ID A0A0K8QAW1_9MICC Unreviewed; 461 AA.
AC A0A0K8QAW1;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE Flags: Fragment;
GN ORFNames=AHiyo6_37560 {ECO:0000313|EMBL:GAP57191.1};
OS Arthrobacter sp. Hiyo6.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Micrococcaceae;
OC Arthrobacter.
OX NCBI_TaxID=1588022 {ECO:0000313|EMBL:GAP57191.1, ECO:0000313|Proteomes:UP000037466};
RN [1] {ECO:0000313|Proteomes:UP000037466}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hiyo6 {ECO:0000313|Proteomes:UP000037466};
RA Hiraoka S., Machiyama A., Iwasaki W.;
RT "Microbial genome analysis of tsunami-affected soil.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GAP57191.1, ECO:0000313|Proteomes:UP000037466}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hiyo6 {ECO:0000313|EMBL:GAP57191.1,
RC ECO:0000313|Proteomes:UP000037466};
RX PubMed=26764021; DOI=10.1186/s12864-016-2380-4;
RA Hiraoka S., Machiyama A., Ijichi M., Inoue K., Oshima K., Hattori M.,
RA Yoshizawa S., Kogure K., Iwasaki W.;
RT "Genomic and metagenomic analysis of microbes in a soil environment
RT affected by the 2011 Great East Japan Earthquake tsunami.";
RL BMC Genomics 17:53-53(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAP57191.1}.
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DR EMBL; BBUE01000627; GAP57191.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0K8QAW1; -.
DR Proteomes; UP000037466; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 39..323
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 324..461
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 60..67
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
FT NON_TER 461
FT /evidence="ECO:0000313|EMBL:GAP57191.1"
SQ SEQUENCE 461 AA; 51853 MW; 3864D39FD2C69FD3 CRC64;
MAGARRFPGR IRRWRRSKRR ANSRGGDAPG LPEAAELLRG LNPQQEEAVK HSGSALLIVA
GAGSGKTRVL SNRIAYLMAT GRAHHGEILA ITFTNKAAAE MRERIEALVG ARAKNMWIST
FHSSCVRILR REAPNVGLKS NFSIYDSADS LRLVTQVSKS LDLDPKKFAP KAIQHKISAL
KNELIDADSY ALSANHNDPF ESAVADVFKG YTQRLRQANA MDFDDLIAET VYMFRAFPAL
AESYRRRFRH VLVDEYQDTN HAQYALVREI VGEGPNASEL TVVGDSDQSI YAFRGADIRN
IVEFEKDYPN ARTIKLEQNY RSTQNILTAA NSVISRNPNR PEKRLWTAEG DGHKIIGYVG
ENEHDEAQFI AKEIDRLQDE DNLRPGDVAI FYRTNAQSRS IEDVLVRVGL PYKVVGGTRF
YERKEIKDAL AYLRVLVNPD DDVNLRRVLN EPKRGIGDRA E
//
