ID A0A0K8P7I6_IDESA Unreviewed; 547 AA.
AC A0A0K8P7I6;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE SubName: Full=Mg-protoporphyrin IX monomethyl ester oxidative cyclase, anaerobic {ECO:0000313|EMBL:GAP38618.1};
DE EC=1.14.13.81 {ECO:0000313|EMBL:GAP38618.1};
GN ORFNames=ISF6_5171 {ECO:0000313|EMBL:GAP38618.1};
OS Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Sphaerotilaceae; Piscinibacter.
OX NCBI_TaxID=1547922 {ECO:0000313|EMBL:GAP38618.1, ECO:0000313|Proteomes:UP000037660};
RN [1] {ECO:0000313|Proteomes:UP000037660}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 110686 / TISTR 2288 / 201-F6
RC {ECO:0000313|Proteomes:UP000037660};
RA Yoshida S., Hiraga K., Takehana T., Taniguchi I., Yamaji H., Maeda Y.,
RA Toyohara K., Miyamoto K., Kimura Y., Oda K.;
RT "Discovery of a poly(ethylene terephthalate assimilation.";
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GAP38618.1, ECO:0000313|Proteomes:UP000037660}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 110686 / TISTR 2288 / 201-F6
RC {ECO:0000313|Proteomes:UP000037660};
RX PubMed=26965627; DOI=10.1126/science.aad6359;
RA Yoshida S., Hiraga K., Takehana T., Taniguchi I., Yamaji H., Maeda Y.,
RA Toyohara K., Miyamoto K., Kimura Y., Oda K.;
RT "A bacterium that degrades and assimilates poly(ethylene terephthalate).";
RL Science 351:1196-1199(2016).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAP38618.1}.
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DR EMBL; BBYR01000082; GAP38618.1; -; Genomic_DNA.
DR RefSeq; WP_054022476.1; NZ_BBYR01000082.1.
DR AlphaFoldDB; A0A0K8P7I6; -.
DR STRING; 1547922.ISF6_5171; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000037660; Unassembled WGS sequence.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR NCBIfam; TIGR02026; BchE; 1.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00302; anaerobic_magnesium-protoporph; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Oxidoreductase {ECO:0000313|EMBL:GAP38618.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000037660}.
FT DOMAIN 9..143
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 190..414
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 547 AA; 62530 MW; 32C4300464D7865E CRC64;
MRILLLHPNY HSGGAEIAGN WPPAWVACLS GYLKAAGYTE VAFVDAMTHH LDDEQVRERL
RAFRPDVVGC TAITPAIYQA ERLLQVAKEV DPRIVTVLGG IHGTFMYPQV LKEAPWIDAV
VRGEGEQVFL NLVSAVDEGR FARDPGSVRG IAYRAGDEVV ASPAEPTIQD LDRIPPDWGI
LEWEHYRYVP LGVRVAIPNF ARGCPFTCSF CSQWKFWRDY RVRDPVKVVD EIEALVRDHG
IGFFILADEE PTIHRRKFVQ FCEELERRRL PVQWGINTRV TDILRDEALL PLYRRAGLVH
VSLGTEAAAQ LKLDRFHKET TVAQNKKAIR LLREAGIVSE AQFIVGLENE TPETLEETYR
MARDWNPDLA NWAMYTPWPF SDLFQELGDK VEVFDFAKYN FVTPIMKPDA MDRAELLDRV
MHNYRRFFMN KTFLQYPWTR DRARRSYLMG CLKAFVKSGF QRRFYDLGRV GYWGPQSRRT
VDFGFDGTRR FERPTAEAVA AGDDGWVTMH GPKIAQRRRR GELNAQQAIA ACGGGTEQLA
DDVATLP
//
