ID A0A0L0BPT7_LUCCU Unreviewed; 1053 AA.
AC A0A0L0BPT7;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Luciferin 4-monooxygenase {ECO:0000256|ARBA:ARBA00019043};
DE EC=1.13.12.7 {ECO:0000256|ARBA:ARBA00012532};
GN ORFNames=FF38_14204 {ECO:0000313|EMBL:KNC22021.1};
OS Lucilia cuprina (Green bottle fly) (Australian sheep blowfly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC Calliphoridae; Luciliinae; Lucilia.
OX NCBI_TaxID=7375 {ECO:0000313|EMBL:KNC22021.1, ECO:0000313|Proteomes:UP000037069};
RN [1] {ECO:0000313|EMBL:KNC22021.1, ECO:0000313|Proteomes:UP000037069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LS {ECO:0000313|EMBL:KNC22021.1,
RC ECO:0000313|Proteomes:UP000037069};
RC TISSUE=Full body {ECO:0000313|EMBL:KNC22021.1};
RX PubMed=26108605; DOI=10.1038/ncomms8344;
RA Anstead C.A., Korhonen P.K., Young N.D., Hall R.S., Jex A.R., Murali S.C.,
RA Hughes D.S., Lee S.F., Perry T., Stroehlein A.J., Ansell B.R.,
RA Breugelmans B., Hofmann A., Qu J., Dugan S., Lee S.L., Chao H., Dinh H.,
RA Han Y., Doddapaneni H.V., Worley K.C., Muzny D.M., Ioannidis P.,
RA Waterhouse R.M., Zdobnov E.M., James P.J., Bagnall N.H., Kotze A.C.,
RA Gibbs R.A., Richards S., Batterham P., Gasser R.B.;
RT "Lucilia cuprina genome unlocks parasitic fly biology to underpin future
RT interventions.";
RL Nat. Commun. 6:7344-7344(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + firefly D-luciferin + O2 = AMP + CO2 + diphosphate +
CC firefly oxyluciferin + hnu; Xref=Rhea:RHEA:10732, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16792, ChEBI:CHEBI:30212,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58038,
CC ChEBI:CHEBI:456215; EC=1.13.12.7;
CC Evidence={ECO:0000256|ARBA:ARBA00001278};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000256|ARBA:ARBA00004275}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000256|ARBA:ARBA00006432}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KNC22021.1}.
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DR EMBL; JRES01001565; KNC22021.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0L0BPT7; -.
DR STRING; 7375.A0A0L0BPT7; -.
DR EnsemblMetazoa; KNC22021; KNC22021; FF38_14204.
DR OMA; CDENVYE; -.
DR Proteomes; UP000037069; Unassembled WGS sequence.
DR GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR Gene3D; 3.30.300.30; -; 2.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 2.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR PANTHER; PTHR24096:SF353; GH16244P-RELATED; 1.
DR PANTHER; PTHR24096; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 2.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 3: Inferred from homology;
KW Luminescence {ECO:0000256|ARBA:ARBA00023223};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023033};
KW Peroxisome {ECO:0000256|ARBA:ARBA00023140};
KW Photoprotein {ECO:0000256|ARBA:ARBA00023262};
KW Reference proteome {ECO:0000313|Proteomes:UP000037069}.
FT DOMAIN 49..385
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 443..518
FT /note="AMP-binding enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13193"
FT DOMAIN 561..905
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 957..1033
FT /note="AMP-binding enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13193"
SQ SEQUENCE 1053 AA; 118268 MW; 804EF56D24B664A6 CRC64;
MYDYLCQYDK DQKLWTSAKR HEFYNENVSA GELAYFVMRN TNPSEVMQIS DSDGTVMTYG
SALTTAIRIA QHFDTIGLTE DDIVSIFAPN TTYVMPVALA AWFNGTPFQP MNTVMETNVC
TQIFEKIQPK IIFCDGLHYE KLKKAAAAFK PAIYTICNHV ENVPRIEELL EPTKSERNFK
PKPLTKGPTQ TMIIMLSSGT TGLPKGICLS NRYVISDMAF TYQYDTIFST SSIDWLTGLV
TLLTNVLVGG TRVITSKPYS AEYLHEIITK YKISVLVTNP SAMLELTSLP IYSKECMKSI
RMLIMGGSSC PENTLKIIRS SLTNGKLYFG YGSTEMGAIA FNNRDYKLKS VGQILPNLQM
KIVDSTTGER LGPNEKGEVC LRKTNDQWLG YYKNPEANKS TIDAEGFVHT GDLGYVDDEH
YLYLVDRCRD VMKYWGYKWS PHEIEVLVAE MPEVKEVCAF GVYDNVKLYV PAAAVVVKPG
CSLTSKDVVE FMERRSEVHY KHLDAGAFIV NELPRNRNDM ADFITIYHQE EKLWKGLDKP
KLCNDNISAG AKALEVLRSK NPQDIMQISD SEKTTITYGS ALKAAIKIAE YFKSLSLNAD
DVVGILAPDT TYVMPVALAS WFNGTPFQAI NCKWETAVVR NLYEFSRPKI IFCDGLQYEK
VKAACENFKP LIYTLCNHLE NVPQIEDLLQ RASGAKPLKY GSKQTMVIMV SSGTSGMPKG
VCVPNSTCIT DFGFTNDTTK MFSTSSIDWS SGLLTFMANV LVGGTRIITQ QPFTAEYLLQ
LIVKYKINFL STNPSNLIQA SLLKEYTPET MQSVKLIFTA GSYCSELNLE RMRSALTKGL
LFYGYGGTET GGVAGRLNSN KPKSVGRLLP NVQLKIVDVN NGEHLGPNEI GEICVRHDYE
WCGYYNNAKA TANTLDSEGF IHTGDLGYMD EDNFLFLTGR CKDIMKYLGF QYSPNDIEEI
IAEMEDVVDV CVFGVYDEVE QDVAAAAVVK REGSTLTKQQ VIKYVESKTE VQHKRIHYGI
FFVKELPRNH NGKLLRDKIK KICLEMERCD KNE
//
