ID A0A0L0DRM9_THETB Unreviewed; 979 AA.
AC A0A0L0DRM9;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=AMSG_10236 {ECO:0000313|EMBL:KNC54989.1};
OS Thecamonas trahens ATCC 50062.
OC Eukaryota; Apusozoa; Apusomonadida; Apusomonadidae; Thecamonas.
OX NCBI_TaxID=461836 {ECO:0000313|EMBL:KNC54989.1, ECO:0000313|Proteomes:UP000054408};
RN [1] {ECO:0000313|EMBL:KNC54989.1, ECO:0000313|Proteomes:UP000054408}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50062 {ECO:0000313|EMBL:KNC54989.1,
RC ECO:0000313|Proteomes:UP000054408};
RG The Broad Institute Genome Sequencing Platform;
RA Russ C., Cuomo C., Shea T., Young S.K., Zeng Q., Koehrsen M., Haas B.,
RA Borodovsky M., Guigo R., Alvarado L., Berlin A., Bochicchio J.,
RA Borenstein D., Chapman S., Chen Z., Freedman E., Gellesch M., Goldberg J.,
RA Griggs A., Gujja S., Heilman E., Heiman D., Hepburn T., Howarth C., Jen D.,
RA Larson L., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., Thomson T., Walk T., White J., Yandava C.,
RA Burger G., Gray M.W., Holland P.W.H., King N., Lang F.B.F., Roger A.J.,
RA Ruiz-Trillo I., Lander E., Nusbaum C.;
RT "The Genome Sequence of Thecamonas trahens ATCC 50062.";
RL Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; GL349493; KNC54989.1; -; Genomic_DNA.
DR RefSeq; XP_013753434.1; XM_013897980.1.
DR AlphaFoldDB; A0A0L0DRM9; -.
DR STRING; 461836.A0A0L0DRM9; -.
DR EnsemblProtists; KNC54989; KNC54989; AMSG_10236.
DR GeneID; 25568510; -.
DR eggNOG; KOG0433; Eukaryota.
DR OMA; HCWRCKT; -.
DR OrthoDB; 656at2759; -.
DR Proteomes; UP000054408; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000054408}.
FT DOMAIN 21..107
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 142..692
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 739..896
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT COILED 89..135
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 979 AA; 104140 MW; 73D7303FE574C7C2 CRC64;
MRADAAVREK EKMETTTTQL YQWSHDQRTA GARPPFVLHD GPPFANGRLH MGHALNKILK
DVINRHALLS GSAVHYVPGW DTHGLPIELK ALDAARKDSA KAIKKIKKEL KRAIKAEAGD
EVARLEAQLA AAQSAAAGRE PTKIRKLAAE FAASAATEQK DALLRWGVMG DYDNAYYTMA
PAYEAAQLEL WSSLYHRGLF SRRLKPVYWS PSTQTALAEA ELEYKPHTST AVHVAFRARA
SDQLLAALGV DLAPRPIDAV AWTTTPWTLV ANQGLAFGPA LDYALVESGA AADAPLVLVA
SARIGALVDA GVLPSSPRVV ADGLSAEAIA GTVFSNPVVA ERDSVALMGD HVTDDAGTGV
VHTAPGHGAE DYTAMARAGL LNTDDPLFCP VDGAGKYTDA LASVGLGQLV GQPVLDAGTK
SVVELLHAAE NAPLLAAAPL EHSYPYDWRT AKPIIFRATM QWFMDLSAVK GAALNALDDV
TMVPPEGAAR LAGMIGTRDE WCISRQRVWG VPIPVFYHRE TGEVLATRES LSHVVELVAR
HGTNAWFDRP VSELLPASLA DEAHKYVKSV DTMDVWFDSG SSWYAVLAKS LGSPVQADVY
LEGSDQHRGW FQSSLITSSA LQDPPRAPYK TLITNGFVLD GAGNKMAKSL GNVVDPDQVV
HGGANAKKEP PYGADVLRLW VAAQEYTRDV RLGPQGLSSA ADTYRKLRNT AKFVLGNIAD
APAELLMAAG APAAASPLDR LVLSSLHELN TKVQAAYSSY AFHAGLFALL NYVNTFLSAF
YLDSAKDTLY AEAADDSARR AVQYVLYRAL DVVTRAAAPI APFLAEEVWG HMPLDTRRDD
HFSLFQSGYA ETGPLRDPAS DALRDALLDL RGEANKALEG LRQAKAIGGS LEAQVVVAAD
VLPESLAAWL ATLSPGDLAN VFGVSSAQLE SSAATGGATV LAAPASGSKC DRCWKVTPDV
SGGGLCGRCA AVVGGEAAM
//
