ID A0A0L0DCI4_THETB Unreviewed; 940 AA.
AC A0A0L0DCI4;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=AMSG_06084 {ECO:0000313|EMBL:KNC49806.1};
OS Thecamonas trahens ATCC 50062.
OC Eukaryota; Apusozoa; Apusomonadida; Apusomonadidae; Thecamonas.
OX NCBI_TaxID=461836 {ECO:0000313|EMBL:KNC49806.1, ECO:0000313|Proteomes:UP000054408};
RN [1] {ECO:0000313|EMBL:KNC49806.1, ECO:0000313|Proteomes:UP000054408}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50062 {ECO:0000313|EMBL:KNC49806.1,
RC ECO:0000313|Proteomes:UP000054408};
RG The Broad Institute Genome Sequencing Platform;
RA Russ C., Cuomo C., Shea T., Young S.K., Zeng Q., Koehrsen M., Haas B.,
RA Borodovsky M., Guigo R., Alvarado L., Berlin A., Bochicchio J.,
RA Borenstein D., Chapman S., Chen Z., Freedman E., Gellesch M., Goldberg J.,
RA Griggs A., Gujja S., Heilman E., Heiman D., Hepburn T., Howarth C., Jen D.,
RA Larson L., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., Thomson T., Walk T., White J., Yandava C.,
RA Burger G., Gray M.W., Holland P.W.H., King N., Lang F.B.F., Roger A.J.,
RA Ruiz-Trillo I., Lander E., Nusbaum C.;
RT "The Genome Sequence of Thecamonas trahens ATCC 50062.";
RL Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00104}.
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DR EMBL; GL349457; KNC49806.1; -; Genomic_DNA.
DR RefSeq; XP_013757590.1; XM_013902136.1.
DR AlphaFoldDB; A0A0L0DCI4; -.
DR STRING; 461836.A0A0L0DCI4; -.
DR EnsemblProtists; KNC49806; KNC49806; AMSG_06084.
DR GeneID; 25565344; -.
DR eggNOG; KOG0939; Eukaryota.
DR OrthoDB; 1122263at2759; -.
DR Proteomes; UP000054408; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR017868; Filamin/ABP280_repeat-like.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50194; FILAMIN_REPEAT; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000054408};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT TRANSMEM 12..36
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REPEAT 207..244
FT /note="Filamin"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00087"
FT DOMAIN 649..940
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 136..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 371..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 940 AA; 98066 MW; 038CEFCCE60CAA53 CRC64;
MWDERFESGE VLGIGFVWVC LVVPTVAVLY LLGWLLSTAG GLLATRLGAT ACVGLGTLGL
WISWRRAAAR DTHVSSSMVM VSLAAHGSLS ATNSRLVAPV DVLRPGEPTA IVVKLATADG
AGVVAKDVGG LGAGANGAAA GRGPYATAEE GAGGDGEGEA GIEGVVPRHV AALGLGIAVQ
RAASAVESGG PHVGGKGGGV LESVALREDG AVAVVLSPRV AGMYRLVATL HGVPVTGSPL
ELEVAPGTID VRGCVVGGDG LGPAPLVVFR EARVTIEARD GYHNRIFAAA PYEARLDDYH
NGLYALVFTV HAEGVFTGRL LLDGESLATW NVVVLSVTQA AALREALTPP LTARSAISAL
SASLKHGLTM SEPATPSEAS VGKVGGSSFD SGESGMVGRT LSATSLVGLE AEASSGGSGG
VVSKVAFKVV LHSASLTTMV VDERLAVMGS SGDEVVVEES EVLKLVITPR RLLLKRYVLL
GLVPQRVAKM RLGRGARLVL DPGSQTEFTI SNSRESLRMA VVRHEEREVL YGAFVALQAA
AEERGGREHA AAALEAKTAA LYAELQAQHA RAARESGVVK WVNLSLQVEG DASESLPETL
AGLWPAAVRE LGGLRSSKWR KRFRLRGASS FVGWAAALAS HALGSGALGL FERTGRTAHP
QAGRTSGSKS GLAALRLCGR LAAKIVYDGA VHGASEVGRR ELGVPLSLSL RRLVVGLPVT
AECLAVDAPM VYAEQVLPLA FNDASASSAM AFTIRDGETE VELVPDGASH AVTERNKEWY
LALLAHYWAV TRVEKQVMAF LEGFYELVPA ELVIGIDECE LEVLLHGLPR VEVRRLQSAA
VYAAPERLSS GSEVAAWVWD AVDAFAPLDQ ARFLHFVLGT CVIDDSADPG SVAVVLAPAD
GSVFAARRVV RTLDVPVDML SSYHDVYDAL LAAIYDDRDS
//
