ID A0A0L6Z6N0_9CLOT Unreviewed; 775 AA.
AC A0A0L6Z6N0;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE SubName: Full=Putative 3-hydroxyacyl-CoA dehydrogenase {ECO:0000313|EMBL:KOA18615.1};
DE EC=1.1.1.35 {ECO:0000313|EMBL:KOA18615.1};
GN Name=fadN {ECO:0000313|EMBL:KOA18615.1};
GN ORFNames=CLHOM_28990 {ECO:0000313|EMBL:KOA18615.1};
OS Clostridium homopropionicum DSM 5847.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1121318 {ECO:0000313|EMBL:KOA18615.1, ECO:0000313|Proteomes:UP000037043};
RN [1] {ECO:0000313|Proteomes:UP000037043}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5847 {ECO:0000313|Proteomes:UP000037043};
RA Poehlein A., Beck M., Schiel-Bengelsdorf B., Bengelsdorf F.R., Daniel R.,
RA Duerre P.;
RT "Genome sequence of the strict anaerobe Clostridium homopropionicum LuHBu1
RT (DSM 5847T).";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOA18615.1}.
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DR EMBL; LHUR01000036; KOA18615.1; -; Genomic_DNA.
DR RefSeq; WP_052222369.1; NZ_LHUR01000036.1.
DR AlphaFoldDB; A0A0L6Z6N0; -.
DR STRING; 36844.SAMN04488501_11036; -.
DR PATRIC; fig|1121318.3.peg.2909; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000037043; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR48075; 3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN; 1.
DR PANTHER; PTHR48075:SF7; 3-HYDROXYACYL-COA DEHYDROGENASE-RELATED; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 4: Predicted;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000313|EMBL:KOA18615.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000037043}.
FT DOMAIN 3..200
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 204..303
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 775 AA; 86714 MW; 02BC3A67318BCDA5 CRC64;
MKKVCVLGAG TMGAGIAAHI VGAGIPVCLL DIAPKKLTEK EKAKGLTLDS FGVRNRFAIA
GKERVTDPKN KSIYDKTLGD MIEVGNFADN MDMLTECDWI IEVIVEDLEV KKNFMKQINE
YRKKGSIVSS NTSGVSIDKI VEDMPLEFKQ YFLGTHFFNP PRYMKLFELI PGKDTLPEIV
QFMADFATER LGKGIVLAKD TPNFVANRIG TYALENVIQL MDKHGYDIPK LDQLTGPIIG
RPKSATFRTI DMVGLDIFYH VVGNVINNID DEEEKKQLII PKFVEELIEK GYLGDKTKQG
FYKKVKTENG AHTLVWDVKK EDYVPVIKDK VEAIEKAMQE DNKLKALVFG ETEENKFAWE
LIKNVLLYSA RKVPEIADDY REIDNAMMWG FNWEFGPFAI WDTIGFQESI KRMKQEGDNI
PKWIQERLDN GKFKFYDSNK FDIPYILISS PKNLVVKENK GAALVDIGEE VACLQFKTKG
NTITDDVIEM INLAVEEVEE SYKGLVIGNQ SKNFSSGANL ADIEKWISNK EWKSIENMCD
AFQKANMALK YCSKPVVAAP YGMTLGGGAE ITMHTYKIAA HAETYMGLVE LGVGLIPAGG
GTKELLTRSI QEAGNASIGE ILSSLRKAWN TIVMKKVSGS AHDAMKKGYI KNSDLIVMNK
DYLIDEAKKM VIYLADIGFR PLQKKGITVL GVTGRAALES DAYSLLKGEF ITEYDAHIAN
KVAYVLTGGN VSKGVILTEE QILELEKEAF LSLCGEEKTK QRIQYMLCNG KQLRN
//
