ID A0A0K9YZU7_9BACL Unreviewed; 790 AA.
AC A0A0K9YZU7;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE SubName: Full=Competence protein ComEC {ECO:0000313|EMBL:KNB74186.1};
GN ORFNames=ADS79_08970 {ECO:0000313|EMBL:KNB74186.1};
OS Brevibacillus reuszeri.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus.
OX NCBI_TaxID=54915 {ECO:0000313|EMBL:KNB74186.1, ECO:0000313|Proteomes:UP000036834};
RN [1] {ECO:0000313|Proteomes:UP000036834}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 9887 {ECO:0000313|Proteomes:UP000036834};
RA Liu B., Wang J., Zhu Y., Liu G., Chen Q., Chen Z., Lan J., Che J., Ge C.,
RA Shi H., Pan Z., Liu X.;
RT "Genome sequencing project for genomic taxonomy and phylogenomics of
RT Bacillus-like bacteria.";
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Counteracts the endogenous Pycsar antiviral defense system.
CC Phosphodiesterase that enables metal-dependent hydrolysis of host
CC cyclic nucleotide Pycsar defense signals such as cCMP and cUMP.
CC {ECO:0000256|ARBA:ARBA00034301}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3',5'-cyclic CMP + H2O = CMP + H(+); Xref=Rhea:RHEA:72675,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58003,
CC ChEBI:CHEBI:60377; Evidence={ECO:0000256|ARBA:ARBA00034221};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72676;
CC Evidence={ECO:0000256|ARBA:ARBA00034221};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3',5'-cyclic UMP + H2O = H(+) + UMP; Xref=Rhea:RHEA:70575,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57865,
CC ChEBI:CHEBI:184387; Evidence={ECO:0000256|ARBA:ARBA00034227};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70576;
CC Evidence={ECO:0000256|ARBA:ARBA00034227};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KNB74186.1}.
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DR EMBL; LGIQ01000005; KNB74186.1; -; Genomic_DNA.
DR RefSeq; WP_049738187.1; NZ_LGIQ01000005.1.
DR AlphaFoldDB; A0A0K9YZU7; -.
DR STRING; 54915.ADS79_08970; -.
DR PATRIC; fig|54915.3.peg.7256; -.
DR OrthoDB; 9761531at2; -.
DR Proteomes; UP000036834; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0030420; P:establishment of competence for transformation; IEA:InterPro.
DR CDD; cd07731; ComA-like_MBL-fold; 1.
DR Gene3D; 3.60.15.10; Ribonuclease Z/Hydroxyacylglutathione hydrolase-like; 1.
DR InterPro; IPR035681; ComA-like_MBL.
DR InterPro; IPR004477; ComEC_N.
DR InterPro; IPR004797; Competence_ComEC/Rec2.
DR InterPro; IPR025405; DUF4131.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR NCBIfam; TIGR00360; ComEC_N-term; 1.
DR NCBIfam; TIGR00361; ComEC_Rec2; 1.
DR PANTHER; PTHR30619; DNA INTERNALIZATION/COMPETENCE PROTEIN COMEC/REC2; 1.
DR PANTHER; PTHR30619:SF1; RECOMBINATION PROTEIN 2; 1.
DR Pfam; PF03772; Competence; 1.
DR Pfam; PF13567; DUF4131; 1.
DR Pfam; PF00753; Lactamase_B; 1.
DR SMART; SM00849; Lactamase_B; 1.
DR SUPFAM; SSF56281; Metallo-hydrolase/oxidoreductase; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 20..38
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 45..62
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 240..266
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 278..304
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 347..366
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 404..430
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 470..487
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 499..516
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 531..741
FT /note="Metallo-beta-lactamase"
FT /evidence="ECO:0000259|SMART:SM00849"
SQ SEQUENCE 790 AA; 87989 MW; 7549C5F4112E66DE CRC64;
MLGASILMMA GYLLSAYLHP AWLLAAAGLS AALAVVIPQA LRKQCVFYAL ILILAGLYFN
GYEFLHRSEM KQLAETEETI RIRAMIASPV KRDGDVARFF VDVQQIEDRY GVEQRLQHTE
RIVLRVKLAT FEQAGLIEKW RSGSVVTAKI KLSLPSPARN PHAFDYAQYL YWQGVHVTGE
AEFKGMEHQT QNTIMARFQE WQGDGANRIE RLFPDPEVAG YMKSLLLGLG QEVTPELSDM
YSFLGLSHIL AISGLHVTLV SSMFMWTLER TGIRRKTALI TTICMLIGYV LLVGASASAV
RSGIMGGVGL ACQILGKRLD GREVWAGALL VMLLFNPYQL WHIGFQLSFA VTLGLIIYVP
FSLQMWSWGP VWLRSLIAVT LVAQVVSFPF LIYHFHQFSP LSWLVNLLVT PLLSIIVLPL
GYIAIILGMV HPALAMWPVK LSSLILDGVH QPLFGLQKLE IPFTYWQHPP WWLLFLYTIF
LIVIPFLWKR GFHRRRDTIL YIAIFFALLL AARQPFSGEK EVRITFLDVG QGDSIVVEIA
NQKVYLIDAG GTISFAAREP WREKRNPFEV GKDVVLPFLM ARGIEKIDRV VMTHGDMDHI
GGLAALVPRF AIGEVIVNGS TPKGYEQEIL QLVREKRVPI VTGSPGQTWS DAPGIEWKWL
HPGQTSAYTG NDSSVVLQLT AYETIVLFTG DVERNGEFRL VKGGLPPVDV LKVAHHGSKT
SSTEALLSAI QPKAAVISAG ANNRYGHPSP EVLTRLKNAG SSVYRTDQQG AVTLIITTSG
MSWKTQLTDT
//
