ID A0A0L0CFG3_LUCCU Unreviewed; 1570 AA.
AC A0A0L0CFG3;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=Rap guanine nucleotide exchange factor 2 {ECO:0008006|Google:ProtNLM};
GN ORFNames=FF38_12022 {ECO:0000313|EMBL:KNC30980.1};
OS Lucilia cuprina (Green bottle fly) (Australian sheep blowfly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC Calliphoridae; Luciliinae; Lucilia.
OX NCBI_TaxID=7375 {ECO:0000313|EMBL:KNC30980.1, ECO:0000313|Proteomes:UP000037069};
RN [1] {ECO:0000313|EMBL:KNC30980.1, ECO:0000313|Proteomes:UP000037069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LS {ECO:0000313|EMBL:KNC30980.1,
RC ECO:0000313|Proteomes:UP000037069};
RC TISSUE=Full body {ECO:0000313|EMBL:KNC30980.1};
RX PubMed=26108605; DOI=10.1038/ncomms8344;
RA Anstead C.A., Korhonen P.K., Young N.D., Hall R.S., Jex A.R., Murali S.C.,
RA Hughes D.S., Lee S.F., Perry T., Stroehlein A.J., Ansell B.R.,
RA Breugelmans B., Hofmann A., Qu J., Dugan S., Lee S.L., Chao H., Dinh H.,
RA Han Y., Doddapaneni H.V., Worley K.C., Muzny D.M., Ioannidis P.,
RA Waterhouse R.M., Zdobnov E.M., James P.J., Bagnall N.H., Kotze A.C.,
RA Gibbs R.A., Richards S., Batterham P., Gasser R.B.;
RT "Lucilia cuprina genome unlocks parasitic fly biology to underpin future
RT interventions.";
RL Nat. Commun. 6:7344-7344(2015).
CC -!- SIMILARITY: Belongs to the RAPGEF2 family.
CC {ECO:0000256|ARBA:ARBA00010829}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KNC30980.1}.
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DR EMBL; JRES01000477; KNC30980.1; -; Genomic_DNA.
DR STRING; 7375.A0A0L0CFG3; -.
DR EnsemblMetazoa; KNC30980; KNC30980; FF38_12022.
DR OMA; GTCEYEF; -.
DR Proteomes; UP000037069; Unassembled WGS sequence.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 1.
DR CDD; cd00992; PDZ_signaling; 1.
DR CDD; cd01785; RA_PDZ-GEF1; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR23113; GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR23113:SF249; RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00100; cNMP; 1.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 1.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 3: Inferred from homology;
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Reference proteome {ECO:0000313|Proteomes:UP000037069}.
FT DOMAIN 113..230
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 261..393
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 396..475
FT /note="PDZ"
FT /evidence="ECO:0000259|PROSITE:PS50106"
FT DOMAIN 744..830
FT /note="Ras-associating"
FT /evidence="ECO:0000259|PROSITE:PS50200"
FT DOMAIN 855..1083
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 565..625
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 673..695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1078..1115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1150..1232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1285..1339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..25
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..614
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1078..1093
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1150..1228
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1286..1313
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1570 AA; 172667 MW; 259E3CE81A08E4B5 CRC64;
MDPYHHIRQH YQPPSRPELH QKCNRGSHSS DTSSAYSGSD TMASVYGSSL DGEEIDFSGL
VESTVDSDEE DLAESMDSLN VRDAVRDCLE KDPSERTEED VEILLEFTQG LKAFTNITLA
VRRALCSVMV FAVVDKAGTV VMSDGEELDS WSVLINGVVE IEHANGEREE LQVGDSFGIL
PTMDKLYHKG VMRTKCDDCQ FVCITQTDYY RIQHQGEENI RRHEDEEGRV VMVTELRQIG
TNDAPGTAAN NSTTAGTNAK RGHVVIRGSP ERLLQQLVEE NSMTDPFYVE DFLLTHRIFI
KNSQEVTDKL LSWFEADSNG AESVPKFASN ATAQEIRDRV TRVVLLWVNN HFTDFEADHE
MMEFLEIFEA GLERKKLLSQ LRLLHIACAA KARMRSCILA RSSRDESLNF KIIGGYEMRG
ACVATGGCGI YISHVERASK AQEAGLKRGD QIHEVNGQSF EHVTSKRALE ILMGSTHLSI
TVKSNLLGFK EMMQAVEITA NGGINSANGN VAGLGSKSLR SPRRICANDV AKLHGRADST
TDELTAAAAN RTHIVRLSSV DMLLHSSPGE DQTDRGVQSP GTPIQTPSSG NMASNFMNNL
LQSVNASGKK DSNGMQGDSK GGGFMTLAPK RRLKIALAKM NLLHKNVTGL QASNLNDSSD
ALLNVGCNEK QKSGSLSSAS SSSHGPGRLY QSQSNPDLTS LQYEDASAPN NNVLNICNPS
NYLSASVHRP SAVSTTGSLV PDYPEHVLKV FKADQTCKYL LINKETTAHE VVMLALQEFG
IHDPSSNFSL CEVSVGEGGM VKQRRLPDQL QNLAERIGFA SRYYLKTNGI SETLVPDELA
LELVRESNVH FLQLNAYELA IQLTLQDFSI FRQIESTEYV DDLFNLKTKY GVPMLSKFSE
LVNREMFWVV TEICGEHNIV RRMKIVKQFI KIARHCKECR NFNSMFAIIS GLGHAAVSRL
RQTWEKLPSK YQRLFSDLQD LMDPSRNMSK YRQLVSSELL NQHPILPFYP IVRKDLTFIH
LGNDTMVEDL INFEKLRMIA KEVRLLSHMC SSPYDLLAIL ELKGQSPSNA MFSLNQMSTS
QGGSNQGPTI IAANAGNATI KRRKKSTAAP NPKKMFEEAQ MVRRVKAYLN NLKIINDEDI
LHKFSLECEP AHTSSNSTSS QHGGNSSRTG GDQLSIYSHT SNSSSAPNSS LSLRRRHPSS
PTLSTTSSTS STSDNRRNNM NPNCPKFGTA SPQAVRKILS LSESTKIRPH QPFPRPAATL
PGNIPLLAQP HHLHSHGFNP AASAALAAAS TPTPSPCSHR RLASGSNSMP ASMIPPPRSI
HERSHSDTPA PPPPLPSVDL NLESSSVTTF RDLPLRKSVT SDEDYQLIQI DCTNLRFFEF
LPKCVRIGSV SSNDSGLCSY AQHDTSSSSS VYTAADCRLL QQISNTAATR NINTQCSQRA
LSPHTLHPAT ATTTVTQTAT SMNALPLPQT QSQQQLQLPP PTLTPPPYLM RGQNLQMFMT
NGGTNAVNGG THAPPPQLLH NRPLSPPLQQ PNMPPPYATA AAMLHHNHRH SNMHGAAGCP
MCPMPPPMNQ
//
