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Database: UniProt
Entry: A0A0L0NAU6_9HYPO
LinkDB: A0A0L0NAU6_9HYPO
Original site: A0A0L0NAU6_9HYPO 
ID   A0A0L0NAU6_9HYPO        Unreviewed;       642 AA.
AC   A0A0L0NAU6;
DT   11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT   11-NOV-2015, sequence version 1.
DT   03-JUL-2019, entry version 21.
DE   RecName: Full=Urease {ECO:0000256|RuleBase:RU000510};
DE            EC=3.5.1.5 {ECO:0000256|RuleBase:RU000510};
GN   ORFNames=TOPH_03921 {ECO:0000313|EMBL:KND91166.1};
OS   Tolypocladium ophioglossoides CBS 100239.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Sordariomycetes; Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae;
OC   Tolypocladium.
OX   NCBI_TaxID=1163406 {ECO:0000313|EMBL:KND91166.1, ECO:0000313|Proteomes:UP000036947};
RN   [1] {ECO:0000313|EMBL:KND91166.1, ECO:0000313|Proteomes:UP000036947}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 100239 {ECO:0000313|EMBL:KND91166.1,
RC   ECO:0000313|Proteomes:UP000036947};
RX   PubMed=26215153; DOI=10.1186/s12864-015-1777-9;
RA   Quandt C.A., Bushley K.E., Spatafora J.W.;
RT   "The genome of the truffle-parasite Tolypocladium ophioglossoides and
RT   the evolution of antifungal peptaibiotics.";
RL   BMC Genomics 16:553-553(2015).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|RuleBase:RU000510};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRSR:PIRSR611612-51,
CC         ECO:0000256|RuleBase:RU000510};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRSR:PIRSR611612-51, ECO:0000256|RuleBase:RU000510};
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR611612-50}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases
CC       superfamily. Urease alpha subunit family.
CC       {ECO:0000256|RuleBase:RU004158}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:KND91166.1}.
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DR   EMBL; LFRF01000009; KND91166.1; -; Genomic_DNA.
DR   EnsemblFungi; KND91166; KND91166; TOPH_03921.
DR   OrthoDB; 183108at2759; -.
DR   Proteomes; UP000036947; Unassembled WGS sequence.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:InterPro.
DR   CDD; cd00375; Urease_alpha; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51338; SSF51338; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000036947};
KW   Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00700,
KW   ECO:0000256|RuleBase:RU000510};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR611612-51,
KW   ECO:0000256|RuleBase:RU000510};
KW   Nickel {ECO:0000256|PIRSR:PIRSR611612-51,
KW   ECO:0000256|RuleBase:RU000510};
KW   Reference proteome {ECO:0000313|Proteomes:UP000036947}.
FT   DOMAIN      233    642       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    424    424       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       238    238       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       240    240       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       321    321       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       321    321       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       350    350       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       376    376       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       464    464       Nickel 1. {ECO:0000256|PIRSR:PIRSR611612-
FT                                51}.
FT   BINDING     323    323       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     321    321       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR611612-50}.
SQ   SEQUENCE   642 AA;  68045 MW;  4AA1D0C223E7876C CRC64;
     MALGATMLGR RHVLPAVCST LREIQVEGTF PMGTYLVTVH NPIATDDGHL RRALYGSFLP
     VPDAEAFPLP PDAEYALARQ PGAVVAPAPE PAPEPAPPAL DAFTMDRAAC ATMFGPTTGD
     RVRLGNTDLW VRVERDMTTY GDECKFGGGK TLREGMGQAT GRADADSLDV VVTNALIVDW
     TGIYKADIGI KAGLIVGIGK AGSPDVMDGV TPXMVVGSCT DVVAGEGKII TAGGIDTHVH
     FICPQHAQES LASGITTMLG GGTGPSAGSN ATTCTPGKHL LRSMLQACDT LPVNVGITGK
     GCDSSPVALR EQILAGACGL KIHEDWGATP AAIDACLTAC DELDVQCCIH TDTLNESGFV
     ESTIAAIKGR TIHTYHTEGA GGGHAPDIIS VVEHPNVLPA STNPTRPYTR NTLDEHLDML
     MVCHHLSKNI PEDVAFAESR IRAETIAAED VLQDMGAISM MSSDSQAMGR CGEVILRTWN
     TAHKNKVQRG LLKEDEGTGA DNFRVRRYVS KYTINPAVAQ GFSHLVGSVE VGKLADLVIW
     DPAWFGTKPS FVLKGGLIAW AQMVLFVSAA CVSSGTAAGY GLRSRVEPVR NCRGLTKKDM
     RLNDATPRMR VDPESYTVEA DGEVCTAEPA ETLPLSQAWY VF
//
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