ID A0A0L9UJV5_PHAAN Unreviewed; 578 AA.
AC A0A0L9UJV5;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=EF-hand domain-containing protein {ECO:0000259|PROSITE:PS50222};
GN ORFNames=LR48_Vigan05g063700 {ECO:0000313|EMBL:KOM43033.1};
OS Phaseolus angularis (Azuki bean) (Vigna angularis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX NCBI_TaxID=3914 {ECO:0000313|EMBL:KOM43033.1, ECO:0000313|Proteomes:UP000053144};
RN [1] {ECO:0000313|Proteomes:UP000053144}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Jingnong 6 {ECO:0000313|Proteomes:UP000053144};
RX PubMed=26460024; DOI=10.1073/pnas.1420949112;
RA Yang K., Tian Z., Chen C., Luo L., Zhao B., Wang Z., Yu L., Li Y., Sun Y.,
RA Li W., Chen Y., Li Y., Zhang Y., Ai D., Zhao J., Shang C., Ma Y., Wu B.,
RA Wang M., Gao L., Sun D., Zhang P., Guo F., Wang W., Li Y., Wang J.,
RA Varshney R.K., Wang J., Ling H.Q., Wan P.;
RT "Genome sequencing of adzuki bean (Vigna angularis) provides insight into
RT high starch and low fat accumulation and domestication.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:13213-13218(2015).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Vacuole membrane
CC {ECO:0000256|ARBA:ARBA00004128}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004128}.
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DR EMBL; CM003375; KOM43033.1; -; Genomic_DNA.
DR RefSeq; XP_017425637.1; XM_017570148.1.
DR AlphaFoldDB; A0A0L9UJV5; -.
DR EnsemblPlants; KOM43033; KOM43033; LR48_Vigan05g063700.
DR GeneID; 108334357; -.
DR Gramene; KOM43033; KOM43033; LR48_Vigan05g063700.
DR KEGG; var:108334357; -.
DR OMA; HTMKFLS; -.
DR OrthoDB; 8324at2759; -.
DR Proteomes; UP000053144; Chromosome 5.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0015368; F:calcium:monoatomic cation antiporter activity; IEA:UniProt.
DR CDD; cd00051; EFh; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR004837; NaCa_Exmemb.
DR PANTHER; PTHR31503:SF36; SODIUM_CALCIUM EXCHANGER NCL; 1.
DR PANTHER; PTHR31503; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 4: Predicted;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000053144};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Vacuole {ECO:0000256|ARBA:ARBA00022554}.
FT SIGNAL 1..28
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 29..578
FT /note="EF-hand domain-containing protein"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5005595777"
FT TRANSMEM 66..89
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 101..122
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 142..169
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 203..220
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 232..254
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 425..444
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 456..482
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 494..517
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 529..549
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 556..575
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 294..329
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 334..369
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
SQ SEQUENCE 578 AA; 63269 MW; 8766945477C4438E CRC64;
MTMAASLSSH RSIFLILWIV ILCGHAHARF FSRDPLSDGV SANTWEGLLR LPSAITAESS
CEQTYGFLPC TTTVIGNLFL IIVYGFLMFK AATFLSGGSE LLLEILGPGI VGGLFLPILG
ALPDAMLILV SGLSGSKETA QSQVSVGMGL LAGSTVLLLT IIWGTCVIVG KCDIENSIAI
DSRDTRGFNL TGSGVSTDIW TSYAARIMVI SVLPFLIVQL PQILNSTSGR HLAVLIALIV
SLCLLAAYCL YQIFQPWIQK RKLEYIKHKH VILGFLRHLK KRALGRLLKD NGEPDKEVIE
KLFRTIDEDQ DGSLTHSELK ALVVGIQFEE IDLDHDDAVK RIMNDFDTSG NELVDEVEFV
NGVSRWLQRA QRARVASGDA GPHTMKFLSD FHTETKREHD LLDVGDQSNE EAEGIENAKW
TSIKAVLLLL LGTIIAAAFA DPLVDVVDNF SEATSIPAFF ISFIALPLAT NSSEAVSAII
FASRDKRQTA SLTFSELYGA VTMNNVLCLS VFLALVYARG LTWDFSSEVL VILVVCLVVG
VFASFRTVFP LWTSILAILL YPFSLALVYV LDYVFGWS
//
