ID A0A0L9TQ51_PHAAN Unreviewed; 646 AA.
AC A0A0L9TQ51;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
GN ORFNames=LR48_Vigan01g216200 {ECO:0000313|EMBL:KOM32606.1};
OS Phaseolus angularis (Azuki bean) (Vigna angularis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX NCBI_TaxID=3914 {ECO:0000313|EMBL:KOM32606.1, ECO:0000313|Proteomes:UP000053144};
RN [1] {ECO:0000313|Proteomes:UP000053144}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Jingnong 6 {ECO:0000313|Proteomes:UP000053144};
RX PubMed=26460024; DOI=10.1073/pnas.1420949112;
RA Yang K., Tian Z., Chen C., Luo L., Zhao B., Wang Z., Yu L., Li Y., Sun Y.,
RA Li W., Chen Y., Li Y., Zhang Y., Ai D., Zhao J., Shang C., Ma Y., Wu B.,
RA Wang M., Gao L., Sun D., Zhang P., Guo F., Wang W., Li Y., Wang J.,
RA Varshney R.K., Wang J., Ling H.Q., Wan P.;
RT "Genome sequencing of adzuki bean (Vigna angularis) provides insight into
RT high starch and low fat accumulation and domestication.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:13213-13218(2015).
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC {ECO:0000256|ARBA:ARBA00004914}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-
CC pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}. Vacuole
CC lumen {ECO:0000256|ARBA:ARBA00004410}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
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DR EMBL; CM003371; KOM32606.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0L9TQ51; -.
DR STRING; 3914.A0A0L9TQ51; -.
DR EnsemblPlants; KOM32606; KOM32606; LR48_Vigan01g216200.
DR Gramene; KOM32606; KOM32606; LR48_Vigan01g216200.
DR OMA; TTFFCQD; -.
DR Proteomes; UP000053144; Chromosome 1.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005775; C:vacuolar lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18624; GH32_Fruct1-like; 1.
DR InterPro; IPR021792; Beta-fructofuranosidase_N.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR31953:SF1; ACID BETA-FRUCTOFURANOSIDASE 4, VACUOLAR; 1.
DR PANTHER; PTHR31953; BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR Pfam; PF11837; INV_N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000053144};
KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Vacuole {ECO:0000256|ARBA:ARBA00022554};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT TRANSMEM 24..43
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 4..112
FT /note="Beta-fructofuranosidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11837"
FT DOMAIN 120..435
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 438..629
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
FT REGION 50..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 646 AA; 71863 MW; A5AC69523D5A1D90 CRC64;
MEHHKPLLPT SSHAAPTPTT RKDLLFIVCG LLFLSSLVAY GGYRASGVPH THVSSPTSNH
QQDHQSPTSL LSSQWYPVSR GVSSGVSEKS SNLLFASEGG ASEAFPWDNS MLSWQRTSFH
FQPEKNWMNG PMYYKGWYHF FYQYNPNGAV WGDIVWGHAV SRDMIHWLHL PLAMVADQWY
DKQGVWTGSA TILPNGEIIM LYTGSTNESV QVQNLAYPAD PSDPLLLDWI KHTGNPVLVP
PPGIGAKDFR DPTTAWLTSE GKWRITIGSK LNKTGIALVY DTEDFKTFEL KEGLLRAVPG
TGMWECVDFF PVSKKNGNGL DTSVNGAEVK HVMKVSLDDD RHDYYAIGTY DDNKVLFTPD
DVKNDVGVGL RYDYGIFYAS KTFFDQNKNR RILWGWIGES DSEYADVTKG WASVQSIPRT
VRLDTKTGSN LLQWPVAEVE SLRLRSDEFK SLKAKPGSVV SLDIETATQL DVVAEFEIDT
ESLQKTAESN EEFTCSSSGG AAQRGALGPF GLLVLADEGL SEYTPVYFYV IKGRNGNLRT
SFCSDQSRSS QANDVRKQIF GSVVPVLKGE KFSIRMLVDH SIVESFAQGG RTCVTSRVYP
TKAIYGAARL FLFNNATEAT VTASLKVWQM NSAFIRPFPF KPDQKS
//
