ID A0A0L0C605_LUCCU Unreviewed; 1498 AA.
AC A0A0L0C605;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=HECT domain-containing protein {ECO:0000259|PROSITE:PS50237};
DE Flags: Fragment;
GN ORFNames=FF38_11339 {ECO:0000313|EMBL:KNC27715.1};
OS Lucilia cuprina (Green bottle fly) (Australian sheep blowfly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC Calliphoridae; Luciliinae; Lucilia.
OX NCBI_TaxID=7375 {ECO:0000313|EMBL:KNC27715.1, ECO:0000313|Proteomes:UP000037069};
RN [1] {ECO:0000313|EMBL:KNC27715.1, ECO:0000313|Proteomes:UP000037069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LS {ECO:0000313|EMBL:KNC27715.1,
RC ECO:0000313|Proteomes:UP000037069};
RC TISSUE=Full body {ECO:0000313|EMBL:KNC27715.1};
RX PubMed=26108605; DOI=10.1038/ncomms8344;
RA Anstead C.A., Korhonen P.K., Young N.D., Hall R.S., Jex A.R., Murali S.C.,
RA Hughes D.S., Lee S.F., Perry T., Stroehlein A.J., Ansell B.R.,
RA Breugelmans B., Hofmann A., Qu J., Dugan S., Lee S.L., Chao H., Dinh H.,
RA Han Y., Doddapaneni H.V., Worley K.C., Muzny D.M., Ioannidis P.,
RA Waterhouse R.M., Zdobnov E.M., James P.J., Bagnall N.H., Kotze A.C.,
RA Gibbs R.A., Richards S., Batterham P., Gasser R.B.;
RT "Lucilia cuprina genome unlocks parasitic fly biology to underpin future
RT interventions.";
RL Nat. Commun. 6:7344-7344(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside +
CC phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000815};
CC -!- SIMILARITY: Belongs to the pyrimidine 5'-nucleotidase family.
CC {ECO:0000256|ARBA:ARBA00008389}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KNC27715.1}.
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DR EMBL; JRES01000857; KNC27715.1; -; Genomic_DNA.
DR STRING; 7375.A0A0L0C605; -.
DR EnsemblMetazoa; KNC27715; KNC27715; FF38_11339.
DR OMA; MRVQGHL; -.
DR Proteomes; UP000037069; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR GO; GO:0106411; F:XMP 5'-nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0009117; P:nucleotide metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0000209; P:protein polyubiquitination; IEA:InterPro.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:UniProt.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.10.150.340; Pyrimidine 5'-nucleotidase (UMPH-1), N-terminal domain; 1.
DR InterPro; IPR044611; E3A/B/C-like.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR006434; Pyrimidine_nucleotidase_eu.
DR NCBIfam; TIGR01544; HAD-SF-IE; 1.
DR PANTHER; PTHR45700; UBIQUITIN-PROTEIN LIGASE E3C; 1.
DR PANTHER; PTHR45700:SF2; UBIQUITIN-PROTEIN LIGASE E3C; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF05822; UMPH-1; 1.
DR SFLD; SFLDG01128; C1.4:_5'-Nucleotidase_Like; 1.
DR SFLD; SFLDS00003; Haloacid_Dehalogenase; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50096; IQ; 1.
PE 3: Inferred from homology;
KW Nucleotide metabolism {ECO:0000256|ARBA:ARBA00023080};
KW Reference proteome {ECO:0000313|Proteomes:UP000037069};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1161..1498
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 677..698
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 750..788
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1466
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KNC27715.1"
SQ SEQUENCE 1498 AA; 171805 MW; F4B1AFD90246E019 CRC64;
AAAPPIFTRI LDRIMMGSST EMNQQEDNTM DEISIRLQDI KQLCDDHCKI KDPARVERIL
NEFVRGGTER LQLVSDFDYT ITKQRTADGK PVLSSFGILN ACKSLPANFI EESDKLYHKY
RPIEIDPHIP TKDKIKHMID WWTKTGELLV GFPLGQSEID GIAGKYKDAL RDRTNVLFET
LNRLDIPVLV FSAGLGNSVI SVLQQANVMY SNVKVVSNFL QYKDGLLNGF QQPMIHTFNK
NETALDGSEY YDLVHSRDHI IVMGDSIGDA DMANGVPSSS HIIKIGFLFD HPEQNLERYM
NAFDIVLIDD QTMDVPLALL SAIENFQQRL QSNSTSKNEA QIKKGQNHYK KIMFGFDGEY
RRKPSQSFGG ASTSSDRETI IRKAAEERLK RNQMRRENNG AIVLQSYLRS FIHRQRRKRY
ERETFDEFLR THRDKLTQDE NLAFLLKRII FFYNTKETKD GERLIDICQH VLKNPARLVQ
NSTTDAVWLH RLKKLLDLCI IQLTSVQQTS QAIPLRMLET FTAETSIERY VENEQLIQTY
LDRIFRYLVD KDYYKRLREL LEQKCPPLDG ETLHPPNQFS EAIYQLMLRP LLLANKAASG
FSYGNIICQS FTKHILALPY TDCIRYFLLP CFAECSEFPF EYLLKTLQQC TRTQLPEKMQ
IDSSPVVTFT GASAAESLNT TSNSSSPSSN KGMEDKSQEP TKIFSTFLLH SLLMLDRKQL
KSLHAKSLLG DYIKVIAEVS PKILQLPKSS LKHTGHHQRY HPHASEDTSS DDDSEDEEEE
DQYQNKRNLQ SMDTVDVMTM SIPLSEIEKD SLLECIQLLN EGERVHSILD YIEEYLKDED
VLYGLCEICH NLMIYNKMAM FEYKLLYTLA FMPNFIRAVW FTLIAQSKQQ GFNAPLTLIS
KGVVPKQAGA ESFVPLLATF CMLFGRLLPT LHDVEFCDEK LLIEKPLQPT LHVRLMPFSL
AEIVQLSKTL KEVSLGLVEL AFPETRSNLN QHYRFVLGRS DSDDKKIKQQ KLIWSNLLKV
VVFVLNQIHT RDLRLGFCPE LHWTVSRLDL PLDRPADLPL TRGSRTRGIR PFQPIRDFTR
EDFENGPPMS TKQIRSITIL REIPFVVAFS KRVGILQGLV AADKMRVQGH LQAFLQGPSI
TLTVRRTHLY EDAYDKLRPE NEPDLRLKFR VQFVSSLGLD EAGIDGGGVF REFLSELIKT
AFDPNRGFFM VTTDNKLYPN PNVGDLVPDF EKHYYFIGRI LGKAIYENLL VELPLAEFFL
TKLAGKYADV DIHQLASLDP ELYKNLLYLK DYEGDVSELN LDFTVASSSF GQTQVIELKP
QGQSIPVTNS NRIEYLQLMA DYKLNVQIRQ HCIAFRKGLS NVLPVEWLYM FSNKELQILI
SGAEIPIDLE DLKKHCKYGG EYSPEHPSIV AFWSALESFD DLQKRQLLKF VTSCSRPPLL
GFKDLDPPFF IQNAGDMERL PTASTCTNLL KLPPFKTVEQ MREKLLYAIQ SGVGFELS
//
