ID A0A0K2YKI6_9NOCA Unreviewed; 430 AA.
AC A0A0K2YKI6;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE SubName: Full=Ni-dependent hydrogenase large subunit {ECO:0000313|EMBL:CRK51230.1};
GN ORFNames=RHCRD62_20669 {ECO:0000313|EMBL:CRK51230.1};
OS Rhodococcus sp. RD6.2.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Rhodococcus.
OX NCBI_TaxID=260936 {ECO:0000313|EMBL:CRK51230.1, ECO:0000313|Proteomes:UP000044872};
RN [1] {ECO:0000313|EMBL:CRK51230.1, ECO:0000313|Proteomes:UP000044872}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RD6.2 {ECO:0000313|EMBL:CRK51230.1,
RC ECO:0000313|Proteomes:UP000044872};
RA Wang D.B., Wang M.;
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
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DR EMBL; CVQP01000005; CRK51230.1; -; Genomic_DNA.
DR RefSeq; WP_050064432.1; NZ_CVQP01000005.1.
DR AlphaFoldDB; A0A0K2YKI6; -.
DR STRING; 260936.RHCRD62_20669; -.
DR OrthoDB; 9761717at2; -.
DR Proteomes; UP000044872; Unassembled WGS sequence.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR43600; COENZYME F420 HYDROGENASE, SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43600:SF2; CYTOSOLIC NIFE-HYDROGENASE, ALPHA SUBUNIT; 1.
DR Pfam; PF00374; NiFeSe_Hases; 2.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|PIRSR:PIRSR601501-1};
KW Reference proteome {ECO:0000313|Proteomes:UP000044872}.
FT BINDING 46
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 65
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 68
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 68
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 371
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 415
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 418
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 421
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 430 AA; 47646 MW; C4D7F6E1438A46BA CRC64;
MGDEDRRLIV RGLARVEGEG RLRVAVRGDR VERAELSIYE PPRFFESFLR GRDFREPPDI
TSRICGICPV AYQVSACNAI EDACGAQPDD AVTRLRRLLY CGEWIASHVL HIYFLHAPDF
AGVDDSIALA LADRPRVERG LDLKKAGNAI LELLGGRPIH PVNVRIGGFY RTPTREELRP
LAEQLRRALD DALDTAAWIG GFDFPDHELP YVMIALTRPD RYAIESGAIH TTGGTRFSAG
EFPEHVTEHQ VPHSTALHAR LDGHRYLAGP LARYTLNSAR LSETARQAAS AAGLGEQCRN
PFRSIAVRAV EVVYAVDEAL RLVESYEQPS APARVLHPRA ATGHGVSEAP RGLLYHRYDL
DGDGTIRSAT IVPPTSQNQS AIEHELAGVV ERNLHLDDAD LTMLCERTIR NHDPCISCAT
HFLDLTIDRQ
//
