ID   A0A0L1KZR7_9EUGL        Unreviewed;       715 AA.
AC   A0A0L1KZR7;
DT   11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT   11-NOV-2015, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   SubName: Full=Methyltransferase {ECO:0000313|EMBL:KNH09565.1};
GN   ORFNames=XU18_0250 {ECO:0000313|EMBL:KNH09565.1};
OS   Perkinsela sp. CCAP 1560/4.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Prokinetoplastina;
OC   Ichthyobodonidae; Perkinsela.
OX   NCBI_TaxID=1314962 {ECO:0000313|EMBL:KNH09565.1, ECO:0000313|Proteomes:UP000036983};
RN   [1] {ECO:0000313|EMBL:KNH09565.1, ECO:0000313|Proteomes:UP000036983}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCAP 1560/4 {ECO:0000313|EMBL:KNH09565.1};
RA   Tanifuji G., David V., Flegontov P., Gerasimov E., Hashimi H.,
RA   Logacheva M., Maruyama S., Onodera N., Gray M., Archibald J., Curtis B.,
RA   Cenci U., Lukes J.;
RT   "Draft genomes of Paramoeba pemaquidensis and its kinetoplastid
RT   endosymbiont Perkinsela sp. CCAP 1560/4.";
RL   Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KNH09565.1}.
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DR   EMBL; LFNC01000001; KNH09565.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0L1KZR7; -.
DR   EnsemblProtists; KNH09565; KNH09565; XU18_0250.
DR   OrthoDB; 126975at2759; -.
DR   Proteomes; UP000036983; Unassembled WGS sequence.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR   InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR   InterPro; IPR023267; RCMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR22808; NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR   PANTHER; PTHR22808:SF10; PUTATIVE-RELATED; 1.
DR   Pfam; PF01189; Methyltr_RsmB-F; 1.
DR   PRINTS; PR02008; RCMTFAMILY.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000036983};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW   ProRule:PRU01023};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01023};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}.
FT   DOMAIN          68..413
FT                   /note="SAM-dependent MTase RsmB/NOP-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51686"
FT   ACT_SITE        307
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         174..180
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         202
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         230
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         254
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ   SEQUENCE   715 AA;  79747 MW;  5CB93D5F65DC9627 CRC64;
     METEIPSAKA IKYAEDREKR IARRRLLDVE LNERFLSYYR STLFASDAQT TAESSEAIDC
     NAGSWEKFTE ILRVPLHVVF RVEETHPLST EVMGLLADIQ VAAPCQILPS NLAYQCSNAN
     FDFPRLKQVF QLLNASALIT FQESVSMIPS IALIRSLKQN AQKEGSGPYI LDMCAAPGSK
     SLHFLDALYS QFDSRGVLVS VEKDPVKATQ TLPARLKRAQ SPCCLAVQGD ATRFPSLYCP
     TEGKQILFDG VLCDVPCSGD GTSRKDNTIQ KRWDENFAVK LHPKQRAIFH RGLELVNSNG
     YCVYSTCSLN PIENECVFTR TIDEFGSSFV TVENIPSVVP EFRFSPAISL AEIHGSHQVF
     ETLENQINRV NGESAFTFAK AADTLRINPD YLGRALPHVN NSGGFFTTLL KRTAVSPATT
     DMVPSDTISH HAYPNSFSHW GAHKNFRDLN EKEEKFLAEF YGIDAGKLEE SYGLRLIVHG
     VNDEKPKRIL LLSKECYSLL HCLLRKAKKE RITHVGVRVF SFWQSKLFSG DYHIPFRTTY
     EGARHLGALS NGSRTLHLCV MKHLSLVHLL LQKGIAESST LTEVYQESNG SPIALIGDCI
     SSLEPKCFNP YILSVFERHC LKDKHIEGAP DCGNGMEGHI APGPLWIAIH LSCLRNAQGS
     KRNNLGDFGK KDVFGPKSIL WLSGVLYFTR LEITTKEPIR KGISIVLHQS LSGLL
//