PWFALAERSL PRSLIVNDRG VRFMNESLPY
     VEATHRMFGG PFGQGEGPAE NFPAWLVFDQ TYRDRYMFAG LPARQPMPRS WQRDGAIVAA
     GSIAELADRI GVPADALTAT VDRFNGHARA GSDPDFGRGQ SAYDHYYGDI TNKPNPSLGE
     LTKAPFYAAQ MVPGDLGTKG GIDTDAVGRA LRGDGTVIDG LYAAGNTSAP VMGHTYAGPG
     ATIGPAIVFG YLAALDAAAG
//