ID A0A0L0FX09_9EUKA Unreviewed; 5930 AA.
AC A0A0L0FX09;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=Midasin {ECO:0000256|ARBA:ARBA00017143};
GN ORFNames=SARC_06594 {ECO:0000313|EMBL:KNC81071.1};
OS Sphaeroforma arctica JP610.
OC Eukaryota; Ichthyosporea; Ichthyophonida; Sphaeroforma.
OX NCBI_TaxID=667725 {ECO:0000313|EMBL:KNC81071.1, ECO:0000313|Proteomes:UP000054560};
RN [1] {ECO:0000313|EMBL:KNC81071.1, ECO:0000313|Proteomes:UP000054560}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JP610 {ECO:0000313|EMBL:KNC81071.1,
RC ECO:0000313|Proteomes:UP000054560};
RG The Broad Institute Genome Sequencing Platform;
RA Russ C., Cuomo C., Young S.K., Zeng Q., Gargeya S., Alvarado L., Berlin A.,
RA Chapman S.B., Chen Z., Freedman E., Gellesch M., Goldberg J., Griggs A.,
RA Gujja S., Heilman E., Heiman D., Howarth C., Mehta T., Neiman D.,
RA Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C.,
RA Sykes S., White J., Yandava C., Burger G., Gray M.W., Holland P.W.H.,
RA King N., Lang F.B.F., Roger A.J., Ruiz-Trillo I., Haas B., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Sphaeroforma arctica JP610.";
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}. Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|ARBA:ARBA00007188}.
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DR EMBL; KQ242072; KNC81071.1; -; Genomic_DNA.
DR RefSeq; XP_014154973.1; XM_014299498.1.
DR STRING; 667725.A0A0L0FX09; -.
DR EnsemblProtists; KNC81071; KNC81071; SARC_06594.
DR GeneID; 25907098; -.
DR eggNOG; KOG1808; Eukaryota.
DR OrthoDB; 1221059at2759; -.
DR Proteomes; UP000054560; Unassembled WGS sequence.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 6.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR048617; MDN1_AAA_lid_4.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 8.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR Pfam; PF21108; MDN1_4th; 1.
DR PIRSF; PIRSF010340; Midasin; 1.
DR SMART; SM00382; AAA; 5.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000054560};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 27..5930
FT /note="Midasin"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5005538951"
FT DOMAIN 5728..5919
FT /note="VWFA"
FT /evidence="ECO:0000259|PROSITE:PS50234"
FT REGION 215..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 498..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 803..826
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3922..3941
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4994..5050
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5065..5618
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 498..525
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 526..540
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5066..5116
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5117..5161
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5184..5200
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5201..5233
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5234..5254
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5256..5273
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5302..5318
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5319..5342
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5362..5377
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5390..5406
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5456..5472
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5473..5491
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5529..5567
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5568..5589
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5598..5615
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5930 AA; 655836 MW; F8895B3BC4B241DE CRC64;
MCHWTHQSKK SLLLVVSELL LDPALTSTVA RVFRPVLVDL IIKLIEPNEE QHGAHSIPTP
FSLDRHDSIG ACLSVLLMSA PQLQSQVLSY YEYNPPPFER ISRSHSPSQT TQSESYEDMA
ATCYRLLRFC PALRTRWNWG PLYILLTNEN PTTRVYVAKS IALLLKLDNQ QTTRLFEVYL
DPSADLQLTL DQTQNDTRAI ESYLLSLKDT STAASTDSAE LPTTDSTPTT AAKSSDESDV
LGASDLSGTL VDVCGVLLPR TREVLQPITS GAPLDGARAS ENSAATAYVM TDTTSGNLRA
LALALSQGLP ILLEGVAGAG KSSLVNELAR CTGKDTTKGV LKVHLGDQTD SKTLLGTYVC
TDIPGEFRWQ PGVLTQAMRS GKWLIVEDID LAPIDVLSVL VPVLEKREVF IPGRGEVVRA
ARGFQLFATQ TLSESGTARK GGGRGSTMLA NLWTRVVVQA LTLTEMRRVI DTIYPALAPF
SSLMLEVYQT LANIGRRTKD PEEVKEATTE IDGESDTHDS DGDTAMNDSL DSQQQREQDA
GGYAPSEMAA ISRIRRAYTY RDLLKWCTRC CQYAAYHTTI TSVSQNLLTP HLFYEAADVL
AGMFPMVVRR RLLHLIGHKL GISTESVEHR GTTYCPEVSW SDDTVVVGRI TLPKGASGSG
MRKESSDSGS YALTRLHARL IESVAACVSK GESVLLVGET GTGKTTLVQR LALSMNQKLV
VVNMSQQSDL SDLVGGFRPV DVRVLATPLV DTFDRVFKAT FSQKANAKFL NEVRSAYARG
KWKRFLALLN AAGKMYTKKF GPSAAGDQSE SRANGNAAAP VQKAARSDTQ GLQLEWEGLM
EDVQKFTAQM KHVDHGFAFS FVEGTLVKAM RSGHWILLDE MNLAAAETLE CLNGLLESPT
GSLVLTERGD QTPIPRHPQF RLFGCMNPAT DVGKRNLPPG VRSRFTELYV DELTHRADLV
ILANQYLSRL GNLPTTVVDA VVSLYLDAIR YAESELTDGA NQKPHYSLRT LCRALDGSRL
LVHRYGLQRA LYEGFCSAFL TSLGRESYLK MQSLVHKYVG FGMSPKQVNR ILPNPALLDA
SGNVSETALA SASGGDDRSP IDEGLGWCRV EHIWLKCGDT APIVAQEDTK FVLTESTREN
LRRIARALVS GQFPVLLQGP TSCGKTSMVE YMAQRSGHRF VRINNHEHTD LQEYLGAYTA
DDKGRLVFQE GVLVQAVRHG YWVVLDELNL APTDVLEALN RLLDDNRELM IPETQEVIKP
HPHFRLFATQ NPPGVYGGRK VLSRAFRNRF VELHFDDIPE NELETILAKR TQIAPSYCTK
LVAILKDLQK RRQGTRLFAG KHGFITLRDL FRWAGRDANG YDQLAEDGYM VLAEKCRRDE
EKQIVKETIE KHLKVSLDPT AMYGASHEGF AAMQTMIKQA SADPNNSLYP FRNIVATQSM
LRLITLVQRC LQFKEPVLLV GETGCGKTTV FQLLTAIQGQ LLRTVNCHQH TETADFIGGL
RPVRGRDGRI AAVRIDAHSY VSDVLSDESL GCTVKLPEES EWAASTNEAL RAIVSTVVQS
IVDGKYSSTA DLRTPLVARG RELLAEFNAA TTLFAWHDGP LVQAMQCGDM FMIDEISLAD
DSVLERLNSV LEPYRGLTLA EKGSDHVQEI VGVEDFRIVA TMNPGGDFGK KELSPALRNR
FTEIWVPSVS DPADLKAILD ASMSSYEGLS AFSEPILSFV NWFRVNHNPK VVVSLRNILG
WSNFMVNCVH NRNSTGGDAF VHGGCMAFVD ALGTGSSVAY GSHTRDTKKA CVAHLTKLAA
IAGGAEATSD APVSERRIGA PVVRESATVG SEGQFEFCVD SFAIPIGPLY SVERSSLTTS
FALSAPTTLS NMQRVLRGLQ LTMPILLEGS PGVGKTSLVT AVAKAAGYPL VRINLSDQTD
IMDLFGSDLP VEGGQGGEFA WRDGPFLEAM KNGKWILLDE LNLASQSVLE GLNACLDHRG
VVYIPELDRS FECKAPLRVF ACQNPLAQGG GRKGLPKSFL NRFTRVYVEE LDATDLRTIA
KSMFPHIDQD IIDSMISFNS RLHHATMVDR AFGSKGGPWE FNLRDVLRWC EVMSNNQSQG
RYSPNDFLDF LYLQRMRTES DRTAVLDLYH QCFAHDKLAF QHRIDEYPSI RLTESHVQVG
STLAKRHHAS TADLSVGARE LLATHALRRP LKHVLTASEM RWMSIIVGDS AAGKTSLIRL
AASLTGNRLH EFAMNSAVDT SELLGGYEQL DMLRRRTEIV TRASALVAMV SQALVLVPGV
ASAQSGASAQ QALSMVQQVH NTWQMYQLTD QHTPSPTAPD GEASDAVGFS AEQRDAMFAF
LDQIEAAVRS CAETPATVGI TTTCSKLRQA VDALATLDAT HVRGSFEWVD GILVRALKEG
HWLLIDNANL CSPSVMDRLN PLMEPNGTLV LTERGVNTHG ALECIIPHHN FRLILSVDPK
YGELSRAMRN RGVEISLVNA DAPASSRNLA SLPTTPTHPT DMVILLQTAG VANPVVTSLL
VACHYAIRRS VPASADFPLS VRDVLHAGRL ISEMLHRGFD IQRALRTALV EVYVRCRRVA
SVRATIQEVI ESHLSSFSPT ETHTLTDTDT TAARQPLPAA ELWYQPALFS CDTLVRAPRM
AIIARDGVYL THLKNLYARE RVSALLAASS VDAAGLEQFG TASSSAWSYL PADVCVAKAM
CTAVVSRGCG IDATLKVVTD TEFLTTLKDQ LMWAGAYFLE RTCLDDVERR VTWVNEWTKT
PESVVGREMH AVSKGLQRGL YLLASCSHLE TLRKAREQLS ACLALPTGLL DSQPIACKAN
IQLWTALHSS LGNTTTNDET QNQLNTAWNV QCRVGNVLSV LYCRRWRVLF QEDQLQARTR
RTSHREYSIL QQSFLIYQKT LPSSKALHPL CTRFFGFIVA LDRWLSDILA SGFVTAVDTS
KAHSVSVVDS DITSLAHLLS ARDALYAAAN TSFSREKQTE IVSSLVVHWR WFLRALCQFR
EHSTVTVAGE SSSTASDYAD ALNAACEYVS DCLQLRTAVN GLVLWKNWLR PRGYKTLREY
DVMRKLLDLS SDLDVYKPTA VSAMFPAFPV WLLSLPGHPF LEKANRTTIQ TLTEGIATML
SVEQAHAVGE TQTNGAANAV ARSGAANRQA MLEKIYQVLE QIALPVSASA NTELLSGEKQ
QSGSELSTVE LWPVYDHQLL MEEQRLVSQI SALVVLLRTE AHTSQPKLSA QVRAVLLQRI
EAHMSRNRMD SYSRPASDWA PWLCLRWLLS LGEDRLPWES VVPVLTNAIA SFHRGVRSHL
LAEVAYMPLA AKLVESAANV GEQFGTEDAL DAMTQVLQQG EQKLAKTSRL PASSAATQLG
LNTDVGYMTA LVAQFSSCSI GDMPRRITQL QKVEHVVAHR AQALTATHLQ TVLDNGEPVE
GSVETKTRDL QHFLALESTL FGVLLGFRKG FSHEDYLRLC QLAHSAIASR VDCAEDTAPA
HTAADVHPFS SEDSEALAAI VERCANASLR ECAVWCIRPF LQLYAEQAEP QRVLAMGWVY
MGLLQIHLGA CASVVDPSTS YSLKERDGLL RQRAIEARMK VVSTTEATLD GGDGSGGVDM
KECVAEMNHV HEKLKHTSMR VLVRPGDDTF SDLRAEVSRY LSSIGSARVV LDLCACIVAL
LREMRDGAAA QKDTHAASVS VSDVLSRWRM WRESQRSFCS SLRRFSGMFV DIVDPLMLAI
DEMVASVDTL GDQLVHQMDM LYSAPSPVNA TPITGDLQAY MCTQIRPRMT RLVTELLRFP
SFQKGSGCAP FRYVHRTQDL TLAAGHVQAN SPILSLITQF SGVPLAMSSL RHAYVHVLDT
QTVGAAHVTD LGRMFSDVVD VWTAYEEQKL AKERADALAF KTKEKVFVLE DERTLEERLY
QQQCPSFDDE FTDITKDYHT NASATASKAK PSEDSATLTN PLSPEATLHI TDMHAAIFGT
LGNHASMSHA DKHTIEQEHL AAFRYNYEAT LPLLEGLGLE LSFDSDDRAQ MDTLVAISLR
AKMTRKHMLQ TQHNNTLSVQ SKEWSEAAPA ADIEVLSALL NPGAQPILQP SPLRINHYTD
TSLAEARRLG EIAVPMRVDA TTHLVTWPEH PVLMGIVAVI DRILSFSIQA SPLMKLLSAA
ELLLRQGMEW EAFAASHVSL KVHTEAVTRF ILDMRKKELD QWSLALDSTH QEHEALSRRL
WFHLYSVINA DPTLESELGR EDAIESHHKE IFTVVEEMVQ TSTMGNFACR LRLIAQFHMQ
LKAELKSGIA GYSISAKVKL HDMLGSFYAY YRQYLPGIQA KLHGAKKPLE RQLREHVKLA
KWNDINYWAM KQAGDKTHRA LHKFTTKWST VLEMPLRTYL TEIEAATIEY PPAQPSAAMG
SIITRVCHRD NWKPSTSVQA IGALSSPVLS RYEEWFHVSD PTRFHSRLAA LTGRLTKVCD
KQIMPALNPS TILGIEEFAV HIIQRVEQYR LWDAQDIKDT EATLARKKEQ ADAGADEEKP
ADKDSRALHK VMKRKDLAEL FRQLSAAGLS YRSVAAEDES ALLSHLFTEA HLEDDQLCPQ
TSDVADLAAK INDYFYKCVS RMVIIRKLYF GGRAQDLTPQ DADRARGYSE HFLRLVVAQR
RFLGDVSEEL SNFKRFLKHT VHLGNRLVTT NGSGPVVATH ALVLECWNET MDTVNTMARV
VRETTAAISL HEESTRTEST IRNRLSSALA ELHRVRTALH ALKHEHIGVF ESKRIDTAPP
PMVVEVGIVT AGLDDVYARV GKVSEGLSGF LAGVVDSPNW PEQRSLLNSL RDVCAATHAA
ESRYRTSLRP HFSAELTPPV ADMGAVPETV RERAYAFERD FDALIANVLI RVQRIVKLKE
EHEQDTPEST AEWEDGFDPS KFTPNYLTRS HTYMTSVLKA AKLPAMNAAF ASLSAQIAAM
GDDVDAEYNA DLLTACAGML VRAVPVLERY GVILSQAMDE FVCLHKSVSK MYYILLNLFS
DLLAKGFCTP PDLQDATADE QTGGEMAGMG EGQGENDVSE QIENEDQLTG TKDEEQNDEG
GNVPEEDNAI EMEQEFDGDL YDMDKKELED MEDDKDDGEE KEEQDMDDEM GDTNMDDADV
VDERMWGDDE EDNEISEKEK KEDGKSMTNE NEESEIVAKQ DGDDEKPKDD KKKEEQENAE
KPQPEPLPSQ EEDQNNEDLV NEVQELEEDH HHHPQEQPND DQAADDENPD VDLPEDMQLD
DDAGDDKDEH DGPDGDGEET APMEDEKAMA EEYDGAQELN DPEEGDDADP EGDVATDTEQ
KGNDEEEEED GKQGEDSLQA AGESDQGDDE EEGDEGQPPK LMEDDNDVEI GAEQGIKEDH
GEEGEGADEE EEEKHKPESS KMDTTSVGVN AEGGQRNSGE TEEMETEDGP SSATDATSGQ
SKSGTDDKQA SLERGNDGEN DSGSKPQAMP EVNPFRSLGS TLQKWKEKLN VMDVEEEDPN
AEDGANDGDN DEEMGGEGMR QFMKDEDEGQ ADDQVLGVAT EEQLQQNQGM PDEPEGEKVE
EETEMDVDET EGEKKPEDSE ESGQKESSKS QAKSARNADK AEAKDSQADV EEEEIDSVDA
EDATEEDKSD DKDANDGPDS SIYTQKNSTD ASQDADDLAL PTEDEIVEKR EAVEKILEAW
QDRAHDPNAD AVGERLWQEY EAITSGLSQD LCEQLRVILE PTLATRLQGD YRTGKRLNLK
RVIPYIASHF KKDKIWLRRT RPAKREYQVL LTIDDSESMS DSHSASLAYE AVTMISKALT
TLEVGQLSVM SFGDQVKLLH PFHEQFGDGT GGSILQQLSF NQKNTNFEQL LETSYSHLIS
SVVSTDLPVS QLQLVISDGV CPDVESVRKW IRAYSDAGIL LVFVVLDNPK AKFSILDYKT
VTYPNGALTL TPYLETFPFP YYVVLRNINT LPDILADALR QWFELLRDDS
//
