ID A0A0K3C7F0_RHOTO Unreviewed; 499 AA.
AC A0A0K3C7F0;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=tRNA (guanine(9)-N1)-methyltransferase {ECO:0000256|ARBA:ARBA00020451};
DE EC=2.1.1.221 {ECO:0000256|ARBA:ARBA00012797};
DE AltName: Full=tRNA methyltransferase 10 {ECO:0000256|ARBA:ARBA00032166};
DE AltName: Full=tRNA(m1G9)-methyltransferase {ECO:0000256|ARBA:ARBA00031792};
DE Flags: Fragment;
GN Name=FGENESH: predicted gene_2.659 {ECO:0000313|EMBL:CTR05629.1};
GN ORFNames=BN2166_0014900 {ECO:0000313|EMBL:CTR05629.1};
OS Rhodotorula toruloides (Yeast) (Rhodosporidium toruloides).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Microbotryomycetes; Sporidiobolales; Sporidiobolaceae; Rhodotorula.
OX NCBI_TaxID=5286 {ECO:0000313|EMBL:CTR05629.1, ECO:0000313|Proteomes:UP000199069};
RN [1] {ECO:0000313|EMBL:CTR05629.1, ECO:0000313|Proteomes:UP000199069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Single colony {ECO:0000313|EMBL:CTR05629.1};
RA Cajimat M.N.B., Milazzo M.L., Fulhorst C.F.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221;
CC Evidence={ECO:0000256|ARBA:ARBA00000855};
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DR EMBL; CWKI01000002; CTR05629.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0K3C7F0; -.
DR STRING; 5286.A0A0K3C7F0; -.
DR OMA; TAWTETR; -.
DR Proteomes; UP000199069; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd18089; SPOUT_Trm10-like; 1.
DR Gene3D; 3.40.1280.30; -; 1.
DR InterPro; IPR028564; MT_TRM10-typ.
DR InterPro; IPR038459; MT_TRM10-typ_sf.
DR InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR PANTHER; PTHR13563; TRNA (GUANINE-9-) METHYLTRANSFERASE; 1.
DR PANTHER; PTHR13563:SF13; TRNA METHYLTRANSFERASE 10 HOMOLOG A; 1.
DR Pfam; PF01746; tRNA_m1G_MT; 1.
DR PROSITE; PS51675; SAM_MT_TRM10; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000199069};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 151..398
FT /note="SAM-dependent MTase TRM10-type"
FT /evidence="ECO:0000259|PROSITE:PS51675"
FT REGION 43..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 249..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 397..428
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 397..417
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:CTR05629.1"
SQ SEQUENCE 499 AA; 54141 MW; 048172AED94F6201 CRC64;
DWHALAVLRV QKNQILVDPD ADSQEPLVVP LTSLRANPLA CRAMSTSTPL PPSIDQASAP
ASTSGPAANG AAPTSEALTA PSADAQLTAS TSTSTLPPGI TKSALKRQRK QEAFQAKKLV
KRQQEKERKK AKAAEIKRLV AEGVMEKPES KKRKKAGGEP HGARIVIDMG FDELMTEKEV
KSMASQLAYC YSANRASPHP FPILVTSFNG RLREGYDKRG DFKSWKGVEW WDDGVEKLWD
GVREVAPADE AAETATSAGE ASSAEAPVAS TPSIPLNTLG VFSGQPCTRV PRSTVTYLTG
DSPNVLTSLT PGHTYILGGI VDRNRYKNLC LNKANSLGIA HAQLPIGEYL PEMQTRKVLT
VNQVFEILVN WVEAGEKDGK EGWGEALRKV MPERKFDPDA RKKRREARKK AGEAVSASED
EGEIGQADAG VYVAEDEDEG EEVQMVVDKV DEVRGEAEVA EARKEAEAVV SNVDAEEVQG
LQQQHGRYGT ARAVVIYRF
//
