ID A0A0K3CMW0_RHOTO Unreviewed; 779 AA.
AC A0A0K3CMW0;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=cysteine--tRNA ligase {ECO:0000256|ARBA:ARBA00012832};
DE EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN Name=FGENESH: predicted gene_11.147 {ECO:0000313|EMBL:CTR09790.1};
GN ORFNames=AAT19DRAFT_9855 {ECO:0000313|EMBL:PRQ71740.1}, BN2166_0056510
GN {ECO:0000313|EMBL:CTR09790.1};
OS Rhodotorula toruloides (Yeast) (Rhodosporidium toruloides).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Microbotryomycetes; Sporidiobolales; Sporidiobolaceae; Rhodotorula.
OX NCBI_TaxID=5286 {ECO:0000313|EMBL:CTR09790.1, ECO:0000313|Proteomes:UP000199069};
RN [1] {ECO:0000313|EMBL:CTR09790.1, ECO:0000313|Proteomes:UP000199069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Single colony {ECO:0000313|EMBL:CTR09790.1};
RA Cajimat M.N.B., Milazzo M.L., Fulhorst C.F.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:PRQ71740.1, ECO:0000313|Proteomes:UP000239560}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 0880 {ECO:0000313|EMBL:PRQ71740.1,
RC ECO:0000313|Proteomes:UP000239560};
RX PubMed=29521624;
RA Coradetti S.T., Pinel D., Geiselman G., Ito M., Mondo S., Reilly M.C.,
RA Cheng Y.F., Bauer S., Grigoriev I., Gladden J.M., Simmons B.A., Brem R.,
RA Arkin A.P., Skerker J.M.;
RT "Functional genomics of lipid metabolism in the oleaginous yeast
RT Rhodosporidium toruloides.";
RL Elife 7:0-0(2018).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
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DR EMBL; CWKI01000011; CTR09790.1; -; Genomic_DNA.
DR EMBL; LCTV02000011; PRQ71740.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0K3CMW0; -.
DR STRING; 5286.A0A0K3CMW0; -.
DR OMA; FHNDMKS; -.
DR OrthoDB; 2140072at2759; -.
DR Proteomes; UP000199069; Unassembled WGS sequence.
DR Proteomes; UP000239560; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00672; CysRS_core; 1.
DR Gene3D; 1.20.120.1910; Cysteine-tRNA ligase, C-terminal anti-codon recognition domain; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR InterPro; IPR015803; Cys-tRNA-ligase.
DR InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00435; cysS; 1.
DR PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR Pfam; PF01406; tRNA-synt_1e; 1.
DR PRINTS; PR00983; TRNASYNTHCYS.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000313|EMBL:PRQ71740.1}; ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000199069};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 23..474
FT /note="tRNA synthetases class I catalytic"
FT /evidence="ECO:0000259|Pfam:PF01406"
FT REGION 711..761
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 736..761
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 779 AA; 86520 MW; 7B0EDF8400AD4526 CRC64;
MQNSWRIPQP SAPEPKLKVD FVTKKPGIVT WYNCGPTVYD ASHMGHARNY MAQDIMRRIL
RDYFGYEVNF VMNITDVDDK IIVRARQSHL LKKYLAELAT SSTPLSSRVL QEVSTAWTAY
FTKAFAASLP SPVAADDFAA AEAGWAQVQD LAKDANWVEA QKTANEKFPM YFAAVEAGYT
ALKLAQKQEA AGATGLTDAE ALIEANKDAV GLWLDKQLGA SVTDPAVFRD LAAFWEDSYF
KSMAALHVER PTTLTRVSEY IPEIVTFVEG IVKRGFAYEG GGSVWFDTVK FEGAKGEGEG
DDEWRHTYAK LQPWSKGNRE LLEDGEGSLT STTGKRSAAD FALWKSSKPG EPAWPSPWGP
GRPGWHIECS VMASAVLGEG MDVHSGGVDL AFPHHDNEIA QSEAFHNCRQ WVNYFLHTGH
LHIEGLKMSK SLKNFITIDD ALEKHTARQL RFAFLSQNWN ARLDFKESSM QEVKSAETVL
NNFFTNVKAL ATEAKERSVA SDGQHHYEQA EKDLLAQLEQ AQLAFRTALC DSFDTPTALQ
ILLDIVSKTN IYLARGRRQV NFAVILAVSE WVTRMLRMFG LGEGSPVDGN GDKVIGWGVA
AAKGDEQSVD RESILMPYLR ALSGFRDQVR QLAMSGAASS DILALTDKLR DHDLVDLGVA
LDDQEDGRAM VKLVPPEILR EARDAKAAAV REKAAKKAAA AAAAEAKRLE RLEKGRVPPT
EMFRSNTAEY SAWDEQGLPT KDKDGNELPK SRGKKLKKEW DMQKKLHDEF LKEQEKQSA
//
